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Abstract
The agricultural production processes currently targeted reducing chemical fungicides usage and increasing bio-agent application through controlling diseases alone or integrating it with other factors. The study aimed to investigate the induction of systemic resistance by multi bio-agents represented by mycorrhizal fungi Glomus mosseae, G. intradicas and Trichoderma harizanum against pathogenic fungus Rhizoctonia solani which caused wilt disease and growth defoliation to Okra seedling. Three isolate of R. solani were recorded on root of Okra seedling, named (local - Batra). Isolate no. (3) was more virulence than other isolates in damping off disease in the pre and post emergence. Results also showed that G. mosseae and G. intradicas with T. harizanum had a positive influence in reducing detrimental effect of R. solani in all growth parameters (e.g. fresh and dry weight of root) on disease severity on Okra plant caused by R. solani. Bio-agents (G. mosseae,G. intradicas and T. harizanum) increased resistance in Okra plants by raising production of enzymescatalase and Peroxidase.this experiment was revealed that using a complex of bio-agent’s factors were greatly increase the efficiency of biological control than using each of them individually. We conclude that the broad diversity of rhizosphere micro-organisms as well as the confronting between the bio-chemical and physical changes could be reflected the variations in the metabolic secondary products that could inhibit pathogens.
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References
- Aebi, H. (1984). Catalase in vitro. Method Enzyme, 105: 121-126.
- Al-Obaidy, D. I. (2007). Improve efficiency of T. viride by using oligosaccharide to control accompanying fungi with Okra seed. M. Sc. Thesis. Coll. Education, Univ. Mosul: 210pp.
- Al-Rawei, K.M. & Khalaf Allah, A.M. (1980). Design and analysis of agriculture experiments. Ministry of Higher Education and Scientific Research. House of book Press. Univ. Baghdad: 488pp.
- Al-Taie, A.H.; Matrood, A.A. & Al-Asadyi, M. (2016). The influence of some Fungi bio-genic on promoting growth and yield of wheat-var. Ibaa 99. Int. J. Curr. Microbiol. App. Sci., 5(11): 757-764.
- Alwan, S.L. (2005). Possibility of manufacturing bio-pesticide from Trichoderma harzianum Rifai to control root rot and damping-off wheat. Ph.D. Thesis, Coll. Education. Univ. Kufa: 260pp.
- Aly, A.A.; Abdel-Sattar, M.A. & Omar, M.R. (2007). Differential antagonism of Trichoderma sp. against Macrophomina phaseolina. J. Plant Prot. Res., 47(2): 91- 102.
- Barnett, H.C. & Hunter B.B. (2006). Illustrated genera of imperfect Fungi. Burgess Publishing Company. Minnesota: 241pp.
- Bell, D. K.; Wells, H. D. & Markham, C.R. (1982). In vitro antagonism of Trichoderma species against six fungal plant pathogens. Phytopathology, 72(4): 379-382.
- Dawar, S.; Hayat, S.; Anis, M. & Zaki, M.J. (2008). Effect of seed coating material in the efficacy of microbial antagonists for the control of root rot fungi on okra and sunflower. Pak. J. Bot., 40(3): 1269-1278.
- Dewan, M.M. & El-Behadili, A.H. (1985) Plant Pathology. Technical Institution, Baghdad. 344pp.
- Duffy, B.K.; Simon, A. & Weller, D.M. (1996). Combination of Trichoderma koningli with Pseudomonas fluorescent for control of take all on wheat. Phytopathology, 86: 188-194.
- El-Fiki A.I.I.; Mohamed F.G.; El-Deeb A.A. & Khalifa, M.M.A. (2004). Some applicable methods for controlling sesame charcoal rot disease (Macrophomina phaseolina) under greenhouse conditions, Egypt. J. Phytopathol., 32(1-2): 87-101.
- Katatny, M.H.; Somitsch, W.; Robra, K.H; El-Katatny, M.S. & Gubitz, G.M. (2000). Production of chitinase and B-1,3- glucanase by Tridoderma harzianum for control of the phytopathogenic fungus Sclerotium rolfsii. Food Technol. Biotechnol., 38: 173-180.
- Eman, F. S.; Abd El-Aziz, Q. & El-Deeb, M. (2012). Bio-recycling of shrimp shellby Trichoderma viride for production of anti- fungal chitinase. Afr. J. Microbiol. Res., 6(21): 4538-4545.
- Fakir, G.A. (2000). An annotated list of seed borne diseases in Bangladesh Seed Pathology Laboratory. Dept. Plant Pathology. Bangladesh Agriculture Univ. Mymensingh, Bangladesh: 187pp.
