Impact of Supplementation of Hemp Seeds (Cannavis sativa L.) on In Vitro Gas Fermentation Parameters and Digestibility using Foregut Fluid from Dromdary Camel

Authors

  • Tahereh Mohammadabadi Department of Animal Science, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran

DOI:

https://doi.org/10.37077/25200860.2020.33.1.08

Abstract

This experiment was conducted to investigate the effect of hemp seeds on in vitro biogas production, and digestibility of one-humped camel. Two female fistulated dromedary camels (300 kg) were fed with a forage diet (60 wheat straw and 40 alfalfa) for 1 month. Then rumen fluid was supplied from camels prior to the morning meal. Experimental samples were control diet and diet containing 10 % hemp seeds. Glass vials 100 ml containing 30 ml buffered rumen fluid was used. Gas production parameters were measured by exponential models and digestibility of foregut bacteria of camels were determined. The results showed that addition hemp seeds decreased potential of gas production (P<0.05), but gas production rate was not influenced (P>0.05). Actually digested organic matter and microbial biomass of diet containing hemp seeds were more than a control diet (P<0.05).While, hemp seeds supplementation had the greatest digestibility by cellulolytic bacteria of camel, after 24, 48 and 72 h incubation. The results also showed hemp seeds decreased ammonia nitrogen concentration as compared to the control treatment (P<0.05). Therefore, adding hemp seeds in the camel diet caused to reduce gas emission and ammonia nitrogen production in foregut and increased cellulolytic bacteria digestibility; so hemp seeds may be used in camel diets and will influence camel growth performance and decrease gas emissions in the environment.

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References

Alexander, G.; Singh, B.; Sahoo, A. & Bhat, T.K. (2008). In vitro screening of plant extracts to enhance the efficiency of utilization of energy and nitrogenin ruminant diets. Anim. Feed Sci. Techol., 145: 229-242. https://doi.org/10.1016/j.anifeedsci.2007.05.036

Blummel, M.; Makkar, H.P.S. & Becker, K. (1997). In vitro gas production - a technique revisited. J. Anim. Physiol. Anim. Nutr., 77: 24-34. https://doi.org/10.1111/j.1439-0396.1997.tb00734.x

Barani, M.; Afzali, N. & Hosseini-Vashan, S.J. (2017). Effects of dietary inclusion of extruded hempseed (Cannabis sativa L.) on performance, carcass components, humoral immune response and plasma lipid profile of broiler chickens. Anim. Prod. Res., 6(2): 39-49. https://doi.org/10.22124/ar.2017.2540

Bodas, R.; Fernández, M.; García-González, R.; González, J.S.; López, S. & Wallace, R.J. (2009). Phytogenic additives to decrease in vitro ruminal methanogenesis. Options Méditerranéennes. Série A, Séminaires Méditerranéens N., 85: 279- 283. http://om.ciheam.org/om/pdf/a85/00801018.pdf

Broderick, G.A. & Kang, J.H. (1980). Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media. J. Dairy Sci., 63: 64-75. https://doi.org/10.3168/jds.S0022 0302(80)82888-8

Broudisco, L.S. & Pochnet, S. (1994). Effect of linseed oil supplementation on feed degradation and microbial protein synthesis in the rumen of ciliate free and faunated sheep. Anim. Feed Sci. Technol., 49(3): 189-202.https://doi.org/10.1016/0377-8401(94)90045-0

Busquet, M.; Calsamiglia, S.; Ferret, A.; Carro, M.D. & Kamel, C. (2005). Effect of garlic oil and four of its compounds on rumen microbial fermentation. J. Dairy Sci., 88: 4393-4404. https://doi.org/10.3168/jds.S0022-0302(05)73126-X

Callaway, J.C. (2004). Hemp seed as a nutritional resource: An overview. Euphytica, 140(1): 65-72.https://doi.org/10.1007/s10681-004-4811-6

Cowieson A.J.; Acamovic T. & Bedford M.R. (2004). The effects of phytase and phytic acid on the loss of endogenous amino acids and minerals from broiler chickens. B. Poult. Sci., 45(1): 101-108.https://doi.org/10.1080/00071660410001668923

Gibb, D.J.; Shah, M.A.; Mir, P.S. & McAllister, T.A. (2005). Effect of full-fat hemp seed on performance and tissue fatty acids of feedlot cattle. Can. J. Anim. Sci., 85(2): 223-230. https://www.nrcresearchpress.com/doi/pdf/10.4141/A04-078

Hessle, A.; Eriksson, M.; Nadeau, E.; Turner, T. & Johansson, B. (2008). Cold-pressed hempseed cake as a protein feed for growing cattle. Acta Agric. Scandin. Anim. Sci., 58(3): 136-145. https://doi.org/10.1080/09064700802452192

Karmshahi, K.; Jlilvand, G.; Diani, O. & Dehghan, M. (2018). Effects of hemp seeds in diets with different levels of crude protein on performance, digestibility, ruminal metabolites and microbial protein in bluchi fattening lambs. J. Rum. Res., 7(2): 33-58.

