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Incubation studies were conducted to reveal effected heavy metals (Cd, Cr, Cu, Fe, Mn, Ni, Pb and Zn) add at critical concentrations to soils with different texture on urease activity and thermodynamic parameters (Ea and Q10) incubated under different temperatures (10, 20, 30, 40, 50, 60 and 70) °C for 14 days under field capacity and waterlogged moisture levels. Urease activity was measured and thermodynamic parameters were calculated. Results showed that the urease activity increased with increasing temperature of incubation from 10 to 50°C then the activity decreased as temperature increased above 50°C at both moisture levels and at all heavy metals treatments. Increasing moisture level from field capacity to water-logged significantly (P=0.05) decreased urease activity, while increased Ea value, at all heavy metals treatments. The soil texture significantly affected urease activity and thermodynamic parameters (Ea and Q10). Results also indicated that effect of heavy metals on urease and thermodynamic parameters differed according to the soil temperature and the moisture level.


Urease Ea Q10 Heavy metals Moisture levels soil

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Tfaij, A. L. ., Al-Ansari , A. M. S., & Al- Jaberi, M. M. . (2019). Activity and Thermodynamic Parameters of Urease Enzymes in Soils Treated with Some Heavy Metals Under Different Temperatures and Moisture Levels. Basrah Journal of Agricultural Sciences, 32, 193–206.