- Finlay, R.D. (2008). Ecological aspects of mycorrhizal symbiosis with special emphasis on the functional diversity of interactions involving the extra radical mycelium. J. Exp. Bot., 59(5): 1115-1126.
- Gailite, A.; Steinite, I. & Ievinsh, G. (2005). Ethylene is involved in Trichoderma induced resistance of bean plants against Pseudomonas syringae. Biology, 691: 59-70.
- Hafez, F.T. (1992). Planting vegetable. Ministry of higher education and scientific research. Basrah Univ.: 495pp.
- Hamid, F.R. (2002). Study efficiency of isolates of Trichoderma spp. in inducing resistance against Rhizoctonia solani in four variety of cotton. M. Sc. Thesis. Coll. Educ., Univ. Baghdad, Iraq: 233pp.
- Hammond-Kosack, K.E & Jones, J.D.G. (1996). Resistance gene-dependent plant defense responses. Plant Cell, 8: 1773-
- Harman, G.E. (2006). Overview of mechanisms and uses of Trichoderma spp. Phytopathology, 96(2): 190.
- Hibar, K.; Daami, M. & El-Mahjoud, M. (2007). Introduction of resistance in tomato plants against Fusarium oxysporum f. sp. Radices lycopersici by Trichodermas spp. Tunisian, J. Pl. Protect, 2: 47-58.
- Howell, C.R.; Hanson, L.E.; Stipanovic, R.D. & Puckhober, L.S. (2000). Introduction of terpenoid synthesis in cotton roots and control of Rhizoctonia solani seed treatment with Trichoderma virens. Phytopathology, 35: 49-60.
- Jayalakshmi, R.; Raju, S.; Usha, R & Sreeramula, K. (2009). Trichoderma harzianumL, as a potential source for lytic enzymes and elicitor of defense responses in chickpea (Cicer arietinum L.) against wilt disease caused by Fusarium oxysporum f. sp. Cicero. Aust. J. Crop Sci., 1: 44-52.
- Kim, S.H.; Terry, M.E.; Hoops, P.; Dauwalder, M. & Roux, S.J. (1988). Production and characterization of monoclonal antibodies to wall-localized peroxidases from corn seedlings. Plant Physiol., 88: 1446-1453.
- Metcalf, D.D. & Wilson, C.C. (2001). The process of antagonism of Sclerotium cepivorum in white rot affected onion roots by Trichoderma koningii. Plant Pathol., 50: 249-257.
- Rini, C.R. & Sulochana, K.K. (2007). Usefulness of Trichoderma and Pseudomonas against Rhizoconia solani and Fusarium oxysporum infecting tomato.J. Trop. Agric., 45: 21-28.
- Shafique, .H.A.; Sultana, R.N. & Ara, J. (2015). Effect of endophytic Pseudomonas aeruginosa and Trichoderma harzianum on soil-borne diseases, Mycorrhizae and induction of systemic resistance in Okra grown in soil amended with Vernoniaan the lmintica (L.) seed’s powder Pak. J. Bot., 47(6): 2421-2426.
- Sheriff, F.M. (2012). Principles of fungi- fungal Ecology. Althakerra printing office: 608pp.
- Sharon, E.; Bar-Eyai, M.; Chet, I.; Hewrra- Estrella, A.; Kleifeld, O. & Spiegal, Y. (2001). Biological control of the rootknot nematode Meloidogyne javanica by Trichoderma harzianum. Phytopathol., 91: 687-693.
- Siameto, E.N.; Okoth, S.; Amugune, N.O. & Chege, N.C. (2010). Antagonism of Trichoderma harzianum isolates on soil borne plant pathogenic fungi from Embu District, Kenya. J. Yeast Fungal Res., 1(3): 47-54.
- Singh, B.N.; Singh, A. & Singh, B.R. (2013). Trichoderma harzianum elicits induced resistance in sunflower challenged by Rhizoctonia solani. J. Appl. Microbiol., 116(3): 654-666.
- Sirin, U. (2011). Determining the effects of Trichoderma harzianum and some mycorrhizal fungi on plant growth and against Rhizoctania solani Kühn in Lilium under in vivo conditions. J. Biotechnol., 10(67): 15142-15150.
- Takur, M. and Sohal, B.S. (2013). Role of elicitors in inducing resistance in plants against pathogen infection. Hinadwi Publ. Corporation. ISRN Biochemistry. Vol. 2013, Article ID 762412: 10pp.
- Tawajin, A.M. (1979). Greenhouse environment. Univ. Basrah: 600pp.
- Vinale, F.; Marra, R.; Scala, F.; Ghisalberti, E.L.; Lorito, M. & Sivasithamparam, K. (2006). Major secondary metabolites produced by two commercial Trichoderma strains active against different phytopathogens. Lett. Appl. Microbiol., 43: 143-148.