22069/EJRR.2019.16225.1675

Mahmoudi, M.; Farhomaand, P. & Azarfar, A. (2012). Effects of different levels of cannabis (Cannabis sativa L.) on performance, internal organs weight and serum cholesterol levels in broiler chicks. Quarterly J. Medicinal Plants, 11: 121-129. (In Persian).

URL: http://jmp.ir/article-1-442-fa.html

Menk, K.H. & Steingass, H. (1988). Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Anim. Res. Develop., 28: 7-55. https://www.scienceopen.com/document?vid=e1859372-e696-424a-85fb-d305b0b594bc

Mohammadabadi, T. & Chaji, M. (2011). Effect of exogenous enzyme on in vitro fermentation of sesame straw by rumen bacteria culture. J. Appl. Anim. Res., 39: 161-163. https://doi.org/10.1080/09712119.2011.565227

Mohammadabadi, T. & Chaji, M. (2019). In vitro study of dietary supplementation of Malva sylvestris to Suaeda fruticosa plant on rumen digestibility, fermentation and protozoa morphology in one humped camel. Rum. Sci., 8(1): 1-8. http://www.anandpub.com/tag/in-vitro-study-of-dietary-supplementation-of-malva-sylvestris-to-suaeda-fruticosa-plant-on-rumen-digestibility/

Mustafa, A.F.; McKinnon, J.J. & Christensen, D.A. (1999). The nutritive value of hemp meal for ruminants. Can. J. Anim. Sci., 79: 91-95. https://www.nrcresearchpress.com/doi/pdf/10.4141/A98-031

Odani, S. & Odani, S. (1998). Isolation and primary structure of a methionine- and cystine-rich seed protein of Cannabis sativa. Biosci. Biotech. Biochemis., 62(4):650-654. https://doi.org/10.1271/bbb.62.650

Ørskov, E.R. & McDonald, I. (1979). The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage. J. Agric. Sci. (Cambridge)., 92: 499-503. https://doi.org/10.1017/S0021859600063048

Pashaei, S.; Ghorchi, T. & Yamchi, A. (2014). Effect of edible sources containing unsaturated fatty acids on diets containing different levels of energy and crude protein on growth performance and blood parameters of lambs. J. Res. Rum., 2(4): 103-120. (In Persian).

http://ejrr.gau.ac.ir/article_2140_f994a8df8fcfc4219c5217ab41d7d9f3.pdf

Paya, H.; Taghizadeh, A.; Janmohammadi, H.; Moghadam, G.A. & Hoseinkhani, A. (2015). Protozoa population and microbial protein production in sheep fed with microwave safflower seed. Res. J. Rum., 3(3): 27-39. (In Persian). https://animalscience.tabrizu.ac.ir/article_5102_f41280568a431ba600ef5d7dc8b73245.pdf

SAS. (2003). SAS User’s Guide Statistics. Version 9.1.3 ed.. SAS Inst., Inc., Cary NC.

Sallam, S.M.A.; da Silva Bueno, I.C.; de Godoy, P.B.; Eduardo, F.N.; Schmidt Vittib, D.M.S. & Abdalla, A.L. (2010). Ruminal fermentation and tannins bioactivity of some browses using a semi-automated gas production technique. Tropic. Subtropic. Agro Ecosys., 12: 1-10. http://www.revista.ccba.uady.mx/ojs/index.php/TSA/article/view/299

Silversides, F.G. & Lefrancois, M.R. (2005). The effect of feeding hemp seed meal to laying hens. B. Poult. Sci., 46(2): 231-235. https://doi.org/10.1080/0071660500066183

Sliwinski, B.J.; Soliva, C.R.; Machmuller, A. & Kreuzer, M. (2002). Efficacy of plant extracts rich in secondary constituents to modify rumen fermentation. Anim. Feed Sci. Technol., 101: 101-114. https://doi.org/10.1016/S0377-8401(02)00139-6

Tabaraki, R.; Yosefi, Z. & Asadi Gharneh, H.A. (2012). Chemical composition and antioxidant properties of Malva sylvestris L. J. Res. Agric. Sci., 8(1): 59-68. https://www.sid.ir/en/Journal/ViewPaper.aspx?ID=265942

Zandi Esfahan, E.; Assareh, M.H.; Jafari, M.; Ashraf Jafari, A.; Akbar Javadi, S. & Karimi, G. (2010). Phenological effects on forage quality of two halophyte species Atriplex leucoclada and Suaeda vermiculata in four saline rangelands of Iran. J. Food, Agric. Environ., 8(3-4): 999-1003. https://www.academia.edu/37346018/Phenological_effects_on_forage_quality_of_two_halophyte_species_Atriplex_leucoclada_and_Suaeda_vermiculata_in_four_saline_rangelands_of_Iran

Published

2020-06-27

How to Cite

Mohammadabadi, T. (2020). Impact of Supplementation of Hemp Seeds (Cannavis sativa L.) on In Vitro Gas Fermentation Parameters and Digestibility using Foregut Fluid from Dromdary Camel. Basrah J. Agric. Sci., 33(1), 98-106. https://doi.org/10.37077/25200860.2020.33.1.08

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