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Abstract
Squash (Cucurbita pepo) fields in Mosul suffered from virus-like symptoms, such as vein clearing, severe mosaic, mottling, chlorosis, necrosis, stunting and fruit discoloration. The symptoms were associated with aphids presence in the infected fields. A metatranscriptomic analysis has been applied to detect the causal agent. The result showed that 559,024 mapped reads were related to Cucurbit aphid-borne yellows virus with full coverage to produce 5,662 bp consensus sequence. The virus sequence was deposited in National Center for Biotechnology Information (NCBI) under accession number OQ865059, and the isolate named Iraq. For the first time in Iraq, the infection of the virus has been reported in squash, highlighting the impact of such virus infection that spread epidemically across the squash and other cucurbits fields.
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References
- Kearse, M., Moir, R., Wilson, A., Stones-Havas, S., Cheung, M., Sturrock, S., Buxton, S., Cooper, A., Markowitz, S., Duran, C. and Thierer, T., (2012). Geneious Basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics, 28(12), 1647-1649. https://doi.org/10.1093/bioinformatics/bts199
- Menzel, W., Maeritz, U., & Seigner, L. (2020). First report of Cucurbit aphid-borne yellows virus infecting cucurbits in Germany. New Disease Reports, 41(1), 2044-0588. http://doi.org/10.5197/j.2044-588.2020.041.001
- Lecoq, H., Bourdin, D., WIPF‐SCHEIBEL, C., Bon, M., Lot, H., Lemaire, O., & Herrbach, E. (1992). A new yellowing disease of cucurbits caused by a luteovirus, cucurbit aphid‐borne yellows virus. Plant Pathology, 41(6), 749-761.https://doi.org/10.1111/j.1365-3059.1992.tb02559.x
References
Kearse, M., Moir, R., Wilson, A., Stones-Havas, S., Cheung, M., Sturrock, S., Buxton, S., Cooper, A., Markowitz, S., Duran, C. and Thierer, T., (2012). Geneious Basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics, 28(12), 1647-1649. https://doi.org/10.1093/bioinformatics/bts199
Menzel, W., Maeritz, U., & Seigner, L. (2020). First report of Cucurbit aphid-borne yellows virus infecting cucurbits in Germany. New Disease Reports, 41(1), 2044-0588. http://doi.org/10.5197/j.2044-588.2020.041.001
Lecoq, H., Bourdin, D., WIPF‐SCHEIBEL, C., Bon, M., Lot, H., Lemaire, O., & Herrbach, E. (1992). A new yellowing disease of cucurbits caused by a luteovirus, cucurbit aphid‐borne yellows virus. Plant Pathology, 41(6), 749-761.https://doi.org/10.1111/j.1365-3059.1992.tb02559.x