- Viterbo, A.; Inbar, J.; Hadar, Y. and Chet, I. (2007). Plant disease biocontrol and induced resistance via fungal mycoparasites. Pp.127-146. In: Kubicek, C.P. and Druzhinina, I.S. (Eds.). Environment and microbial relationships 2nd ed. The Mycota IV. Springer- Verlag Berlin Heidelberg: 450pp.
- Wahid, O.A.A. (2006). Improving control of Fusarium wilt of leguminous plants by combined application of biocontrol agents. Phytopathol. Mediterr., 45: 231-237.
- Wheeler, B.E.J. (1970). An introduction to plant disease. John Wiley and Sons. Ltd. London: 374pp.
References
Aebi, H. (1984). Catalase in vitro. Method Enzyme, 105: 121-126.
Al-Obaidy, D. I. (2007). Improve efficiency of T. viride by using oligosaccharide to control accompanying fungi with Okra seed. M. Sc. Thesis. Coll. Education, Univ. Mosul: 210pp.
Al-Rawei, K.M. & Khalaf Allah, A.M. (1980). Design and analysis of agriculture experiments. Ministry of Higher Education and Scientific Research. House of book Press. Univ. Baghdad: 488pp.
Al-Taie, A.H.; Matrood, A.A. & Al-Asadyi, M. (2016). The influence of some Fungi bio-genic on promoting growth and yield of wheat-var. Ibaa 99. Int. J. Curr. Microbiol. App. Sci., 5(11): 757-764.
Alwan, S.L. (2005). Possibility of manufacturing bio-pesticide from Trichoderma harzianum Rifai to control root rot and damping-off wheat. Ph.D. Thesis, Coll. Education. Univ. Kufa: 260pp.
Aly, A.A.; Abdel-Sattar, M.A. & Omar, M.R. (2007). Differential antagonism of Trichoderma sp. against Macrophomina phaseolina. J. Plant Prot. Res., 47(2): 91- 102.
Barnett, H.C. & Hunter B.B. (2006). Illustrated genera of imperfect Fungi. Burgess Publishing Company. Minnesota: 241pp.
Bell, D. K.; Wells, H. D. & Markham, C.R. (1982). In vitro antagonism of Trichoderma species against six fungal plant pathogens. Phytopathology, 72(4): 379-382.
Dawar, S.; Hayat, S.; Anis, M. & Zaki, M.J. (2008). Effect of seed coating material in the efficacy of microbial antagonists for the control of root rot fungi on okra and sunflower. Pak. J. Bot., 40(3): 1269-1278.
Dewan, M.M. & El-Behadili, A.H. (1985) Plant Pathology. Technical Institution, Baghdad. 344pp.
Duffy, B.K.; Simon, A. & Weller, D.M. (1996). Combination of Trichoderma koningli with Pseudomonas fluorescent for control of take all on wheat. Phytopathology, 86: 188-194.
El-Fiki A.I.I.; Mohamed F.G.; El-Deeb A.A. & Khalifa, M.M.A. (2004). Some applicable methods for controlling sesame charcoal rot disease (Macrophomina phaseolina) under greenhouse conditions, Egypt. J. Phytopathol., 32(1-2): 87-101.
Katatny, M.H.; Somitsch, W.; Robra, K.H; El-Katatny, M.S. & Gubitz, G.M. (2000). Production of chitinase and B-1,3- glucanase by Tridoderma harzianum for control of the phytopathogenic fungus Sclerotium rolfsii. Food Technol. Biotechnol., 38: 173-180.
Eman, F. S.; Abd El-Aziz, Q. & El-Deeb, M. (2012). Bio-recycling of shrimp shellby Trichoderma viride for production of anti- fungal chitinase. Afr. J. Microbiol. Res., 6(21): 4538-4545.
Fakir, G.A. (2000). An annotated list of seed borne diseases in Bangladesh Seed Pathology Laboratory. Dept. Plant Pathology. Bangladesh Agriculture Univ. Mymensingh, Bangladesh: 187pp.
Finlay, R.D. (2008). Ecological aspects of mycorrhizal symbiosis with special emphasis on the functional diversity of interactions involving the extra radical mycelium. J. Exp. Bot., 59(5): 1115-1126.
Gailite, A.; Steinite, I. & Ievinsh, G. (2005). Ethylene is involved in Trichoderma induced resistance of bean plants against Pseudomonas syringae. Biology, 691: 59-70.
Hafez, F.T. (1992). Planting vegetable. Ministry of higher education and scientific research. Basrah Univ.: 495pp.
Hamid, F.R. (2002). Study efficiency of isolates of Trichoderma spp. in inducing resistance against Rhizoctonia solani in four variety of cotton. M. Sc. Thesis. Coll. Educ., Univ. Baghdad, Iraq: 233pp.
