Main Article Content
Abstract
The present study aims to identify and characterize the chemical compounds present in the extracts of cactus pear Opuntia dillenii (OD) fruit which grow in southern Iraq and offer different health benefits. The physicochemical properties of the fruit juices (pulp and peels) were investigated. Fruit pulp and peel extracts carried the symbols: hexane pulp extract (HPuE), hexane peel extract (HPeE), ethanolic pulp extract (EPuE), and ethanolic peels extract (EPeE). The activel compound screened by gas chromatography-mass spectrometry (GC-MS). HPuE and HPeE contained 40 bioactive compounds and 60 bioactive compounds were detected in EPuE and EPeE. The scavenging activity of DPPH free radicals records a high level of inhibition ranging from 34.8 to 90.3% for EPeE and from 32.9 to 89.9% for EPuE, while hexane extracts scavenging activities (HPeE and HPuE) were 24.9-78.9% and 20.7-73.3% respectively at concentrations of 20 – 80 µg.ml-1 this activity has received considerable attention because of their physiological functions
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References
- Aghajani, Z., Ali-Asghar, E., & Akhbari M. (2015). Identification of volatile components and biological properties of the extracts of aerial parts of Pterocarya fraxinifolia L. Biological Forum – An International Journal, 7, 262-266. https://www.researchtrend.net/bfij/bf12/44%20ZAHRA%20AGHAJANI.pdf
- Albano, C., Miceli, A., Negro, C., Tommasi, N., Gerardi, C., Mita G., Bellis, L.D., & Blando., F. (2015). Betalains, phenols, and antioxidant capacity in cactus Pear [Opuntia ficus-indica (L.) Mill.] fruits from Apulia (South Italy) genotypes. Antioxidant, 4, 269-280. https://doi.org/10.3390/antiox4020269
- Alsaad, A. J., Altemimi, A. B., Aziz,S. N., & Lakhssassi, N. (2019). Extraction and identification of Cactus (Opuntia dillenii) seed oil and its added value for human health benefits. Pharmacogn Journal, 11, 1-9. https://doi.org/10.5530/PJ.2019.3
- Anwar, M. M., & Sallam, E. M. (2016). Utilisation of Prickly Pear Peels to Improve Quality of Pan Bread. Arab Journal of Nuclear Science and Applications, 49, 151-163. https://inis.iaea.org/search/search.aspx?orig_q=journal:%22ISSN%201110-0451%22
- AOAC (2010). Official Methods of Analysis of the Association of Official Analytical Chemists. 18th Ed. Published by the Association of Official Analytical Chemists. Arlington, Virginia. 2220pp. https://www.techstreet.com/standards/official-methods-of-analysis-of-aoac-international-18th-edition-revision-3?product_id=1678986
- Bakari, S., Daoud, A., Felhi,S., Smaoui, S., Gharsallah, N., & Kadri, A. (2017). Proximate analysis, mineral composition, phytochemical contents, antioxidant and antimicrobial activities and GC-MS investigation of various solvent extracts of cactus cladode. Food Science and Technology, 37, 286-293. http://dx.doi.org/10.1590/1678-457X.20116
- Bergaoui, A., Boughalleb, N., Ben Jannet, H., HarzallahShiric, F., El Mahjoub, M., & Mighri, Z. (2007). Chemical composition and antifungal activity of volatiles from three Opuntia species growing in Tunisia. Pakistan Journal of Biological Sciences, 10, 2485-2489. http://dx.doi.org/10.3923/pjbs.2007.2485.2489
- Bouzoubaâ, Z., Essoukrati, Y., Tahrouch, S., Hatimi, A., Gharby, S., & Harhar, H. (2016). Phytochemical study of prickly pear from southern Morocco. Journal of Saudi Society of Agricultural Sciences, 15, 155-161. https://doi.org/10.1016/j.jssas.2014.09.002 (In Turkish)
- Butera, D., Tesoriere, L., Di Gaudio, F., Bongiorno, A., Allegra, M., Pintaudi, A. M., Kohen, R., & Livrea, M. A. (2002). Antioxidant activities of Sicilian prickly pears (Opuntia ficus-indica) fruit extracts and reducing properties of its betalains: betanin and indicaxanthin. Journal of Agricultural and Food Chemistry, 50, 6895–6901. https://doi.org/10.1021/jf025696p
- Buyukokuroglu, M. E., Gulcin, I., Oktay, M., & Kufrevioglu, O. I. (2001). In vitro antioxidant properties of dantrolene sodium). Pharmacological Research, 44, 491-4. https://doi.org/10.1006/phrs.2001.0890
- Duru, B., & Turker, N. (2005). Changes in physical properties and chemical composition of cactus pear (Opuntia ficus-indica) Duringmaturation. Journal of the Professional Association for Cactus Development, 7, 22-33.
