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Abstract

The aim of this study was to investigate the effects of Humic Acid (HA) and Ascorbic Acid (AsA) on cucumber growth under different levels of soil salinity. The experiment was designed as a factorial, using a completely randomized design in the laboratory of Samangan University in Afghanistan. The levels of salinity stress were set at (0, 50, and 100mM sodium chloride) while AsA and HA were set at (0, 30, and 60mM and 0, 1 and 2. L-l) respectively. The results of the analysis showed that the effect of all factors were significant on all studied traits. The highest mean daily germination (19%), root length (56.22mm), shoot length (39,06mm), fresh shoot weight (183.7mg), and seed vigor index were obtained from the (0mM salinity+60mM AsA+ 2g.L-l HA) treatment. Also the most germination percentage (95%), fresh root (45.88mg) and dry root (13.76mg), observed in (50mM salinity+0mM AsA+ 2g.L-l HA) treatment. However, the combined amounts of (60mM AsA+ 2g.L-l HA) were more effective for reducing different levels of salinity and increasing the growth characteristics of cucumber. Also, 2g L-l HA and 60mM AsA alone, were more effective. Treatments without AsA and HA showed the lowest growth in most traits. These findings suggest that the application of HA and AsA can help to improve cucumber growth under salt stress conditions.

Keywords

Abiotic stress Dry shoot Fresh shoot Germination and Root length

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Yaquby, A. M. ., Rabbani, B. ., & Saddad, S. . (2024). Effect of Humic Acid and Ascorbic Acid on Seed Germination and Growth of Cucumber (Cucumis sativus L.) under Salinity Stress. Basrah Journal of Agricultural Sciences, 37(1), 105–118. https://doi.org/10.37077/25200860.2024.37.1.09
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References

  1. Abdel Nabi, H. A., & Obaid, A. K. (2019). Effect of humic acid on some growth characteristics and green yield of two hybrids of broad bean (Vicia faba L.). Basrah Journal of Agricultural Sciences, 32,256–261. https://doi.org/10.37077/25200860.2019.273
  2. Abdul-Baki, A. A., & Anderson, J. D. (1973). Vigor determination in soybean seed by multiple criteria 1. Crop Science, 13(6), 630-633. https://doi.org/10.2135/CROPSCI1973.0011183X001300060013X
  3. Afzal, I., Basra, S.M.A., Ahmad, N. and Farooq, M. (2005). Optimization of hormonal priming techniques for alleviation of salinity stress in wheat (Triticum aestivum L.). Caderno de Pesquisa série Biologia,17,95-109. https://hdl.handle.net/1807/5388
  4. Ajithkumar, P. V., Gangadhara, K. P., Manilal, P., & Kunhi, A. A. M. (1998). Soil inoculation with Pseudomonas aeruginosa 3mT eliminates the inhibitory effect of 3-chloro-and 4-chlorobenzoate on tomato seed germination. Soil Biology and Biochemistry, 30(8-9).
  5. https://doi.org/10.1016/S0038-0717(97)00249-6
  6. Akinci, S., Bueyuekkeskin, T., Eroglu, A., & Erdogan, B. E. (2009). The effect of humic acid on nutrient composition in broad bean (Vicia faba L.) roots. Notulae Scientia Biologicae, 1(1), 81-87.
  7. https://doi.org/10.15835/nsb113489
  8. Akladious, S. A., & Mohamed, H. I. (2018). Ameliorative effects of calcium nitrate and humic acid on the growth, yield component and biochemical attribute of pepper (Capsicum annuum) plants grown under salt stress. Scientia Horticulturae, 236, 244-250.
  9. https://doi.org/10.1016/j.scienta.2018.03.047
  10. Allela, W. B. M., & Al-Hamdani, S. Y. H. (2019). Effect of some agricultural treatments on chemical and qualitative characters of five cucumber hybrids grown under unheated greenhouse. Basrah Journal of Agricultural Sciences, 32, 47–58.
  11. https://doi.org/10.37077/25200860.2019.139
  12. Al-madhagi, I. (2019). Effect of humic acid and yeast on the yield of greenhouse cucumber. Journal of Horticulture and Postharvest Research, 2(1), 67-82.
  13. https://doi.org/10.22077/jhpr.2018.1773.1029
  14. Alsaeedi, A., El-Ramady, H., Alshaal, T., El-Garawani, M., Elhawat, N., & Al-Otaibi, A. (2018). Exogenous nanosilica improves germination and growth of cucumber by maintaining K+/Na+ ratio under elevated Na+ stress. Plant Physiology and Biochemistry,125,164-171.
  15. https://doi.org/10.1016/j.plaphy.2018.02.006
  16. Anjum, N. A., Gill, S. S., Gill, R., Hasanuzzaman, M., Duarte, A. C., Pereira, E., & Tuteja, N. (2014). Metal/metalloid stress tolerance in plants: role of ascorbate, its redox couple, and associated enzymes. Protoplasma, 251, 1265-1283.
  17. https://doi.org/10.1007/s00709-014-0636-x
  18. Athar, H. R, Khan, A., & Ashraf, M. (2008). Exogenously applied ascorbic acid alleviates salt-induced oxidative stress in wheat. Environmental and experimental botany, 63(1-3), 224-231.
  19. https://doi.org/10.1016/j.envexpbot.2007.10.018
  20. Atiyeh, R. M., Lee, S., Edwards, C. A., Arancon, N. Q., & Metzger, J. D. (2002). The influence of humic acids derived from earthworm-processed organic wastes on plant growth. Bioresource Technology,84(1),7-14.
  21. https://doi.org/10.1016/S0960-8524(02)00017-2
  22. Awad, A. A. E-. A. M., & Ahmed, H. M. H. (2020). Influence of humic substances on cucumber seeds storability and root rot diseases incidence under salinity conditions. International Journal of Plant & Soil Science, 32(1), 51-73.
  23. https://doi.org/10.9734/IJPSS/2020/v32i130235
  24. Barth, C., De Tullio, M., & Conklin, P. L. (2006). The role of ascorbic acid in the control of flowering time and the onset of senescence. Journal of experimental botany, 57, 1657-1665.
  25. https://doi.org/10.1093/jxb/erj198
  26. Behairy, R. T., El-Danasoury, M., & Craker, L. (2012). Impact of ascorbic acid on seed germination, seedling growth, and enzyme activity of salt-stressed fenugreek. Journal of Medicinally Active Plants, 1, 106-113. https://doi.org/10.7275/R5TT4NW9
  27. Canellas, L. P., Olivares, F. L., Okorokova-Façanha, A. L., & Façanha, A. R. (2002). Humic acids isolated from earthworm compost enhance root elongation, lateral root emergence, and plasma membrane H+-ATPase activity in maize roots. Plant Physiology, 130(4), 1951-1957.
  28. https://doi.org/10.1104/pp.007088
  29. Chen, Y., & Schnitzer, M. (1978). The surface tension of aqueous solutions of soil humic substances. Soil Science Society of America, 125, 7-15.
  30. https://doi.10.1097/00010694-197801000-00002
  31. Demir, K., Günes, A., Inal, A., & Alpaslan, M. (1997). Effects of humic acids on the yield and mineral nutrition of cucumber (Cucumis sativus L.) grown with different salinity levels. In I International Symposium on Cucurbits 492, 95-104.
  32. https://doi.org/10.17660/ActaHortic.1999.492.11
  33. Hartwigsen, J. A., & Evans, M. R. (2000). Humic acid seed and substrate treatments promote seedling root development. HortScience, 35(7), 1231-1233.
  34. https://doi.org/10.21273/HORTSCI.35.7.1231
  35. Ibrahim, E. A. (2016). Seed priming to alleviate salinity stress in germinating seeds. Journal of Plant Physiology, 192, 38-46.
  36. https://doi.org/10.1016/j.jplph.2015.12.011
