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Abstract

This study was conducted in Nutrition Lab. Department of Animal Production, College of Agriculture, Al-Qasim Green University to investigate the effect of type and level of substitution of urea with ruminant manure (sheep, cow and buffalo) on basis of nitrogen content on microbial composition of rice straw silage. Urea was substituted with dried manure at six combinations, 100:0, 90:10, 80:20, 70:30, 60:40 and 50:50. Silage samples were prepared by treating chopped straw with pre-treated solution contained 10% low quality juice and 2% urea. Treated straw was packed in double plastic bags which were closed tightly and fermented for 60 days in bits. Results revealed that samples prepared by addition of cow manure were characterized with higher number of total anaerobic (P?0.05) and lactic acid bacteria (P?0.01), 9.22 and 8.62 log CFU.g-1 FM respectively. Whereas, lower (P?0.01) number of molds and yeasts were detected in those prepared with addition of buffalo manure, 3.51 and 4.54 log CFU.g-1  FM respectively. Significant increases were also observed in the total number of anaerobic bacteria and lactic acid bacteria with lower (P?0.01) numbers of total aerobic bacteria due to substitution of urea with manure, however, lower (P?0.01) number of molds and yeasts, 3.49 and 4.51 log CFU.g-1 FM were detected in samples prepared with a combination of 100:0 of urea: manure. 

Keywords

Rice straw Manure Silage Urea Microbes

Article Details

How to Cite
Saeed, A. A. ., & Abid, S. I. . (2019). Effect of Substitution of Urea with Different Types and Levels of Ruminant Manure on Microbial Evaluation of Rice Straw Silage. Basrah Journal of Agricultural Sciences, 32, 70–79. https://doi.org/10.37077/25200860.2019.141

