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Abstract
The study was carried on 68 ewes of local Awassi sheep in the Al-Kafeel sheep station Karbala governorate, Iraq for the period from 1/10/2017 to 1/8/2018. Genetic analysis was carried out in the molecular genetics laboratory at the College of Agriculture / Basrah University in order to extract (DNA) and determine the genotype of the IGF-1(Insulin-like growth factor 1) and GH (Growth Hormone) genes. This study aimed to know the association between the genotype of the ewes of IGF-1 and GH genes and their relationship to the productive traits of the lambs, which included the weights of lambs from birth to weaning and body measurements, Three of the genotypes for IGF-1 were revealed AG, GC, CC and two for GH CT, TC . The effect of IGF-1 genotypes was significant (P<0.05) on weights of lambs at weaning, age 6 months and some body measurements with superior genotype GC. The genotypes of GH had a significant effect on the weight of the lambs at birth by CT superiority. While the weights of weaning, six months and body measurements were not significantly affected by different genotypes.
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References
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- Al-Barzinji, Y.M.S. & Othman, G.U. (2013). Genetic polymorphism in FecB gene in Iraqi sheep breeds using RFLP-PCR technique. IOSR J. Agric. Vet. Sci., 12(5): 46-48.
- Al-Mahdawi, M.K.K. (2011). Effect of using different levels of protein in the diet and period of fattening on body dimensions in the Iraqi lambs. Diyala J. Agric. Sci., 3(1): 38-50.
- Al-Sahuki, B.T.J.; Al-Rawiand, A.A. & Abbas, A.A. (2017). Study of growth hormone gene and its relationship to weaning weight in goat. J. Anbar Agric. Sci., 2(9): 23-30 .
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- Chunxiang, Z.; Wei, Z. & Hailing, L. (2008). A new single nucleotide polymorphism in the IGF-I gene and its association with growth traits in the Nanjiang Huang goat. Asian-Aust. J., 4(2): 51-56.
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- Othman, E.O.; Sally, S.A.; Heba, A.M.A., & Omaima, M.A. (2015). Genotyping of growth hormone gene in Egyptian. small rumi breeds. Biotechnology,14 (3): 136-141.
- Rothschild, M.F. & Soller, M. (1997). Candidate gene analysis to detect genes controlling traits of economic importance in domestic livestock. Probe, 8: 13-20.
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References
Abu-Rahef, M. (2013). The effect on the performance in sheep. J. Feed Agr. Sci., 5(8): 67-72.
Al-Barzinji, Y.M.S. & Othman, G.U. (2013). Genetic polymorphism in FecB gene in Iraqi sheep breeds using RFLP-PCR technique. IOSR J. Agric. Vet. Sci., 12(5): 46-48.
Al-Mahdawi, M.K.K. (2011). Effect of using different levels of protein in the diet and period of fattening on body dimensions in the Iraqi lambs. Diyala J. Agric. Sci., 3(1): 38-50.
Al-Sahuki, B.T.J.; Al-Rawiand, A.A. & Abbas, A.A. (2017). Study of growth hormone gene and its relationship to weaning weight in goat. J. Anbar Agric. Sci., 2(9): 23-30 .
Al-Salhi, A.A.J. (2016). Relationship between BMP (13one morphogenetic protein 15) and GH (Growth Hormone) genes polymorphism and some production and reproductive trait in Awassi sheep. M. Sc. Thesis. Univ. Baghdad: 143pp.
Cauveri, D.; Sivaselvam, S.N. & Karthickeyan, S.M.K. (2016). Single nucleotide polymorphisms in GH gene associated with growth traits in nilagiri sheep of Tamil Nadu. Int. J. Sci. Env. Technol., 5(6): 4097-4103.
Chunxiang, Z.; Wei, Z. & Hailing, L. (2008). A new single nucleotide polymorphism in the IGF-I gene and its association with growth traits in the Nanjiang Huang goat. Asian-Aust. J., 4(2): 51-56.