- Faten, M. A. & Ali, R. F. M. (2014). Antioxidant and anticancer activities of different constituents extracted from Egyptianprickly pear cactus (Opuntia ficus-Indica) peel. Biochemistry and Analytical Biochemistry, 3, 2-8. https://doi.org/10.4172/2161-1009.1000158
- Fernández-López, J. A., Almela, L., Obón, J. M., & Castellar, R. (2010). Determination of antioxidant constituents in cactus pear fruits. Plant Foods for Human Nutrition, 65, 253–259. https://doi.org/10.1007/s11130-010-0189-x
- Flath, R. A. & Takahashi, J. M. (1978). Volatile constituents of prickly Pear (Opuntia ficus indica Mill.(de CastillaVariety). Journal of Agricultural and Food Chemistry, 835-837. https://doi.org/10.1021/jf60218a053
- Gulcin, I., Buyukokuroglu, M. E., Oktay, M., & Kufrevioglu, O. I. (2003). Antioxidant and analgesic activities of turpentine of Pinus nigra Arn. subsp. pallsiana (Lamb.) Holmboe. Journal of Ethnopharmacology, 86, 51-58. https://doi.org/10.1016/s0378-8741(03)00036-9
- Gurrieri, S., Miceli, L., Lanza, C. M., Tomaselli, F., Bonomo, R. P., & Rizzarelli, E. (2000). Chemical scharacterisation of Sicilian prickly pear (Opuntia ficus indica) and perspectives for the storage of its juice. Journal of Agricultural and Food Chemistry, 48, 5424–5431. https://doi.org/10.1021/jf9907844
- Jacobo, M. C. (2001). Cactus pear breeding and domestication. Plant Breeding Reviews, 20, 135–166. https://doi.org/10.1002/9780470650189.ch5
- Jaiswal, D., Rai, P. K., & Watal, G. (2009). Antidiabetic effect of Withania coagulants in the experimental rat. Indian Journal of Clinical Biochemistry, 24, 88-93.https://doi.org/10.1007/s12291-009-0015-0
- Koubaa, M., Ktata, A., Bouaziz, F., Driss, D., Ghorbel, R. E. & Chaabouni, S. E. (2015). Solvent extract from Opuntia stricta fruit peels Chemical composition and biological activities. Free Radicals & Antioxidants, 5, 52-59.https://doi.org/10.5530/fra.2015.2.2
- Madrigal-Santillán, E., García-Melo, F., Morales-González, J. A., Vázquez-Alvarado, P., Muñoz-Juárez, S., Zuñiga-Pérez, C., Sumaya-Martínez, M. T., Madrigal-Buiaidar, E., & Hernández-Ceruelos, A. (2013). The antioxidant and anticlastogenic capacity of prickly pear juice. Nutrients, 5, 4145– 4158. https://doi.org/10.3390/nu5104145
- Makris, D. P., Boskou, G., & Andrikopoulos, N. K. (2007). Polyphenolic content and in vitro antioxidant characteristics of the wine industry and other agri-food solid waste extracts, Journal of Food Composition and Analysis, 20, 125–132. https://doi.org/10.1016/j.jfca.2006.04.010
- Matias, A., Nunes, S. L., Poejo, J., Mecha, E., Serra, A. T., Amorim Madeira, P. J., Bronze, M. R., & Duarte, C. M. M. (2014). Antioxidant and anti-inflammatory activity of a flavonoid-rich concentrate recovered from Opuntia ficus-indica juice. Food & Function, 5, 3269–3280. https://doi.org/10.1039/c4fo00071d
- Moßhammer, M. R., Stintzing, F. C., & Carle, R. (2006). Cactus Pear fruits (Opuntia spp.): A review of processing technologies and current uses. Journal of the Professional Association for Cactus Development, 8, 1-25. https://www.researchgate.net/publication/279652903
- Morales, J., & ´Enez-Perez, S. J. (2001). Free radical scavenging capacity of Maillard reaction products as related to colour and fluorescence. Food Chemistry, 72, 119–125. https://doi.org/10.1016/S0308-8146(00)00239-9
