Article Sidebar

Download
PDF
Statistic
Read Counter : 275 Download : 129

Downloads

Download data is not yet available.

Main Article Content

Abstract

This study was conducted at Dr. Taleb A. Jaayad of Molecular Genetics, Department of Animal Production, College of Agriculture, University of Basrah. Samples of fresh and canned meat of cattle, buffalo, sheep, goats, chicken and turkey were collected randomly from different areas of Basrah province, as well as blood samples of camel. The aim was to determine different animal species from their meat (except camel). DNA was extracted from meat tissue (0.2 gm) and blood by using DNA kit (Invetrogen). DNA purity was estimated by using wavelength (260-280), to be 1.8- 2.0 ng. PCR was used to amplify mtco1 gene using a general primer and gave a band of 710 bp for all species used in this study. Different species were determined by using Taq restricted enzyme. Cattle, buffalo, chicken and turkey showed one band of 637 bp. Taq enzyme has recognized sheep and goat, while sheep did not show any band to the fragment 710 bp. However, goat showed a band at 650 bp. Furthermore, camel produced two bands of 303 and 403 bp.

Keywords

Animal species meat mt CO1 PCR-RFLP

Article Details

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

How to Cite
Ayied, A. Y., & Al-Mossawi, Z. R. (2017). Determination of Different Species of Animal from their Meats by Using PCR-RFLP Technique of Mitochondria Gene COI. Basrah Journal of Agricultural Sciences, 30(1), 59–64. https://doi.org/10.37077/25200860.2017.22
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX

References

  1. Abdel-Rahman, S.M.; Elmaghraby, A.M. & Haggag, A.S. (2015). Identification and differentiation among Chicken’s, Duck’s, Quail’s, Rabbit’s and Turkey’s meat using PCR-RFLP technique. Biotech. Anim. Husb., 31(1): 101-108.
  2. Ali, M.E.; Kashif, M.; Kamal, U.; Hashim, U.; Mustafa, S. & Man, Y.B.C. (2011). Species authentication methods in foods and feeds: The present, past, and future of halal forensics. Food Analytic. Methods, 5: 935-955.
  3. Asahida, T.; Kokayashi, T.O.; Saitoh,K.O. & Nakayama, I. (1996). Tissue preservation and total DNA extraction from fish store at ambient temperature using buffers containing high concentration of urea. Fisheries Science, 62(5): 727-730.
  4. Bottero, M.T. & Dalmasso, A. (2011). Animal species identification in food products: Evolution of biomolecular methods. The Veterinary Journal, 190(1): 34-38.
  5. Botti, S. & Giuffra, E. (2010). Oligonucleotide indexing of DNA barcodes: identification of tuna and other scombrid species in food products. BMC Biotechnology, 10, (60): 1-7.
  6. Farag, M.R.; Imam, T.S. & Dhama, K. (2015). Identification of some domestic animal species (camel, buffalo and sheep) by PCR-RFLP analysis of the mitochondrial cytochrome b gene. Advances in Animal and Veterinary Sciences, 3(2): 136-142.
  7. Folmer, O.; Black, M.; Hoeh, W.; Lutz, R. & Vrijenhoek, R. (1994). DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3: 294- 299.
  8. Ford, C.S.; Ayres, K.L.; Toomey, N.; Haider, N.; van Alphen Stahl, J.; Kelly, L.J.; Wikstrom, N.; Hollingsworth, P.M.; Duff, R.J.; Hoot, S.B.; Cowan, R.S.; Chase, M.W.; Wilkinson, M.J. (2009). Selection of candidate coding DNA barcoding regions for use on land plants. Botanical Journal of the Linnean Society, 159: 1-11.
  9. Haider, N.; Nabulsi, I. & Al-Safadi, I. (2012). Identification of meat species by PCR-RFLP of the mitochondrial COI gene. Meat Science, 90: 490-493.
  10. Hebert, P.D.N.; Cywinska, A.; Ball, S.L. & deWaard, J.R. (2003a). Biological identifications through DNA barcodes. Proceedings of the Royal Society of London-Series B: Biol. Sci., 270: 313-322.
  11. Hebert, P.D.N.; Ratnasingham, S. & deWaard, J.R. (2003b). Barcoding animal life: Cytochrome c oxidase subunit 1 divergences among closely related species. Proceedings of the Royal Society London. Series B: Biol. Sci., 270: S96-S99.
  12. Kumar, A.; Kumar, R.R.; Sharma, B.D.; Mendiratta, S.K.; Gokulakrishnan, P.; Kumar, D. & Sharma, D. (2014). Authentication of goat (Capra hircus) meat using PCR amplification of mitochondrial cytochrome b gene. Small Rumin. Res., 131: 17-20.
  13. Pereira, F.; Meirinhos, J.; Amorim, A. & Pereira, L. (2006). Analysis of interspecific mitochondrial DNA diversity for accurate species identification. International Congress Series, 1288: 103-105.
  14. Spink, J. & Moyer, D.C. (2011). Defining the public health threat of food fraud. J. Food Sci., 76(9): 157-163.
  15. Sambrook, J., Fritsch, E.F. & Maniatis, T. (1989). Molecular Cloning: A laboratory manual. Cold Spring Harbor Lab. Press, Plainview, N.Y. 2nd ed., 1626pp.
  16. Stoeckle, M. (2003). Taxonomy, DNA, & the Bar Code of Life. J. Biosci.