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Water heavy metals contamination is an international issue due to its detrimental impact on the environment, aquatic organisms and impairment of the ecological balance systems. Cadmium is a heavy metal that is extremely toxic, even in low doses, thus it is a big concern to the ecosystem. Cd can cause several Patho-morphological changes in various organs in fish. This study was evaluated the effect of sublethal concentrations of Cd toxicity and effects on Histopathological of muscle tissues of Planiliza abu Juvenile. Series of Cd concentrations (0.5- 2) ppm were used and the 15d LC50 was 0.4 ppm. Several histopathological alterations were observed which increase gradually with increased concentration, included, marked thickening and hypertrophy of muscle bundle were will observed  in lowest concentration (0.5 ppm). While in the highest concentration (2 ppm),  atrophy, splitting, and separation with intracellular edema were marked  in fish muscle bundle.


Planiliza abu Cadmium Sublethal concentrations Muscles Histopathology

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Hashim , E. S. ., & Al-Yassein, R. N. . (2020). Effect of Sublethal Concentrations of Cadmium on the Histo-pathological Changes of Muscles of Planiliza abu Juveniles (Heckel, 1843). Basrah Journal of Agricultural Sciences, 33(2), 207–217.


  1. Abo El Ella, S. M., Hosny, M. M., & Bakry, M. F. (2005). Utilizing fish and aquatic weeds infestation as bioindicators for water pollution in Lake Nubia, Sudan. Egyptian Journal of Aquatic Biology and Fisheries, 9: 63-84.
  2. Alak, G., Atamanalp, M., Topal, A., Arslan, H., Kocaman, E. M., & Oruç, E. (2013a). Effect of sub-lethal lead toxicity on the histopathological and antioxidant enzyme activity of rainbow trout (Oncorhynchus mykiss). Fresenius Environmental Bulletin, 22(5), 733-738.‏
  3. Alak, G., Atamanalp, M., Topal, A., Arslan, H., Oruç, E., & Altun, S. (2013b). Histopathological and biochemical effects of humic acid against cadmium toxicity in brown trout gills and muscles. Turkish Journal of Fisheries and Aquatic Sciences, 13(2), 315-320.‏
  4. Al-Balawi, H. F. A., Al-Akel, A. S., Al-Misned, F., Suliman, E. A. M., Al-Ghanim, K. A., Mahboob, S., & Ahmad, Z. (2013). Effects of sub-lethal exposure of lead acetate on histopathology of gills, liver, kidney and muscle and its accumulation in these organs of Clarias gariepinus. Brazilian Archives of Biology and Technology, 56, 293-302.‏
  5. Aktar, M. W., Sengupta, D., & Chowdhury, A. (2011). Occurrence of heavy metals in fish: a study for impact assessment in industry prone aquatic environment around Kolkata in India. Environmental Monitoring and Assessment, 181, 51-61. https://
  6. Amdur, M.O., Doull, J., Curtis, M.D. & Kloasen, C.D. (1992). Cassarett and Doull's Toxicology. Pergamon: The Basic Science of Poisons. New York/Oxford. (p. 996).
  7. Ates, B., Orun, I., Talas, Z. S., Durmaz, G., & Yilmaz, I. (2008). Effects of sodium selenite on some biochemical and hematological parameters of rainbow trout (Oncorhynchus mykiss Walbaum, 1792) exposed to Pb 2+ and Cu 2+. Fish Physiology and Biochemistry, 34(1), 53-59.
  8. Authman, M. M., Abbas, W. T., & Gaafar, A. Y. (2012). Metals concentrations in Nile tilapia Oreochromis niloticus from illegal fish farm in Al-Minufiya Province, Egypt, and their effects on some tissues structures. Ecotoxicology and Environmental Safety, 84, 163-172.‏
  9. Badr, A. M., Mahana, N. A., & Eissa, A. (2014). Assessment of heavy metal levels in water and their toxicity in some tissues of Nile Tilapia (Oreochromis niloticus) in River Nile basin at Greater Cairo, Egypt. Global Veterinaria, 13(4), 432-443.‏
  10. Bai, L. Y., Zeng, X. B., Li, L. F., Chang, P. E. N., & Li, S. H. (2010). Effects of land use on heavy metal accumulation in soils and sources analysis. Agricultural Sciences in China, 9(11), 1650-1658.
