Article Sidebar

The 3rd International Conference of Agricultural Sciences 27-28 March, 2019
Download
PDF
Statistic
Read Counter : 336 Download : 267

Downloads

Download data is not yet available.

Main Article Content

Abstract

Date palm (Phoenix dactylifera L.) is one of the most important fruit trees spread in southern Iraq, but because of many circumstances decreased their numbers significantly, the propagation of date palm by tissue culture was the best solution for re-preparation of the problem as it was previously, because propagation is accelerated. Summarizes the working paper in an attempt to find the optimal concentration of thidiazuran (TDZ) (0.0 , 0.5 and 1.0) mgl-1, in production of callus and regeneration of shoots, at different times of initial and callus culturing in medium budding. The results showed that the concentration of (0.5) mgl-1 was optimal in all traits under study.

Keywords

Phoenix dactylifera Thidiazuron (TDZ) Callus Shoot multiplication browning in vitro

Article Details

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

How to Cite
Al-Asadi, A. Z. ., Abdulwahid, A. H. ., & Al-Mayahi, A. M. . (2019). The Effect of Thidiazuron on Callus and in vitro Shoots Development of Date Palm (Phoenix dactylifera L.) cv. Barhee. Basrah Journal of Agricultural Sciences, 32, 258–265. https://doi.org/10.37077/25200860.2019.170
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX

References

  1. Abbasi, H.; Zeb, A.; Xu L.L. & Wei, Y.H. (2011). Thidiazuron: A multidimensional plant growth regulator. Afr. J. Biotechnol., 10(45): 8984- 9000.
  2. Ahloowalia, B.S.; Prakash, J.; Savangilkar, V.A. & Savangilkar, C. (2004). Plant tissue culture. Low cost of option for tissue culture technology in developing countries .proceeding of a technical Meeting , Organized by the Joint FAO . IAEA. 3-10.
  3. Al-Mayahi, A.M.W. (2014). Thidiazuron induced in vitro bud organogenesis of the date palm (Phoenix dactylifera L.) cv. Hillawi. Afr. J. Biotechnol., 13(35): 3581-3590.
  4. Al-Mayahi. A.M.W. (2016). Effect of red and blue light emitting diodes ‘‘CRB-LED’’ on in vitro organogenesis of date palm (Phoenix dactylifera L.) cv. Alshakr. World J. Microbiol. Biotechnol., 32: 160 https://doi.org/10.1007/s11274-016-2120-6.
  5. Al-Wasel, A.S. (2001). Phenotypic comparison of tissue culture derived and conventionally propagated by offshoots date palm (Phoenix dactylifera L.) cv. Barhee trees 1-Vegetative characteristics. J. K.S.U. Agric. Sci., 13(1): 65-73.
  6. Cappelletti, P.; Sabbadini, S. & Mezzetti, B. (2016). The use of TDZ for the efficient in vitro regeneration and organogenesis of strawberry and blueberry cultivars. Sci. Hor., 207: 117-124.
  7. Casanova, E.; Valdes, A.E.; Fernandez, B.; Moysset, L., & Trillas, M.I. (2004). Levels and immune localization of endogenous cytokinins in thidiazuron -induced shoot organogenesis in carnation. J. Plan Physiol., 161(1): 95-104.
  8. Chitra, D.S.V. & Padmaja G (2005). Shoot regeneration via direct organogenesis from in vitro derived leaves of mulberry using thidiazuron and 6-benzylaminopurine. Sci. Hortic., 106(4): 593-602.
  9. Gubbuk H. & Pekmezci, M. (2006). In vitro propagation of banana (Musa spp.) using thidiazuron and activated charcoal. Acta Agr. Scand. B S. P., 56(1): 65-69.
  10. Gueye, B.; Morcillo F.; Collin M.; Gargani D. & Overvoorde, P. (2009). Acquisition of callogeniccapacity in date palm leaf tissues in response to 2,4-D treatment. Plant Cell Tiss. Organ Cult., 99: 35-45.
  11. Jasim, A.M. & Saad, A.A. (2001). Effect of some media component on growth and somatic embryos formation and germination of date palm (Phoenix dactylifera L.) cultured in vitro. Basrah Date Palm Res. J., 1: 1-7.
  12. Jasim, A.M.; Al-Mayahi A.M.W. &. Attaha A.H.M. (2009). Propagation of four rare cultivars of date palm (Phoenix dactylifera L.) by tissue culture techniques. Basrah J. Date Palm Res., 8(1): 72-99.
  13. Jericó1 B.B.; Lourdes, I.A.; Lázaro, S.V.; José, C.M. & Nancy, S.B. (2012). In vitro regeneration of Pinus brutia Ten. var. Edarica (Medw.) through organogenesis. Afr. J. Biot., 11(93): 15982-15987.
  14. Murashig, T. & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures Physiol. Plant, 15: 473- 497.
  15. San-Jose, M.C.; Ballester, A. & Vieitez, A.M. (2001). Effect of thidiazuron on multiple shoot induction and plant regeneration from cotyledonary nodes of chestnut. J. Hort. Sci. .Biotechnol., 76(5): 588-595.
  16. Snedecor, G.M. & W.G. Cochran, (1989). Statistical Methods. 1st ed., Iowa State University, Ames:503pp.
  17. Tang, W. & Newton R.J. (2005). Plant regeneration from callus cultures derived from mature zygotic embryos in white pine (Pinus strobus). Plant Cell Rep., 24: 1-9.
  18. Thomas, T.D. (2003). Thidiazuron induced multiple shoot induction and plant regeneration from cotyledonay explants of mulberry. Biol. Plant. 46: 529–533.
  19. Wrigley, G. (1995). Date Palm Pp: 399-403. In: Smart, J. and Simonds, N.W. (Eds.). Evolution of Crop Plants 2nd ed. Longman, London: 496pp.