  37. Jaraghili, P. M., Shoja, H. M., & Kazemi, E. M. (2016). Evaluation of the effect of salinity on the germination and expression of antioxidant genes in two cultivars of tomato plant. Genetic Engineering and Biosafety Journal, 5, 51-59.
  38. http://gebsj.ir/article-1-122-en.html
  39. Kadhim, J. J., & Hamza, J. H. (2021). Effect of seeds soaking and vegetative parts nutrition with acids of ascorbic, citric and humic on maize growth. Iraqi Journal of Agricultural Sciences, 52(5), 1207-1218.
  40. https://doi.org/10.36103/ijas.v52i5.1458
  41. Larcher, W. (2003). Physiological plant ecology: ecophysiology and stress physiology of functional groups. Springer Science and Business Media, 514pp.
  42. https://doi.org/10.1023/B:BIOP.0000041119.93332.43
  43. Maach, M., Akodad, M., Moumen, A., Skalli, A., Hmeid, H. A., Gueddari, H., & Baghour, M. (2021). Bio-regulators: silicon, salicylic acid, ascorbic acid improve salt tolerance in cucumber (Cucumis sativus L.). Science Publishing Group, 1(1), 1-7.
  44. https://doi.org/10.11648/j.ajbio.20210906.16
  45. Mora, V., Bacaicoa, E., Zamarreno, A. M., Aguirre, E., Garnica, M., Fuentes, M., & Garcia-Mina, J.M. (2010). Action of humic acid on promotion of cucumber shoot growth involves nitrate-related changes associated with the root-to-shoot distribution of cytokinins, polyamines and mineral nutrients. Journal of Plant Physiology, 167, 633–642. https://doi.org/10.1016/j.jplph.2009.11.018
  46. Noreen, S., Sultan, M., Akhter, M. S., Shah, K. H., Ummara, U., Manzoor, H., Ulfat, M., Alyemeni, M. N., & Ahmad, P. (2021). Foliar fertigation of ascorbic acid and zinc improves growth, antioxidant enzyme activity and harvest index in barley (Hordeum vulgare L.) grown under salt stress. Plant Physiology and Biochemistry, 158, 244-254.
  47. https://doi.org/10.1016/j.plaphy.2020.11.007
  48. Pourmeidani, A., Naeini, M., Bagheri, H., & Karimi, G. (2011). Investigation on salinity tolerance of three rangeland grasses in greenhouse condition. Iranian journal of Range and Desert Reseach. 18, 58-70.
  49. https://doi.org/10.22092/ijrdr.2011.102000
  50. Ranal, M. A., & Santana, D. G. D. (2006). How and why to measure the germination process?. Brazilian Journal of Botany, 29, 1-11.
  51. https://doi.org/10.1590/S0100-84042006000100002
  52. Rasouli, M., & Noroozisharaf, A. (2022). Effects of humic acid and salinity stress on some germination and morpho-physiological indices of Iranian St John’s Wort in in vitro conditions. Journal of Crops Improvement, 24(4), 1293-1310.
  53. https://doi.org/10.22059/jci.2022.328340.2593
  54. Rohani, N. S., Nimati, S. H., & Moqadam, M. (2016). The effect of humic acid on seed germination and seedling growth characteristics of three varieties of radish in salinity stress. Iranian Seed Science and Research, 3, 29-41.
  55. https://dorl.net/dor/20.1001.1.24763780.1395.3.4.3.3
  56. Sato, S., Sakaguchi, S., Furukawa, H., & Ikeda, H. (2006). Effects of NaCl application to hydroponic nutrient solution on fruit characteristics of tomato (Lycopersicon esculentum Mill.). Scientia Horticulturae, 109, 248-253.
  57. https://doi.org/10.1016/j.scienta.2006.05.003
  58. Scott, S. J., Jones, R. A., & Williams, W. (1984). Review of data analysis methods for seed germination 1. Crop science, 24(6), 1192-1199.
  59. https://doi.org/10.2135/cropsci1984.0011183X002400060043x
  60. Sen, S. K., & Mandal, P. (2016). Solid matrix priming with chitosan enhances seed germination and seedling invigoration in mung bean under salinity stress. Journal of Central European Agriculture, 17, 749-762. https://doi.org/10.5513/JCEA01/17.3.1773
  61. Karakurt, Y., Ozdamar-Unlu, H., Unlu, H., & Tonguc, M. (2015). Antioxidant compounds and activity in cucumber fruit in response to foliar and soil humic acid application. European Journal of Horticultural Science, 80(2), 76-80.
  62. http://doi.org/10.17660/eJHS.2015/80.2.5
  63. Sofi, A., Ebrahimi, M., & Shirmohammadi, E. (2018). Effect of humic acid on germination, growth, and photosynthetic pigments of Medicago sativa L. under salt stress. Ecopersia, 6(1), 21-30.
  64. http://dorl.net/dor/20.1001.1.23222700.2018.6.1.3.8
  65. Türkmen, Ö., Dursun, A., Turan, M., & Erdinç, Ç. (2004). Calcium and humic acid affect seed germination, growth, and nutrient content of tomato (Lycopersicon esculentum L.) seedlings under saline soil conditions. Acta Agriculturae Scandinavica, Section B-Soil & Plant Science, 54, 168-174. https://doi.org/10.1080/09064710310022014
  66. Volkov, V., & Beilby, M. J. (2017). Salinity tolerance in plants: Mechanisms and regulation of ion transport. Frontiers in Plant Science, 8, 1795.
  67. https://doi.org/10.3389/fpls.2017.01795
  68. Weerasekara, I., Sinniah, U. R., Namasivayam, P., Nazli, M. H., Abdurahman, S. A., & Ghazali, M. N. (2021). Priming with humic acid to reverse ageing damage in soybean [Glycine max (L.) Merrill.] seeds. Agriculture, 11(10),966.
  69. https://doi.org/10.3390/agriculture11100966
  70. Zu, X., Lu, Y., Wang, Q., Chu, P., Miao, W., Wang, H., & La, H. (2017). A new method for evaluating the drought tolerance of upland rice cultivars. The Crop Journal, 5(6), 488–498.
  71. https://doi.org/10.1016/j.cj.2017.05.002