  11. Begum, A., Mustafa, A. I., Amin, M. N., Banu, N., & Chowdhury, T. R. (2013). Accumulation and histopathological effects of arsenic in tissues of shingi fish (Stinging Catfish) Heteropneustes fossilis (Bloch, 1794). Journal of the Asiatic Society of Bangladesh, Science, 39, 221-230.‏
  12. Bernet, D., Schmidt, H., Meier, W., Burkhardt‐Holm, P., & Wahli, T. (1999). Histopathology in fish: proposal for a protocol to assess aquatic pollution. Journal of Fish Diseases, 22, 25-34.
  13. Beširović, H., Alić, A., Prašović, S., & Drommer, W. (2010). Histopathological effects of chronic exposure to cadmium and zinc on kidneys and gills of brown trout (Salmo trutta morph. fario). Turkish Journal of Fisheries and Aquatic Sciences, 10, 255-262. https:// 10.4194/trjfas.2010.0214
  14. Biswas, S., Prabhu, R. K., Hussain, K. J., Selvanayagam, M., & Satpathy, K. K. (2012). Heavy metals concentration in edible fishes from coastal region of Kalpakkam, southeastern part of India. Environmental Monitoring and Assessment, 184, 5097-5104.
  15. Bradl, H. (Ed.). (2005). Heavy Metals in the Environment: Origin, Interaction and Remediation. Elsevier. Volume 6, Academic Press, 282pp.
  16. Ceyhun, S. B., Şentürk, M., Yerlikaya, E., Erdoğan, O., Küfrevioğlu, Ö. İ., & Ekinci, D. (2011). Purification and characterization of carbonic anhydrase from the teleost fish Dicentrarchus labrax (European seabass) liver and toxicological effects of metals on enzyme activity. Environmental Toxicology and Pharmacology, 32, 69-74.
  17. Das, B. K., & Mukherjee, S. C. (2000). A histopathological study of carp (Labeo rohita) exposed to hexachlorocyclohexane. Veterinarski Arhiv, 70, 169-180.
  18. Ebrahimi, M. & Taherianfard, M. (2011). The effects of heavy metals exposure on reproductive systems of cyprinid fish from Kor River. Iranian Journal of Fisheries Sciences, 10, 13-26.‏
  19. Ferreira, M., Caetano, M., Antunes, P., Costa, J., Gil, O., Bandarra, N. & Reis-Henriques, M. A. (2010). Assessment of contaminants and biomarkers of exposure in wild and farmed sea bass. Ecotoxicology and Environmental Safety, 73, 579-588.
  20. George, S. G., Todd, K. & Wright, J. (1996). Regulation of metallothionein in teleosts: induction of MTmRNA and protein by cadmium in hepatic and extrahepatic tissues of a marine flatfish, the turbot (Scophthalmus maximus). Comparative Biochemistry and Physiology Part C: Pharmacology, Toxicology and Endocrinology, 113, 109-115.
  21. Hassan, B. K. (2005). The Effect of Sub Lethal Concentration of Cadmium on Gill and Liver of Carassian carp Carassius carassius (L.). M. Sc. Thesis, College of Agriculture, University of Basrah, 53pp. (In Arabic).
  22. Hassan, B. K. (2011). The effect of copper and cadmium on oxygen consumption of the juvenile common carp, Cyprinus carpio (L.). Mesopotamian Journal of Marine Science, 26, 25-34. DOI:10.21276/mjms
  23. Humason, G. L. (1979). Animal Tissue Techniques. 4th edition, W.H. Freeman and company, San Francisco, CA, 661pp.
  24. Ibrahim, S. M., Shalloof, K. A., & Salama, H. M. (2008). Effect of environmental conditions of Abu-Zabal Lake on some biological, histological and quality aspects of fish. Global Veterinaria, 2, 257-270.
  25. Jawad, L., Bobori, D., Al-Shwikh, H., & Al-Saleh, F. (2015). Opercular girth, maximum girth and total length relationships for Planiliza abu (Heckel, 1843) and Chondrostoma regium (Heckel, 1843) (Actinoprerygii) from Euphrates River at Dier Ez-Zor Governorate, Syria. Acta Zoolgica. Bulgaria, 67, pdf
  26. Karadeniz, A., Cemek, M., & Simsek, N. (2009). The effects of Panax ginseng and Spirulina platensis on hepatotoxicity induced by cadmium in rats. Ecotoxicology and Environmental Safety, 72, 231-235.
  27. Landis, W., Sofield, R., Yu, M. H. & Landis, W. G. (2003). Introduction to Environmental Toxicology Impacts of Chemicals Upon Ecological Systems. Third edition, CRC Press, 512pp.
  28. Mantovi, P., Baldoni, G., & Toderi, G. (2005). Reuse of liquid, dewatered, and composted sewage sludge on agricultural land: effects of long-term application on soil and crop. Water Research, 39, 289-296.