Clustal, O. (2018). http://www .ebi.ac.uk/ /Tools/msa/clustalo/.
Ewa, G., Bronis?aw, B.; Piotr, J.; Jan, K. & S?awomir, M. (2017). Effect of the IGF-I gene polymorphism on growth, body size, carcass and meat quality traits in Coloured Polish Merino sheep. Arch. Anim. Breed., 6(5): 161-173.
Gadelha, M.R., Kasuki, L., & Korbonits, M. (2012). Novel pathway for somatostatin analogs in patients with acromegaly. Trends Endocrinol. Metab., 24: 238-246.
Gholibeikifard, A., Aminafshar, M. & Mashhadi, M. (2013). Polymorphism of IGF-I and ADRB3 Genes and Association with Growth Traits in the Iranian Baluchi Sheep. Agr. Sci. Tech., 15: 1153-1162.
Iraqi statistical book (2011). Ministry of Agriculture. Republic of Iraq. 260pp.
Ivan, F., Gorlov, Y.A., Kolosov, N.V., & Shirokova, L.V. (2017). Association of the growth hormone gene polymorphism with growth traits in Salsk sheep breed Ivan. Small Rum. Res., 1(5): 11-14.
Jit, L.P., Zhang, J.P., Wu, Z.J., & Ha., W.W.(2014) Study of the correlation between GH gene polymorphism and growth traits in sheep. Genet. Mol. Res., 13 (3): 7190-7200.
Masoud, N., Abbas, H. & Marziyeh A. (2013). PCR-SSCP Variation of IGF1 and PIT1 Genes and Their Association with Estimated Breeding Values of Growth Traits in Makooei Sheep. Genet. Res. Int., 23(1); 65-70.
Mojtaba, T. & Mehdi, V.V. (2009). Association of the polymorphism in the 5’ flanking region of the ovine IGF-I gene with growth traits in the Baluchi sheep. S. Afr. J. Anim. Sci., 3(5): 34-40.
Moradian, C.; Nooshin, M. & Seyed, A.S. (2013). Effects of genetic polymorphismat the growth hormone gene on growth traits in Makooei sheep. Eur. J. Exp. Biol., 3(3): 101-105.
Moradian, C.; Giti, E. & Abbas, H. (2013). Polymorphismat of IGF-1 in Makooei sheep using PCR-SSCP. Eur. J. Exp. Biol., 3(2): 490-494.
Naicy, T.; Thirupathy, V.; Thazhathuveettil, A.; Kunniyoor, C.R.; Mangattumuruppel, M. & Kulangara, S. (2017). Association of a novel single nucleotide polymorphism at the exon-2 of Insulin-Like Growth Factor 1 (IGF1) gene with phenotypic variants in goats. Vet. Arhiv., 87(4): 457-472.
Othman, E.O.; Sally, S.A.; Heba, A.M.A., & Omaima, M.A. (2015). Genotyping of growth hormone gene in Egyptian. small rumi breeds. Biotechnology,14 (3): 136-141.
Rothschild, M.F. & Soller, M. (1997). Candidate gene analysis to detect genes controlling traits of economic importance in domestic livestock. Probe, 8: 13-20.
Senegal, R.H.N. (2014). Study of growth traits related genes using PCR technique and genetic damage using comet assay technique in local Turkish and crossbred Awassi sheep. Ph. D Thesis. Coll. Agric. Univ. Baghdad: 198pp.
Spss, (2013). Statistical Package of Social Science .Ver. 22. Application Guide .Copy right by Spss Inc.Sun, W.R.; Musa, H.H.; Ding, J.T. & Kong,Y. (2014). Developmental changes in IGF- I and MyoG gene expression and their association with meat traits in sheep. Genet. Mol. Res., 13(2): 2772-2783.
Trukhachev, V.V.; Andrey, S.K.; Marina, S. & Arslan, O. (2016). Polymorphisms of the IGF-1 gene in Russian sheep breed and Their influence on some meat production parameters. Slov. Vet. Res.,53(2):77-83.