  1. Al-Tawil, L.S.J. (2016). The effect of fertilizers in Amidohydrolases enzymes in sunflower (Helianthus annuus) Rhizoshper. Qadisiyah J. Sci. Biol., 6)2(: 165-173. (In Arabic).
  2. Al-Ansari, A.S. (2000). Characteristics of enzymes in recently reclaimed land Urease activity and kinetics parameters. Basrah J. Sci. Biol., 18: 111-122.
  3. Al-Ansari, A.S.; Abdulkareem, M.A. & Kadhum, S.J. (2019). Activity and thermodynamic parameters of urease in soil amended with organic residues. Iraqi J. Agric. Sci., 50(3):
  4. Al-Jaberi, M.M. (2010). Amidohydrolases activity, kinetic and thermodynamic parameters in some marsh and southern Iraqi soils. Ph. D. Thesis. Coll. Agric., Univ. Basrah: 221pp. (In Arabic).
  5. Al-Harakani, H.T.H. (2018). Soil bio-reclamation treated with some heavy metals using locally isolated fungi and its effect on biological and enzymatic activity. M. Sc. Thesis, Coll. Agriculture, Univ. Basrah: 151pp. (In Arabic).
  6. Busto, M.D. & Perez-Mateos M. (2000). Characterization of B-D-glacosidase extracted from soil fractions. Eur. J. Soil Sci., 51: 193-200.
  7. Burke, D.J.; Weintraub, M.N.; Hewins, C.R. & Kalisz, S. (2011). Relationship between soil enzyme activities, nutrient cycling and soil fungal communities in a northern hardwood forest. Soil Biol. Biochem., 43: 795-803.
  8. Ciarkowska K, & Gambus´ F. (2004). Activity of dehydrogenase in heavy metal contaminated soils in the area of Olkusz Zesz. Probl. Post. Nauk. Rol., 501: 79-87.
  9. Ciarkowska, K.; So?ek-Podwika, K. & Wieczorek, J. (2014). Enzyme activity as an indicator of soil rehabilitation processes at a zinc and lead ore mining and processing area. J Environ Manage. 132: 250-256.
  10. Cordero, I.; Snell, H. & Richard, D.B. (2019). High throughput method for measuring urease activity in soil. Soil Biol. Biochem., 134: 72–77.
  11. Dick, R.P. (1997). Soil Enzyme Activities as Integrative Indicators of Soil Health. In: Pankhurst, C. E., Doube, B. M., Gupta, V.V.S.R. (eds.) Biological Indicators of Soil Health, CABI Publishing. USA.
  12. Dick, W.A. & Tabatabai, M.A. (1999). Use of Immobilized Enzymes for Bioremediation. 315-338. In: ASSA (ed.). Bioremediation of Contaminated Soils. Agronomy Monograph 37, Madison: 820pp.
  13. Follmer, C. (2008). Insights into the role and structure of plant ureases. Phytochemistry 69(1): 18-28.
  14. Frankenberger, W.T. & Tabatabai, M.A. (1980). Amidase activity in soils: II. Kinetic parameters. Soil. Sci. Soc. Am. J., 44: 532-536.
  15. Fraser, F.C.; Hallett, P.D.; Wookey, P.A.; Hartley, I.P. & Hopkins, D.W. (2013). How do enzymes catalyzing soil nitrogen transformations respond to changing temperatures. Biol. Fertil. Soils, 49: 99-103.
  16. Gu, C.; Zhang, S.; Han, P.; Hu, X.; Xie, L.; Li, Y.; Brooks, M.; Liao, X. & Qin, L. (2019). Soil enzyme activity in soils subjected to flooding and the effect on nitrogen and phosphorus uptake by oilseed rape. Front. Plant Sci., 10: 368. doi: 10.3389/fpls.2019.00368.
  17. Hang X.; Li, F.; Liu, T.; Xu, C.; Duan, D.; Peng, C.; Zhu, S. & Shi, J. (2013). The variations in the soil enzyme activity, protein expression, microbial biomass and community structure of soil contaminated by heavy metals. Int. Scholarly Res. Notices, 2013: 803150.
  18. Henry, H.A.L. (2012). Soil extracellular enzyme dynamics in a changing climate. Soil Biol. Biochem., 47: 53-59.
  19. Kabata-Pendias, A. & Pendias, H. (2001). Trace Elements in Soil and Plant. 3rd C.R.C. Press: 413pp.
  20. Kizilkaya, R. & Ekberli, I. (2008). Determination of the effects of hazelnut husk and tea waste treatments on urease enzyme activity and its kinetics in soil. Turk. J. Agric., 32: 299-310.
  21. Krajewska, B. (2009). Urease1, functional, catalytic and kinetic properties: Areview. J. Mol. Catal. B. Enzym., 59: 9-21.
  22. Kumari, J.A. & Rao, P.C. (2017). Effect of temperature on soil enzymes urese activity-productivity. College of Agriculture, Rajendra Nagar, Hyderabad, In., 5(4): 65-72.
  23. Maroney, M.J. & Ciurli, S. (2013). Nonredox nickel enzymes. Chem. Rev., 114(8): 4206-4228.?
  24. Machuca, A.; Cuba-Díaz, M. & Córdova, C. (2015). Enzymes in the rhizosphere of plants growing in the vicinity of the polish Arctowski antarctic station. J. Soil Sci. Plant Nutr., 15: 833-838.
  25. Meng, F.X.; Ou, W.; Li Q.; Jiang, Y. & Wen, D.Z. (2006). Vertical distribution and seasonal fluctuation of nematode tropic groups as affected by land use. Pedosphere, 15: 204-215.
  26. Meng, X.; Yingwei A.; Ruirui L. & Wenjuan Z. (2018). Effects of heavy metal pollution on enzyme activities in railway cut slope soils. 197 Page 2 of 12. Environ. Monit. Assess. 190: 197.
  27. Ofoegbu, C.J.; Akubugwo, E.I.; Dike, C.C. Maduka, H.C.C.; Ugwu, C.E. & Obasi, N.A. (2013). Effects of Heavy Metals on Soil Enzymatic Activities in the Ishiagu Mining Area of Ebonyi State-Nigeria. IOSR J. Environ. Sci. Toxicol. Food Technol., 5(6): 66-71.
  28. Oliveira, A., & Pampulha, M.E. (2006). Effects of long-term heavy metal contamination on soil microbial characteristics. J. Biosci. Bioeng., 102(3): 157-161.
  29. Ou, Y.; Alain, N.; Rousseau, L.W.; Baixing, Y.; Thiago, G. & Hui, Z. (2019). Identification of the alteration of riparian wetland on soil properties, enzyme activities and microbial communities following extreme flooding. Geoderma, 337: 825-833.
  30. Pulford, I.D. & Tabatabai M.A. (1988). Effect of waterlogging on enzyme activities in soils. Soil. Boil. Biochem., 20: 215-219.
  31. Page, A.L.; Miller, R.H. & Keeney, D.R. (1982). Methods of soil analysis. Part 2. 2nd. Ed. ASA. Inc. Madison, Wisconsin: 1158pp.
  32. Setter, T.L.; Waters, I.; Sharma, S.K.; Singh, K.N.; Kulshreshtha, N. & Yaduvanshi, N. P.S. (2009). Review of wheat improvement for waterlogging tolerance in Australia and India: the importance of anaerobiosis and element toxicities associated with different soils. Ann. Bot., 103: 221-235.
  33. Tabatabai, M.A. (1994). Soil Enzymes. 775-833. In: Bottomley, P.S.; Angle, J.R. & Weaver, R.W. (Eds.). Methods of Soil Analysis. Part 2: Microbiological and Biochemical Properties. Soil Sci. Soc. Am., Madison, WI: 1121pp.
  34. Tabatabi, M.A. & Bremner, J.M. (1972). Assay of urease activity in soils. Soil. Biol. Biochem., 4: 479-487.
  35. Wyszkowska, J.; Zaborowska, A. & Kucharski, J. (2006). Activity of enzymes in zinc contaminated soil. EJPAU Environ. Develop., 9(1): 1-9.
  36. Yang, Z.; Liu, S.; Zheng, D. & Feng, S. (2006). Effects of cadmium, zinc and lead on soil enzyme activities. J. Environ. Sci. 18(6): 1135-1141.
  37. Zaher, A.T.; Hadhili, K.H. & Fakhir, S.J. )2010(. Effect of some heavy metals on the effectiveness of microorganisms and urease enzyme activity in the soil. Basrah J. Agric. Sci.,, 23(2): 214-225. (In Arabic).
  38. Zafren, E. & Hall, P.L. (1973). The Study of Enzyme Mechanisms. John Wiley and Sons. Inc. New York: 284pp.
  39. Zhang, F.P.; Li, C.F.; Tong, L.G.; Yue, L.X. & Li, P. (2010). Response of microbial characteristics to heavy metal pollution of mining soils in central Tibet, China. Appl. Soil Ecol., 45: 144-151.
  40. Zhang, N.; Pan, R.R.; Zhou, Z.H.; Liu, Y.; Jiang, J. & Tian, Q. (2016). Effects of full wettish cultivation and waterlogging cultivation on soil nutrient content and enzyme activities. Acta Agric. Jiangxi, 28: 28-31.