  29. McHugh, K. J., Smit, N. J., Van Vuren, J. H. J., Van Dyk, J. C., Bervoets, L., Covaci, A., & Wepener, V. (2011). A histology-based fish health assessment of the tigerfish, Hydrocynus vittatus from a DDT-affected area. Physics and Chemistry of the Earth, Parts A/B/C, 36, 895-904.
  30. Mohamed, F. A. (2008). Bioaccumulation of selected metals and histopathological alterations in tissues of Oreochromis niloticus and Lates niloticus from Lake Nasser, Egypt. Global Veterinaria, 2, 205-218.‏ 10.1.1. 499 .3845&rep=rep1&type=pdf
  31. Mohamed, F. A. (2009). Histopathological studies on Tilapia zillii and Solea vulgaris from Lake Qarun, Egypt. World Journal of Fish and Marine Sciences, 1, 29-39.
  32. Patnaik, B. B., Howrelia, H., Mathews, T., & Selvanayagam, M. (2011). Histopathology of gill, liver, muscle and brain of Cyprinus carpio communis L. exposed to sublethal concentration of lead and cadmium. African Journal of Biotechnology, 10, 12218-12223. ‏
  33. Popek, W., Dietrich, G., Glogowski, J., Demska-Zakeś, K., Drag-Kozak, E., Sionkowski, J., Łuszczek-Trojan, E., Epler, P., Demianowicz, W., Sarosiek, B., Kowalski, R., Jankun, M., Zakeś, Z., Król, J., Czerniak, S., & Szczepkowski, M. (2006). Influence of heavy metals and 4-nonylphenol on reproductive function in fish. Reproductive Biology, 6, 175-188.
  34. Qiu, Y. W., Lin, D., Liu, J. Q., & Zeng, E. Y. (2011). Bioaccumulation of trace metals in farmed fish from South China and potential risk assessment. Ecotoxicology and Environmental Safety, 74, 284-293.
  35. Raissy, M., Ansari, M., & Rahimi, E. (2011). Mercury, arsenic, cadmium and lead in lobster (Panulirus homarus) from the Persian Gulf. Toxicology and Industrial Health, 27, 655-659.
  36. Roberts, R. J. (2001). Fish Pathology, Third edition. W. B. Saunders, Philadelphia, PA, 462pp.
  37. Shukla, V., Dhankhar, M., Prakash, J., & Sastry, K. V. (2007). Bioaccumulation of Zn, Cu and Cd in Channa punctatus. Journal of Environmental Biology, 28, 395.
  38. Singh, R. K., Chavan, S. L., & Sapkale, P. H. (2007). Heavy metal concentrations in water, sediments and body tissues of red worm (Tubifex spp.) collected from natural habitat Toxicology & Pharmacology, 147(2), 168-178.s in Mumbai, India. Environmental Monitoring and Assessment, 129, 471-481.
  39. Soares, S. S., Martins, H., Gutiérrez-Merino, C., & Aureliano, M. (2008). Vanadium and cadmium in vivo effects in teleost cardiac muscle: metal accumulation and oxidative stress markers. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 147, 168-178.
  40. Sobha, K., Poornima, A., Harini, P., & Veeraiah, K. (2007). A study on biochemical changes in the freshwater fish, Catla catla (Hamilton) exposed to the heavy metal toxicant cadmium chloride. Kathmandu University Journal of Science, Engineering and Technology, 3, 1-11.
  41. Taylor, M. D. (1997). Accumulation of cadmium derived from fertilizers in New Zealand soils. Science of the Total Environment, 208(1-2), 123-126.
  42. Vieira, C., Morais, S., Ramos, S., Delerue-Matos, C., & Oliveira, M. B. P. P. (2011). Mercury, cadmium, lead and arsenic levels in three pelagic fish species from the Atlantic Ocean: Intra-and inter-specific variability and human health risks for consumption. Food and Chemical Toxicology, 49, 923-932.
  43. Vinodhini, R., & Narayanan, M. (2008). Bioaccumulation of heavy metals in organs of freshwater fish Cyprinus carpio (Common carp). International Journal of Environmental Science & Technology, 5, 179-182.
  44. Vosylienė, M. Z., & Jankaitė, A. (2006). Effect of heavy metal model mixture on rainbow trout biological parameters. Ekologija, 4, 12-17.
  45. Wright, D. A., & Welbourn, P. (2002). Environmental Toxicology (Vol. 11). Cambridge University Press, 630pp.