Hammond-Kosack, K.E & Jones, J.D.G. (1996). Resistance gene-dependent plant defense responses. Plant Cell, 8: 1773-
Harman, G.E. (2006). Overview of mechanisms and uses of Trichoderma spp. Phytopathology, 96(2): 190.
Hibar, K.; Daami, M. & El-Mahjoud, M. (2007). Introduction of resistance in tomato plants against Fusarium oxysporum f. sp. Radices lycopersici by Trichodermas spp. Tunisian, J. Pl. Protect, 2: 47-58.
Howell, C.R.; Hanson, L.E.; Stipanovic, R.D. & Puckhober, L.S. (2000). Introduction of terpenoid synthesis in cotton roots and control of Rhizoctonia solani seed treatment with Trichoderma virens. Phytopathology, 35: 49-60.
Jayalakshmi, R.; Raju, S.; Usha, R & Sreeramula, K. (2009). Trichoderma harzianumL, as a potential source for lytic enzymes and elicitor of defense responses in chickpea (Cicer arietinum L.) against wilt disease caused by Fusarium oxysporum f. sp. Cicero. Aust. J. Crop Sci., 1: 44-52.
Kim, S.H.; Terry, M.E.; Hoops, P.; Dauwalder, M. & Roux, S.J. (1988). Production and characterization of monoclonal antibodies to wall-localized peroxidases from corn seedlings. Plant Physiol., 88: 1446-1453.
Metcalf, D.D. & Wilson, C.C. (2001). The process of antagonism of Sclerotium cepivorum in white rot affected onion roots by Trichoderma koningii. Plant Pathol., 50: 249-257.
Rini, C.R. & Sulochana, K.K. (2007). Usefulness of Trichoderma and Pseudomonas against Rhizoconia solani and Fusarium oxysporum infecting tomato.J. Trop. Agric., 45: 21-28.
Shafique, .H.A.; Sultana, R.N. & Ara, J. (2015). Effect of endophytic Pseudomonas aeruginosa and Trichoderma harzianum on soil-borne diseases, Mycorrhizae and induction of systemic resistance in Okra grown in soil amended with Vernoniaan the lmintica (L.) seed’s powder Pak. J. Bot., 47(6): 2421-2426.
Sheriff, F.M. (2012). Principles of fungi- fungal Ecology. Althakerra printing office: 608pp.
Sharon, E.; Bar-Eyai, M.; Chet, I.; Hewrra- Estrella, A.; Kleifeld, O. & Spiegal, Y. (2001). Biological control of the rootknot nematode Meloidogyne javanica by Trichoderma harzianum. Phytopathol., 91: 687-693.
Siameto, E.N.; Okoth, S.; Amugune, N.O. & Chege, N.C. (2010). Antagonism of Trichoderma harzianum isolates on soil borne plant pathogenic fungi from Embu District, Kenya. J. Yeast Fungal Res., 1(3): 47-54.
Singh, B.N.; Singh, A. & Singh, B.R. (2013). Trichoderma harzianum elicits induced resistance in sunflower challenged by Rhizoctonia solani. J. Appl. Microbiol., 116(3): 654-666.
Sirin, U. (2011). Determining the effects of Trichoderma harzianum and some mycorrhizal fungi on plant growth and against Rhizoctania solani Kühn in Lilium under in vivo conditions. J. Biotechnol., 10(67): 15142-15150.
Takur, M. and Sohal, B.S. (2013). Role of elicitors in inducing resistance in plants against pathogen infection. Hinadwi Publ. Corporation. ISRN Biochemistry. Vol. 2013, Article ID 762412: 10pp.
Tawajin, A.M. (1979). Greenhouse environment. Univ. Basrah: 600pp.
Vinale, F.; Marra, R.; Scala, F.; Ghisalberti, E.L.; Lorito, M. & Sivasithamparam, K. (2006). Major secondary metabolites produced by two commercial Trichoderma strains active against different phytopathogens. Lett. Appl. Microbiol., 43: 143-148.
Viterbo, A.; Inbar, J.; Hadar, Y. and Chet, I. (2007). Plant disease biocontrol and induced resistance via fungal mycoparasites. Pp.127-146. In: Kubicek, C.P. and Druzhinina, I.S. (Eds.). Environment and microbial relationships 2nd ed. The Mycota IV. Springer- Verlag Berlin Heidelberg: 450pp.
Wahid, O.A.A. (2006). Improving control of Fusarium wilt of leguminous plants by combined application of biocontrol agents. Phytopathol. Mediterr., 45: 231-237.
Wheeler, B.E.J. (1970). An introduction to plant disease. John Wiley and Sons. Ltd. London: 374pp.