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Abstract

This study was conducted to isolate and identify killer yeasts from soil samples that collected from different locations in Basrah and Dhi-Qar provinces. Seventy-five soil samples were collected from different areas, including sandy, arable, surface sediment and uncultivated soil, using dilution methods to cultivate a serial dilution of each soil sample. The results showed that a 112 isolates were identified biochemically using VITEK system and molecularly using internal transcribed spacer (ITS1- 5.8S-ITS2) marker. The molecular identification provided fast and precise identification results for the 112 isolates, whereas the VITEK test resulted low identification efficiency (8.2% were accurate and 91.8 % were not). The Diazonium blue B salts produced a good colour reaction in distinguishing between ascomycetes and basidiomycetes. The PCR was more accurate in identification of killer yeasts compared to the VITEK system.

Keywords

Diazonium blue B VITEK PCR Killer yeasts Diagnosis

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Abu-Mejdad , N. M. ., Al-Badran, A. I. ., & Al-Saadoon, A. H. (2020). A Novel Report on Killer Yeast Strains Identification Methods. Basrah Journal of Agricultural Sciences, 33(1), 39–49. https://doi.org/10.37077/25200860.2020.32.1.04
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