- Osorio-Esquivel, O., Ortiz-Moreno, A., Álvarez, V. B., Dorantes-Álvarez, L., & Giusti, M. M. (2011). Phenolics, betacyanins) and antioxidant activity in Opuntia joconostle fruits. Food Research International, 44, 2160 –2168. https://doi.org/10.1016/j.foodres.2011.02.011
- Paixao, N., Perestrelo, R., Marques, J. C., & Camara, J. S. (2007). Relationship between antioxidant capacity and total phenolic content of red rose and white wines. Food Chemistry, 105, 204–214. https://doi.org/10.1016/j.foodchem.2007.04.017
- Parish, J., & Felker, P. (1997). Fruit quality and production of Cactus pear (Opuntia spp.). Fruit clones selected for increased frost hardiness, Journal of Arid Environments, 37, 123-143. https://doi.org/10.1006/jare.1997.0261
- Piga, A. (2004). Cactus pear: A fruit of nutraceutical and functional importance. Journal Professional Association for Cactus Development, 6, 9-22.
- Prior, R. L., Wu, X., & Schaich, K. (2005). Standardised methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. Journal of Agricultural and Food Chemistry, 53, 4290–4302. https://doi.org/10.1021/jf0502698
- Rajeswari, G., Murugan, M., & Mohan, V. R. (2012). GC-MS analysis of bioactive components of Hugonia mystax L. (Linaceae). Research Journal of Pharmaceutical, Biological, and Chemical Sciences, 3, 301-318.
- Ramadan, M. F., & Mörsel, J. T. (2003). Oil Cactus pear (Opuntia ficus-indica L.). Food Chemistry, 82, 339–345. https://doi.org/10.1016/S0308-8146(02)00550-2
- Sáenz, C. (2000). Processing technologies: an alternative for Cactus pear (Opuntia spp.) fruits and cladodes. Journal of Arid Environments, 46, 209-225. https://doi.org/10.1006/jare.2000.0676. (In Turkish).
- Sarkadei, S., & Howell, N. K. (2007). Effect of natural antioxidants on stored freeze-dried food product formulated using horse mackerel (Trachurus trachurus). Journal of Science and Technology, 43, 309-315. https://doi.org/10.1111/j.1365-2621.2006.01435.x
- Shahidi, F., Janitha, P. K., & Wanasundara, P. D. (1992). Phenolic antioxidants. Critical Reviews in Food Science and Nutrition, 32, 67-103. https://doi.org/10.1080/10408399209527581
- Sharma, C., Rani, S., Kumar, B., Kumar, A., & Raj, V. (2015). Plant Opuntia dillenii: A review on its traditional uses, phytochemical, and pharmacological properties. EC Pharmaceutical Science, 11, 29-43. https://www.semanticscholar.org/paper/Plant-Opuntia-dillenii%3A-A-Review-on-It%27s-Uses%2C-and-Sharma-Rani/e7cdd1e6ac4dc6ef2e6b4987d84e39327eb6bac9
- Valente, L. M. M., daPaixão, D., do Nascimento, A. C., dos Santos, P. F. P., Scheinvar, L. A., Moura, M. R. L., Tinoco, L. W., Gomes, L. N. F., & da Silva, J. F. M. (2010). Antiradical activity, nutritional potential, and flavonoids of the cladodes of Opuntia monacantha (Cactaceae). Food Chemistry, 123, 1127-1131. https://doi.org/10.1016/j.foodchem.2010.05.074
- Yadav, L., & Chakravarty, A. (2013). Effect of keeping time period on acidityof fruit juices and determination of fungal growth in fruit juices. Asian Journal of Home Science, 8, 166-169. https://www.semanticscholar.org/paper/Effect-of-Keeping-Time-Period-on-Acidity-of-Fruit-Yadav-Chakravarty/89a25fb7beb9a2505ef226ab0b2082ade9e6cd66
- Zhang, W., