Article Sidebar

Download
PDF
Statistic
Read Counter : 58 Download : 31

Downloads

Download data is not yet available.

Main Article Content

Abstract

Since Mulberries (Morus)is a tree species with a considerable plant variety. Molecular techniques are methods used to distinguish between species accurately, easily and quickly. This study examines a Molecular method for distinguishing different Morus species in the Duhok - Kurdistan region/ Iraq. The method is based on the use of four techniques: matK gene, the ITS region, PCR-RFLP, and SRAP markers. Twelve Morus species have been selected for this study from different region of Duhok. The ITS region's PCR result was 700 bp, but the matK gene's PCR produce was 900 bp. The same restriction site was found for all utilized cultivars when the 700bp of ITS fragment was used for PCR-RFLP with two restriction enzymes, RsaI GT/AC and HaeIII GG/CC. This study also used six combinations of SRAP markers to aid in grouping and identifying genetic similarities. The results of PCR-RFLP demonstrated an insufficient link between Morus physical appearance and genetic traits, but differences across studied cultivars could be identified using SRAP markers. Furthermore, this study demonstrated the possibility of DNA barcoding Morus cultivars, as well as additional sequence analysis and the identification of probable SNP between cultivars. 

Keywords

DNA barcoding ITS region Morus matK gene PCR- RFLP SRAP markers

Article Details

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

How to Cite
Sinjare, D. Y. K. . (2024). Molecular Identification of Morus ssp. in Duhok Using Nuclear ITS Region and Chloroplast Matk Gene. Basrah Journal of Agricultural Sciences, 37(1), 86–93. https://doi.org/10.37077/25200860.2024.37.1.07
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX

References

  1. Al-Janabi, A. S., & Alhasnawi, A. N. (2021). Determination of genetic diversity based on RAPD molecular marker and ParARF3 gene expressions in some apricot genotypes in Iraq. Basrah Journal of Agricultural Sciences, 34(2), 60-74.
  2. https://doi.org/10.37077/25200860.2021.34.2.05
  3. Hu, D., Zhang, P., Hao, W., Sun, Y., Wang, Z., & Chen, C. (2015). SRAP analysis on the genetic relationships of 24 mulberry (Morus L.) accessions in the lower regions of the Yellow River. Genetic resources and crop evolution, 62, 13-19.
  4. https://doi.org/10.1007/s10722-014-0215-1
  5. Hussein, A. E., & Jubrael, J. M. (2021). AFLP marker in genetic diversity assessment of fig (Ficus carica L.) populations in kurdistan region – Iraq. The Iraqi Journal of Agricultural Science, 52(4), 859-867. https://doi.org/10.36103/ijas.v52i4.1393
  6. İpek, M., Pirlak, L., & Kafkas, S. (2012). Molecular characterization of mulberry (Morus spp.) genotypes via RAPD and ISSR. Journal of the Science of Food and Agriculture, 92(8), 1633-1637
  7. https://doi.org/10.1002/jsfa.4749
  8. Kadam, R. A., Dhumal, N. D., & Khyade, V. B. (2019). The Mulberry, Morus alba L.: The medicinal herbal source for human health. International Journal of Current Microbiology and Applied Sciences, 8(4), 2941-2964.
  9. https://doi.org/10.20546/ijcmas.2019.804.341
  10. Kosman, E., & Leonard, K. J. (2005). Similarity coefficients for molecular markers in studies of genetic relationships between individuals for haploid, diploid, and polyploid species. Molecular ecology, 14(2), 415-424.
  11. https://doi.org/10.1111/j.1365-294X.2005.02416.x
  12. Mondal, T. K., (2020). Molecular markers. Pp, 139-194. In Mondal, T. K. (Editor). Tea: Genome and Genetics, Springer Singapore, 312pp.
  13. https://doi.org/10.1007/978-981-15-8868-6
  14. Mosa, K. A., Gairola, S., Jamdade, R., El-Keblawy, A., Al Shaer, K. I., Al Harthi, E. K., & Mahmoud, T. (2019). The promise of molecular and genomic techniques for biodiversity research and DNA barcoding of the Arabian Peninsula flora. Frontiers in Plant Science, 9, 1929.
  15. https://doi.org/10.3389/fpls.2018.01929
  16. Nei, M., & Li, W. H. (1979). Mathematical model for studying genetic variation in terms of restriction endonucleases. Proceedings of the National Academy of Sciences, 76(10), 5269-5273.
  17. https://doi.org/10.1073/pnas.76.10.5269
  18. Nepal, M. P., & Ferguson, C. J. (2012). Phylogenetics of Morus (Moraceae) inferred from ITS and trnL-trnF sequence data. Systematic Botany, 37(2), 442-450. https://doi.org/10.1600/036364412X635485
  19. Ouborg, N. J., Pertoldi, C., Loeschcke, V., Bijlsma, R. K., & Hedrick, P. W. (2010). Conservation genetics in transition to conservation genomics. Trends in Genetics, 26(4), 177-187.
  20. https://doi.org/10.1016/j.tig.2010.01.001
  21. Sexton, J. P., Hangartner, S. B., & Hoffmann, A. A. (2014). Genetic isolation by environment or distance: which pattern of gene flow is most common?. Evolution, 68(1), 1-15.
  22. https://doi.org/10.1111/evo.12258
  23. Salih, K., Yasar, A., & Sezai, E. (2022). Analysis of genetic relationships of mulberry (Morus L.) genotypes using inter-simple sequence repeats (ISSR) markers. Erwerbs-Obstbau, 64(1), 75-83. https://doi.org/10.1007/s10341-021-00614-1
  24. Yan, L., Tianbao, L., Cankui, Z., & Zhiqiang, L. (2023). Proteomics in mulberry. Pp, 167-182. In: Gnanesh, B. N., & Vijayan, K. (Editors). The Mulberry Genome, Springer, Cham. 298pp.
  25. https://doi.org/10.1007/978-3-031-28478-6_7
  26. Yuan, Q., & Zhao, L. (2017). The mulberry (Morus alba L.) Fruit a review of characteristic components and health benefits. Journal of Agricultural and Food Chemistry, 65(48), 10383-10394.
  27. https://doi.org/10.1021/acs.jafc.7b03614
  28. Zeng, Q., Chen, H., Zhang, C., Han, M., Li, T., Qi, X., Xiang, Z., & He, N. (2015). Definition of eight mulberry species in the genus Morus by internal transcribed spacer-based phylogeny. PloS one, 10(8), e0135411.
  29. https://doi.org/10.1371/journal.pone.0135411
  30. Zhang, S. D., Soltis, D. E., Yang, Y., Li, D. Z., & Yi, T. S. (2011). Multi-gene analysis provides a well-supported phylogeny of Rosales. Molecular Phylogenetics and Evolution, 60(1), 21-28.
  31. https://doi.org/10.1016/j.ympev.2011.04.008
  32. Zhao, W., Fang, R., Pan, Y., Yang, Y., Chung, J., Chung, I., & Park, Y. (2009). Analysis of genetic relationships of mulberry (Morus L.) germplasm using sequence-related amplified polymorphism (SRAP) markers. African Journal of Biotechnology, 8(11).
  33. https://www.ajol.info/index.php/ajb/article/view/60782