TY - JOUR AU - Abu-Mejdad , Najwa M.J.A. AU - Al-Badran, Adnan I. AU - Al-Saadoon, Abdullah H. PY - 2020/06/24 Y2 - 2024/03/28 TI - A Novel Report on Killer Yeast Strains Identification Methods JF - Basrah Journal of Agricultural Sciences JA - Basrah J. Agric. Sci. VL - 33 IS - 1 SE - ORIGINAL ARTICLE(S) DO - 10.37077/25200860.2020.32.1.04 UR - https://bjas.bajas.edu.iq/index.php/bjas/article/view/174 SP - 39-49 AB - <p style="text-align: justify;">This study was conducted to isolate and identify killer yeasts from soil samples that collected from different locations in Basrah and Dhi-Qar provinces. Seventy-five soil samples were collected from different areas, including sandy, arable, surface sediment and uncultivated soil, using dilution methods to cultivate a serial dilution of each soil sample. The results showed that a 112 isolates were identified biochemically using VITEK system and molecularly using internal transcribed spacer (ITS1- 5.8S-ITS2) marker. The molecular identification provided fast and precise identification results for the 112 isolates, whereas the VITEK test resulted low identification efficiency (8.2% were accurate and 91.8 % were not). The Diazonium blue B salts produced a good colour reaction in distinguishing between ascomycetes and basidiomycetes. The PCR was more accurate in identification of killer yeasts compared to the VITEK system.</p> ER -