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Abstract

This study was conducted to identify Fusarium spp. isolated from tomato plant in Iraq and China. A total of 12 isolates from Iraq (1-12) and four isolates from China (M1-M4) were used in this study. Based on Morphological characteristics (color, growth pattern, Macro and Micro-conidia shape) high differences between Fusarium isolates were found. Sensitivity test to the fungicide carbendazim revealed that one third of isolates(4/12) from Iraq had EC50 values over than 1000?g/ml indicated that many Iraqi isolates have developed resistance to carbendazim. Based on ITS sequences, Fusarium isolates were identified as follow, isolates 1, 3, 5, 6, 7, 10 were identified as Fusarium oxysporum, isolates 8, 11 as F. solani, isolates 12, M1 and M3 as F. moniliforme, isolates 2, M2 and M4 as F. proliferatum, F. chlamydosporum and F. kyushuense , respectively.

Keywords

RAPD-PCR Fusarium spp tomato plant Iraq China

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How to Cite
Fayyadh, M. A., Al-Badran, A. I., & Al-Jaafari, I. S. (2017). Molecular identification of Fusarium spp. isolated from tomato plant in Iraq and China. Basrah Journal of Agricultural Sciences, 30(1), 65–72. https://doi.org/10.37077/25200860.2017.23
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References

  1. Al-halo, Y.I. (1995). Some fungi associated with Tomato roots and their relation with host growth and damping-off caused by Fusarium oxysporum f. sp. lycopersici .M. Sc Thesis. Coll. Agriculture. Univ. Basrah: 62pp.
  2. AttitAlla, I.H. (2004). Biological and molecular characteristics of microorganism-stimulated defense response in Lycopersicon esculentum. Ph.D. Dissertation, Fac. Science and Technology, Acta Univ. Upsaliensis, Uppsala, Sweden: 82pp.
  3. Balogun, O.S. (2007). Comparison of Fusarium oxysporum f. sp. lycopersici races 1, 2, 3 and f. sp. radicis lycopersici based on the sequences of fragments of the ribosomal DNA intergenic spacer region. Biochemistry, 19(1): 1-8.
  4. Booth, C. (1971). The genus Fusarium. Common wealth institute. Kew Surrey. England. 237pp.
  5. Cai, G.; Gale, L.R.; Schnider, R.W.; Kistler, H.C.; Davis, R.M.; Elias, K.S. and Miyao, E.M. (2003). Origin of races 3 of Fusarium oxysporum f. sp. lycopersici at a single site in California. Phytopathology, 93: 1014- 1022.
  6. Di Pietro, A.; Madrid, M.P.; Caracuel, Z.; Delgado-Jarana, J. and Roncero, M.I.G. (2003). Pathogen profile Fusarium oxysporum: Exploring the molecular arsenal of vascular wilt fungus. Mol. Plant Pathol., 4: 315-325.
  7. El-Fadly, G.B and El-Kazzaz, M.K. (2008). Identification of some Fusarium spp. Using RAPD-PCR Technique. Egypt J. Pathology, 36: 71-80.
  8. El-Kazzaz, M.K.; El-Fadly, G.B.; Hassan, M.A.A and El-Kot, G.A.N. (2008). identification of some Fusarium spp. using molecular biology techniques .Egypt. J. Phytopathology, 36: 71-80.
  9. Hirano, Y. and Arie, T. (2006). PCR-based differentiation of Fusarium oxysporum f. sp. lycopersici and radicis lycopersici and races of F. oxysporum f. sp. lycopersici. J. Gen. Plant Patho., 72: 273-283.
  10. Iqbal, Z.; Pervez, M.A.; Ahmad, S.; Iftkar, Y.; Yasin, M.; Nawas, A.; Ghazanf, M.U.; Dasti, A.A and Saleem, A. (2010). Determination of minimum inhibitory concentration of fungicides against fungus Fusarium mangifera. Pak. J. Bot., 42(5): 3525-3532.
  11. Karkachi, N.; Gharbi, S.; Kihal, M. and Henni, J.E. (2010). Biological control of Fusarium oxysporum f. sp. lycopersici isolated from Algerian tomato by Psedomonas fluorescens, Bacillus cereus, Serratia marcenscens and Trichoderma harzianum. Research Journal of Agronomy, 4(2): 31-34.
  12. Leong, S.K.; Latiffah, Z. and Baharuddin, S. (2010). Molecular characterization of Fusarium oxysporum f. sp. cubense of Banana. American Journal of Applied Science, 6: 1301-1307.
  13. Messian, C.; Blancard, D.; Rouxel, F.; and Lafon, P. (1991). Diseases of the Market- Gardening plants. I.N.R.A. Paris. 387pp.
  14. Matlob, A.N.; Mohammed, I.S. and Abdol, K.S. (1989). Production of vegetables Vol. 2. Min. Higher Education and Scientofic Research. Univ. Ninevah. 165pp.
  15. Ramantthan ,G.; Banupriya , S. and Abirami , D. (2010). Production and optimization of cellulase from Fusarium oxysporum by submerged fermentation. Journal of Science and industrial Research, 69: 454- 459.
  16. Tort, N.; Ozturk, L. and Tosun, N. (2004). The effect of Fungicide application on anatomical structure and Physiology of Tomato (Lycopersicon esculentum Mill). J. Agric., 41(2): 111-122.
  17. Vakalounakis, D.J and Fragkiadakis, G.A. (1998). Genetic diversity of Fusarium oxysporum isolates from Cucumber differentiation by pathogenicity, vegetative compatibility and RAPD fingerprinting. Phytopathology, 89: 161-168.