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Abstract
In order to investigate the possibility for in in vitro fast regeneration of Dianthus caryophyllus, different concentrations of Kinetin (Kin), 2,4-Dichlorophenoxy Acetic Acid (2,4-D) had been tested for induction of callus by leaves and stems explant using Murashige and Skoog (MS) medium. After two months in culture, response callus in the induction rate, fresh and dry weight, texture and color were evaluated. MS medium that contained 4.5 mg.L-1 2, 4-D was suitable for callus induction with leaf explants. The combination of 0.4 mg.L-1 Kin and 2.0 mg.L-1 2, 4-D also demonstrated a wonderful induction of callus on stem explants. In addition to the culture medium complemented by Kin and 2, 4-D, the influence of Zeatin at various concentrations (0.0, 0.4, 0.8 or 1.0 mg.L-1) was assessed. In MS medium that contained 0.4 mg.L-1 Kin+ 2.0 mg.L-1 2,4-D+ 1.0 mg.L-1 Zeatin, rapid callus induction and also more callus proliferation from stem explants were detected. Results indicate on callus induction that, Zeatin was more successful than Kin or 2, 4-D, but other combinations directly developed a shoot. Callus obtained from stems explant was friable, white and yellow, while callus from leaf explants was green.
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References
- Reference
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- Kanwar, J. K., & Kumar, S. S. (2010). Effect of growth regulators, explants and their interaction on shoot regeneration in Carnation. Advances in Horticultural Science, 24, 115-121. https://doi.org/10.17221/3750-HORTSCI
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- Maurya, R., Sharma, M., Yadav, M. K., Kumar, G., & Kumar, M. (2020). In Vitro high-frequency callus induction in carnation (Dianthus caryophyllus L.) cultivar “IRENE” Plant Cell Biotechnology and Molecular Biology, 20, 1363–1368. file:///C:/Users/huda/AppData/Local/Temp/Paper178
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- Shiba, T., & Mll, M. (2005). Plant regeneration from mesophyll- and cell suspension-derived protoplasts of Dianthus acicularis and characterization of regenerated plants. In vitro Cellular and Developmental Biology –Plant, 41, 794-800. https://doi.org/10.1079/ivp2005712
References
Reference
Al-Asadi, A. Z., Abdulwahid, A. H., & Al-Mayahi, A. M. W. (2019). The effect of thidiazuron on callus and in vitro shoots development of Date Palm (Phoenix dactylifera L.) cv. Barhee. Basrah Journal of Agricultural Sciences, 32, 258-265. https://bjas.bajas.edu.iq/index.php/bjas/article/view/90/82
Al-Birawee, A. R., & Nasser, A. K. (2019). Gel extraction from caper fruits (Capparies spinosa L.) and assess its effectiveness as antioxidants. Basrah Journal Agricultural Sciences, 32, 74-84. https://bjas.bajas.edu.iq/index.php/bjas/article/view/111/98
Al-Snafi, A. S. (2017). Chemical contents and medical importance of Dianthus caryophyllus- A review. IOSR Journal of Pharmacy, 7, 61-71. www.iosrjournals.org
Chandra, S., Rawat, D. S., Chandra, D., & Rastogi, J. (2016). Nativity, phytochemistry, ethno botany and pharmacology of Dianththus caryophyllus. Research Journal of Medical Plant, 10, 1-9. http://docsdrive.com/pdfs/academicjournals/rjmp/2016/1-9.
Chandran, H., Meena, M., Barupal, T., & Sharma, K. (2020). Plant tissue culture as a perpetual source for production of industrially important bioactive compounds. Biotechnology Reports Journal, 20, 6:e00450.https://doi.org/10.1016/j.btre.2020.e00450.
Espinosa-Leal, C. A., Puente-Garza, C. A., & García-Lara, S. (2018). In vitro plant tissue culture: means for production of biological active compounds. Planta, 248, 1–18. https://doi.org/10.1007/s00425-018-2910-1
Grunennvaldt, R. L., Degenhardt-Goldbach, J., Brooks, P., Tomasi, F. A. H., Tran, T., Gomes, E. N., & Deschamps, C. (2020). Callus culture as a new approach for the production of high added value compounds in Ilex paraguariensis: Genotype influence, medium optimization and compounds identification. Academia Brasileira de Ciências. 92, http://www.alice.cnptia..br/alice/handle/doc/1126527
Jorapur, S., Jogdande, N., & Dhumale, D. (2018). Petal callus mediated de novo regeneration of shoots in carnation (Dianthus caryophyllus L.). The Pharma Innovation Journal, 7, 218-222. https://www.thepharmajournal.com/archives/2018/vol7issue1/PartD/7-1-38-894
Kanwar, J. K., & Kumar, S. S. (2010). Effect of growth regulators, explants and their interaction on shoot regeneration in Carnation. Advances in Horticultural Science, 24, 115-121. https://doi.org/10.17221/3750-HORTSCI
Kaur, K. (2014). In vitro mass cloning at Dinthus chinesis. A horticulture important plant. Dissertation reported, Department of Biotechnology, Thapar University. http://hdl.handle.net/10266/3120
Madhuri, G., & Barad, A. V. (2018). Flowering parameters of carnation (Dianthus caryophyllus L.) varieties under protected condition influenced by NPK nutrients through foliar spray. The Pharma Innovation Journal, 7, 105-108. https://www.thepharmajournal.com/archives/2018/vol7issue8/PartB/7-5-177-645.pdf
Mahood, H. E., Alwash, B. J., & Ibrahim, K. M. (2018). Improvement of alkaloids yield using phenylalanine as a precursor supplemented to Morina oleifera L. callus cultures. Biochemical and Cellular Archives, 18, 913-919. http://www.connectjournals.com/achivestoc2.php?fulltext=2866300H_913-919.pdf&&bookmark=CJ-033216&&issue_id=Supplement&&yaer=2018M
Maurya, R., Sharma, M., Yadav, M. K., Kumar, G., & Kumar, M. (2020). In Vitro high-frequency callus induction in carnation (Dianthus caryophyllus L.) cultivar “IRENE” Plant Cell Biotechnology and Molecular Biology, 20, 1363–1368. file:///C:/Users/huda/AppData/Local/Temp/Paper178
Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue culture. Plant Physiology, 15, 473-497. https://onlinelibrary.wiley.com/doi/epdf/10.1111/j.1399-3054.1962.tb08052.x
SAS. (2012). Statistical Analysis System, User's Guide. Statistical. Version 9.1th ed. SAS. Inst. Inc. Cary. N. C. USA. https://support.sas.com/documentation/onlinedoc/stat/indexchapter.html
Shiba, T., & Mll, M. (2005). Plant regeneration from mesophyll- and cell suspension-derived protoplasts of Dianthus acicularis and characterization of regenerated plants. In vitro Cellular and Developmental Biology –Plant, 41, 794-800. https://doi.org/10.1079/ivp2005712