References

  1. Abid, S.I. (2018). Effect of substitution of urea with different ratios of ruminant manure to improve nutritive value of rice straw silage. M. Sc. Thesis. Univ. Al- Qasim Green: 105pp.
  2. Addah, W.; Baaha, J.E.; Okineb, K.; Owensd, F.N. & McAllistera, T.A. (2014). Effects of chop-length and a ferulic acid esterase- producing inoculant on fermentation and aerobic stability of barley silage, and growth performance of finishing feedlot steers. Anim. Feed Sci. Tech., 197: 34-46.
  3. Adesoji, A.T.; Ogunjobi, A.A.; Fagade, O.E. & Babayemi, O.J. (2010). Effect of Lactobacillus plantarum starter culture on the microbial succession, chemical composition, aerobic stability and accept- ability by ruminant of fermented Panicum maximum grass. Asian-Aust. J.T., 14(1): 11-24.
  4. Akintokun, A.; Jolaosho A.O. & Afolabi, R.O. (2014). Effect of L. Plantarum and carbohydrase on microbes and composition of T. Procumbens silage. Arch. Zootec., 56: 145-156.
  5. AOAC, (2004). Official Methods for Analysis of Association officials of analysis chemists. 11th ed. Washington. D.C.: 375pp.
  6. Borquez J.L.; Gonzalez-Munoz, S.S.; Pinos- Rodriguez, J.M.; Dom?nguez, I.; Barcena, J.R.; Mendoza, G.D.; Cobos, M.A. Bueno, G. (2009). Feeding value of ensiling fresh cattle manure with molasses or bakery byproducts in lambs. Livestock Sci., 122(2-3): 276-280.
  7. Borquez, J.L.; Pinos-Rodríguez, J.M.; González, S.S.; Domínguez, I.; Bárcena, R. Mendoza, G. & Cobos, M. (2010). Use of different kind of silage dairy cattle manure in lamb nutrition. Italian J. Anim. Sci., 9(25): 129-133.
  8. Catchpoole, V.R. (1970). The silage fermentation of some tropical pasture plants. Proc. 11th Int. Grass. Congr., Queensland, Australia, Univ. Queensland Press, St. Lucia: 956pp.
  9. Hall, D.G. & Keys, M.J. (1980). Evaluation of poultry manure as a nitrogen supplem- ent to diets of oats and roughages for sheep. Aust. J. Exp. Agric. Anim. Husb., 20(105): 427-432.
  10. Harrigan, W.F. & McCane, M.E. (1976). Laboratory Methods in Food and Microbiology. 1st ed. Academic Press, London: 452pp.
  11. Khan, M.A.; Iqbal, Z.; Sarwar, M.; Nisa, M.; Khan, M.S.; Lee, H.J.; Lee, W.S.; Kim, H.S. & Ki, K.S. (2006). Urea treated corncobs ensiled with or without additives for buffaloes: Ruminal characteristics, digestibility and nitrogen metabolism. Asian-Aust. J. Anim. Sci., 19(5): 705-712.
  12. Kung, J.L.; Robinson, J.R. & Pesek, J.D. (2000). Microbial populations, fermentat- ion end products and aerobic stability of corn silage treated with ammonia or a propionic acid based preservative. J. Dairy. Sci., 83: 1479-1486.
  13. Lazcano C.; Gomez-Brandon, M. & Doming- uez, J. (2008). Comparison of the effectiveness of composting and vermin- composting for the biological stabilization of cattle manure. Chemosphere, 72(7): 1013-1019.
  14. Levital, T.; Mustafaa, A.F.; Seguinb, P. & Lefebvrec, G. (2009). Effects of a propionic acid-based additive on short- term ensiling characteristics of whole plant maize and on dairy cow performance. Anim. Feed Sci. Tech., 152: 21-32.
  15. Mart?nez-Avalos, A.M.M.; Mendoza, G.D.; Cobos, M.A.; Gonzalez, S.; Garc?a-Bojalil, C.M. & Barcena, R. (1998). Nutritional evaluation of cattle manure silage with molasses for ruminants. Anim. Feed Sci. Technol., 70(3): 257-264.
  16. McDonald, P.; Hunderson, A.R. & Heron, S.J.E. (1991). The Biochemistry of Silage, 2nd ed. Cambrian Printers Lt. Aberystwyth: 340pp.
  17. Nasiru, A.; Ibrahim, M.H. & Ismail, N. (2014). Nitrogen losses in ruminant manure management and use of cattle manure vermicast to improve forage quality. Int. J. Recy. Org. Waste Agric. 3(57): 1-7.
  18. Ni, K.; Wang, Y.; Pang, H. & Cai, Y. (2014). Effect of cellulase and lactic acid bacteria on fermentation quality and chemical composition of wheat straw silage. Am. J. Plant Sci., 5(13): 1877-1884.
  19. Nisa, M.; Touqir, N.A.; Sarwar, M.; Ajmal Khan, M. & Akhtar, M. (2005). Effect of additives and fermentation periods on chemical composition and In situ digestion kinetics of Mott grass (Pennisetum purpureum) Silage. Asian-Aust. J. Anim. Sci., 18(6): 812- 815.
  20. Ogunjobi, A.A.; Ibekwe, A.C.; Babayemi, O.J. & Fagade, O.E. (2010). Microbiological evaluation of ensiled guinea grass and albizia saman pod mixtures and its effect on rumen bacterial population using in vitro fermentation technique. Asian-Aust. J.T., 13(4): 223- 232.
  21. Saeed, A.A.; AlSultani, H.M.H. & Mottaleb, A.S. (2017). Effect of addition of different levels of baker's yeast Saccharomyces cerevisiae on fermentation quality and nutritive value of ensiled whole yellow corn crop residuals. Euphrates J. Agric. Sci., 9(1): 8-18.
  22. Sarwar, M.; Khan, M.A. & Iqbal, Z. (2002). Feed resources for livestock in Pakistan. Int. J. Agric. Biol., 4: 186-192.
  23. Sarwar, M.; Nisa, M.; Hassan, Z. & Shahzad, M.A. (2006). Influence of urea molasses treated wheat straw fermented with cattle manure on chemical composition and feeding value for growing buffalo calves. Livestock Sci., 105: 151-161.
  24. Sarwar, M.; Shahzad, M.; Nisa, M.; Afzal, D.; Sharif, M. & Saddiqi, H. (2011) Feeding value of urea molasses-treated wheat straw ensiled with fresh cattle manure for growing crossbred cattle calves. Trop. Anim. Health Prod., 43(3): 543-548.
  25. SAS (2010). SAS/STAT User’s Guide for Personal Computers. Release6.12.SAS. Institute Inc., Cary, NC, USA.
  26. Scudamore, K.A. & Livesey, C.T. (1998). Occurrence and significance of mycotoxins in forage corps and silage: A review. J. Sci. Food and Agric., 77(1): 1-17.
  27. Sood, R. (1987). Medical Laboratory Technology (Methods and Interpretations). Jaypee Brothers. Medical Publishers. 2nd Ed., Emca House. 1033pp.
  28. Tilley, J.M. & Terry, R.A. (1963). A two stage technique for in vitro digestion of forage crops. J. Br. Grassland Sci., 18(2): 104-111.
  29. Weinberg, Z.G. & Muck, R.E. (1996). New trends and opportunities in the develop- ment and use of inoculants for silage. FEMS Microbiol. Rev., 19(1): 53-68.
  30. Wu, J.J.; Du, R.P.; Gao, M.; Sui, Y.Q.; Xiu, L. & Wang, X. (2014). Naturally occurring lactic acid bacteria isolated from tomatopo- mace silage. Asian-Aust. J. Anim. Sci., .27(5): 648-657.