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Abstract

The present study was undertaken to characterize the genetic diversity of the aromatase cytochrome P450 (CYP19) gene in 34 cows (15 local, 14 Holstein, and 5 Crosses) in Iraq. The objectives of the present study are to detect SNPs (mutations) in promoter p1.1 of the CYP19 gene in cattle bred in Iraq using sequencing techniques. We identified five single-nucleotide polymorphisms (SNP) loci of the CYP19 gene that were detected, namely G933T, G994C, A1044G, A1062T, and C1468A. The results showed the presence of 3, 4, and 2 polymorphic sites leading to the construction of 4, 5, and 3 different haplotypes for Holstein, local, and crosses respectively. Haplotype diversity were 0.791, 0.752, and 0.700 respectively. While nucleotide diversity was 0.0017, 0.0022, and 0.0013 respectively. Besides, we carried out a phylogenetic analysis of these sequences to address the evolutionary relationship between the animal species. These fragments were assigned in the GenBank database under the accession numbers: LC490756, LC490757, LC491437, LC491438, LC491439, LC491588, and LC491589.

Keywords

CYP19 gene Iraqi cattle single nucleotide polymorphism Genetic Diversity Phylogenetic tree

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Faraj, S. H. ., Ayied, A. Y. ., & Al-Rishdy, K. A. H. . (2020). Single Nucleotide Polymorphisms in the Promoter of CYP19 Gene in Cattle Bred in Iraq. Basrah Journal of Agricultural Sciences, 33(1), 89–97. https://doi.org/10.37077/25200860.2020.33.1.07
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