Main Article Content
Abstract
An experiment for strawberry Fragaria ananassa cv. Festival in vitro conservation of cultures for nine months was studied. Three MS medium strength (full strength MS, ¾ MS, ½ MS, and ¼ MS) and the storage period (3, 6, and 9 months) in slowing down or reducing growth. Results showed a significant effect of the medium salt strength, as the full strength treatment was higher by achieved the highest percentage of viability (survival %) and storage characteristics of cultures (N%, P%, carbohydrates%, protein %) and regeneration ability characteristics (shoots and roots number and length). Three-month storage period achieved the highest rate compared to the nine-month storage period, which recorded the lowest average for the studied characteristics. The full strength treatment for three months storage period realized the highest rate compared to treatment ¼ strength for the storage period of nine months, which recorded the lowest rate of the studied traits. The application of minimal strength for in vitro conservation allows strawberry cultures to remain viable with a slow growth rate and a significant increase in the storage life of the tissues to nine months.
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References
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- Al- Rawi, K. M., & Khalaf Allah A. A. M. (2000). Design and Analysis of Agricultural Experiments. Books House for Printing and Publishing. University of Mosul. 484 pp. (in Arabic).
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- Samarina, L. S., Choudhary, R., Kolomiets, T. M., Abilfazova, Y. S., & Saran, P. L. (2014). In vitro Conservation Technique for Russian Citrus limon. Agricultural Research, 3, 279-283. https://www.tib.eu/en/search/id/BLSE%3ARN363052056
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References
Ahmed, M., Anjum, M. A., Shah, A.H., & Hamid, A. (2010). In vitro conservation of Pyrus germ¬plasm with minimal growth using different temperature regimes. Pakistan Journal of Botany, 42, 1639-1650. http://www.pakbs.org/pjbot/PDFs/42(3)/PJB42(3)1639.pdf
Al- Rawi, K. M., & Khalaf Allah A. A. M. (2000). Design and Analysis of Agricultural Experiments. Books House for Printing and Publishing. University of Mosul. 484 pp. (in Arabic).
Dubois, M. K., Crilles, K. A., Hamiltor, J. K., Rebers, D. A., & Smith, F. (1956). Colorimetric method for determination of sugars and substance. Analytical Chemistry, 28, 350-365. https://doi.org/10.1021/ac60111a017
FAO, (2016). Food and Agriculture Organization of the United Nations. FAOSTAT agriculture data. http://faostat3.fao.org/download/Q/QC/E. Accessed May 2016.
Franco, S., (2009). The Production of Strawberries in Iraq. Ministry of Agriculture, Iraq. pp. 1-10. U.S. Agency for International development (USAID).
Huang, H. P., Wang, J., Huang, L. Q., Gao, S. L., Huang, P., & Wang, D. L. (2014). Germplasm preservation in vitro of Polygonum multiflorum Thunb. Pharmacognosy Magazine, 10, 179–184. http://www.phcog.com/text.asp?2014/10/38/179/131083
Husaini, A. M., & Neri, D. (2016). Strawberry: Growth, development and diseases. CABI Publishing, 315pp. https://doi.org/10.1079/9781780646633.0000
Kamińska, M., Skrzypek, E., Wilmowicz, E. E., Tretyn, A., & Trejgell, A. (2016). Effect of light conditions and ABA on cold storage and post-storage propagation of Taraxacum pieninicum. Plant Cell, Tissue and Organ Culture, 127, 25-34. https://doi.org/10.1007/s11240-016-1026-1
Kalra, Y. P., (1998). Handbook of Reference Methods for Plant Analysis. Soil and Plant Analysis Council, Inc., CRC Press. FL., 287pp. https://plantstress.com/wp-content/uploads/2020/03/Handbook_of_Reference_Methods_for_Plant_Analysis-1998.pdf
Lowry, O. H., Rosebrough, N. J., Farr, A. L., & Randull, R. J. (1951). Protein measurement with Folin phenol reagent. Journal of Biological Chemistry, 193, 295-297. https://www.jbc.org/article/S0021-9258(19)52451-6/pdf
Maryam, A., Tariq, A., Chuadhary, S., Azmat, R., Javed, S., & Khanam, S. (2014). A review: Role of tissue culture (in vitro) techniques in the conservation of rare and endangered species. Pacific Journal of Life Sciences, 2, 93-103. https://www.academia.edu/7259757
Mohammed, M. H. (2019). Response of strawberry plant Fragaria ananassa Duch to light quality and some growth stimulators in vitro. M. Sc. Thesis. Faculty of Agriculture, University of Kufa, 111pp. (in Arabic).
Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15, 473- 497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Reed, B. M. (1992). A cold storage of strawberries in vitro: A comparison of three storage systems. Fruit Varieties Journal, 46, 98–102. https://europepmc.org/article/agr/ind92031926
Rokosa, M., & Mikiciuk, M. (2017). In vitro regeneration of Fragraria plants. Acta Scientiarum Polonorum, Hortorum Cultus, 16, 145–158. http://www.hortorumcultus.actapol.net/volume16/issue5/16_5_145.pdf
Samarina, L. S., Choudhary, R., Kolomiets, T. M., Abilfazova, Y. S., & Saran, P. L. (2014). In vitro Conservation Technique for Russian Citrus limon. Agricultural Research, 3, 279-283. https://www.tib.eu/en/search/id/BLSE%3ARN363052056
Sota, V., & Kongjika, E. (2014). Slow growth in vitro conservation of Zizyphus jujuba Mill. Agriculture & Forestry, 60, 27-37. http://89.188.43.75/agricultforest/20140218-03%20SOTA%20and%20KONGJIKA.pdf.
Sota, V., & Kongjika, E. (2019). Micropropagation and in vitro conservation via slow growth techniques of Prunus webbii (Spach) Vierh: An endangered plant species in Albania. International Journal of Agricultural and Biosystems Engineering, 13, 11, 280-283. https://doi.org/10.5281/zenodo.3566425.
Trejgell, A., Kaminska, M., & Tretyn, A. (2015). In vitro slow growth storage of Senecio macrophyllus shoots. Acta Physiologiae Plantarum, 37, 234. https://doi.org/10.1007/s11738-015-1983-8.
Vinay, S. & Afroz, A. (2015). Plant Tissue Culture. I K. International Publishing House. 378pp. https://www.waterstones.com/book/plant-tissue-culture/vinay-sharma/afroz-alam/9789384588441
VSN International. (2019). GenStat for Windows 12th Edition. VSN International, Hemel Hempstead, UK.