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Abstract

The experiment have been done on the winter farming season 2020 in one of the farms that belongs to Faris company in Basrah governorate, the technique ISSR have been used to study the genetic distance for twenty one isolates of snake melon Cucumis melo var. flexuosus species. The variations between amplified samples have been revealed after running them on a gel of agarose which have been previously stained by ethidium bromide. Five primers which gave varied product on the agarose have been selected. Those five primers produced 713 bands, both primers UBC 813 and UBC 815 showed the higher numbers of bands reached to 177 while the primer UBC862 showed the least numbers of bands (100) and the bands which showed multi-variations showed (46) bands, and the results primers amplification unique bands their number reached to (14) and five of those bands belong to the primer UBC842 while the primer UBC862 produced three bands while the primer UBC807 did not produced any bands. While it has shown 100% polymorphisms with the primers 813, 815, 842 and 862, and the least polymorphism percentage have shown with the primer UBC807 reached 75%. According to the efficiency primers, the highest efficiency percentage shown with the primer UBC813 and 815 reached to 24.82% and the least percentage shown 14.02% by the primer UBC862. Cluster analysis showed the effect on the variance of the studied cultivars.

Keywords

Cucumis melo Var flexuosus ISSR Genetic Distance

Article Details

How to Cite
Al Khazraji, H. A. K. ., Abd , A. M. ., & Abdulla, A. A. . (2021). The Determination of the Genetic Distance of Various Snake Melon Cucumis melo var. flexuosus Cultivars Using Inter Simple Sequence Repeat Technique (ISSR). Basrah J. Agric. Sci., 34(1), 111–123. https://doi.org/10.37077/25200860.2021.34.1.10

References

  1. Akash, M., Awad, N., & Kasrawi, M. (2020). Genetic diversity among snake melon landraces (Cucumis melo var. flexuosus) using molecular descriptors. Plant Biosystems-An International Journal Dealing with all Aspects of Plant Biology, 154, 206-212. https://doi.org/10.1080/11263504.2019.1587536
  2. Bashandy, T., & El-Shaieny, A. (2016). Screening of Cowpea (Vigna unguiculata L. Walp) genotypes for salinity tolerance using field evaluation and molecular analysis. Journal of Agricultural Chemistry and Biotechnology, 7, 249-255. https://jacb.journals.ekb.eg/article_41126_400cb64b104d1aea2b5411f20b2ed3c9.pdf
  3. Besirli, G., & Yanmaz, R. (1997). Types of the snake cucumber (Cucumis melo var. flexuosus Naud.) Grown in the south east region of Turkey. In International Symposium on Cucurbits 492, 37-40. ‏ https://doi.org/10.17660/ActaHortic.1999.492.3
  4. Cerasela, P., Lazar, A., Irina, P., Maria, I. B. O., Giancarla, V., & Banu, C. (2011). Somaclonal variation at the nucleotide sequence level revealed by RAPD and ISSR markers. Journal of Horticulture, Forestry and Biotechnology, 15, 119-123.‏https://www.usab-tm.ro/Journal-HFB/romana/2011/2011%203%204/Lista%20lucrari_2011%20PDF/JHFB_15(4)_PDF/29Petolescu%20Cerasela.pdf
  5. Chowdhury, M. A., Vandenberg, B., & Warkentin, T. (2002). Cultivar identification and genetic relationship among selected breeding lines and cultivars in chickpea (Cicer arietinum L.). Euphytica, 127, 317-325. https://doi.org/10.1023/A:1020366819075
  6. Grundmann, H., Schneider, C., Hartung, D., Daschner, F. D., & Pitt, T. L. (1995). Discriminatory power of three DNA-based typing techniques for Pseudomonas aeruginosa. Journal of Clinical Microbiology, 33, 528-534.‏
  7. https://jcm.asm.org/content/jcm/33/3/528.full.pdf
  8. Heiba, S. A. A., Haiba, A. A. A., & Abdel-Rahman, H. M. (2019). Determination of genetic markers in some Egyptian varieties of wheat and barley under salt and drought stresses. Asian Journal of Crop Science, 11 59-70.‏ https://doi.org/10.3923/ajcs.2019.59.70.
  9. Mahgoub, H. A., Sofy, A. R., Abdel-Azeem, E. A., & Abo-Zahra, M. S. (2016). Molecular markers associated with salt-tolerance of different soybean (Glycine max L.) cultivars under salt stress. International Journal of Advanced Research in Biological Sciences, 3, 241-267. https://ijarbs.com/pdfcopy/aug2016/ijarbs37.pdf
  10. Majeed, D. M., Ismail, E. N., Al-Mishhadani, I. I., & Sakran, N. M. (2018). Assessment of genetic diversity among wheat selected egenotypese and local varieties for salt tolerance by using eRAPD and ISSR analysis. Iraqi Journal of Science, 59, 278-286. https://doi.org/10.24996/ijs.2018.59.1B.5
  11. Mallah, O. B. Y. (2014). Assessment of biodiversity among Palestinian landraces of Cucumis melo L. groups based on morphological descriptors and molecular markers (RAPD and ISSR). Ph. D. Thesis. University of An-Najah National.https://repository.najah.edu/bitstream/handle/20.500.11888/8435/Omar%20Mallah.pdf?sequence=
  12. Munns, R., (2002). Comparative physiology of salt and water stress, plant. Cell & Environment, 25: 239-250.https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.0016-8025.2001.00808.x
  13. Nei, M., & Li, W. H. (1979). Mathematical model for studying genetic variation in terms of restriction endonucleases. Proceedings of the National Academy of Sciences. 76, 5269-5273.https://www.pnas.org/content/pnas/76/10/5269.full.pdf
  14. Paridaa, A. K., & Dass, A.B. (2005). Salt tolerance and salinity effects on plants: A review. Ecotoxicology and Environmental safety, 60, 324-349.https://doi.org/10.1016/j.ecoenv.2004.06.010
  15. Sambrook, J., & Russell, D. W. (2001). Molecular cloning: A laboratory manual. 3rd edition. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., 999pp. https://catalogue.nla.gov.au/Record/2284148
  16. Semagn K., Bjørnstad Å., Ndjiondjop, M. N. (2006) An overview of molecular marker methods for plants. African Journal of Biotechnologym 5, 2540–2568. file:///C:/Users/hp/Downloads/56080-Article%20Text-94793-1-10-20100701.pdf.
  17. Sestili, S., Daniele, A., Rosa, A., Ferrari, V., Belisario, A., Ficcadenti, N., & Pitrat, M. (2008). Molecular characterization of different Italian inodorus melon populations based on ISSR molecular markers and preliminary SSR analysis. Proceedings of IX Eucarpia Meeting on Genetics and Breeding of Cucur-bitaceae; May 21–24, Avignon, France: INRA. 307–311. https://www.semanticscholar.org/paper/Molecular-characterization-of-different-Italian-on-Sestili-Daniele/45a0e8683c6ce20c0d68e9e935a8ce3a8ea952e8?p2df
  18. Sneath, P. H., & Sokal, R. R. (1973). Numerical taxonomy. The principles and practice of numerical classification.‏ San Francisco: Freeman, 573p. http://garfield.library.upenn.edu/classics1987/A1987F272200002.pdf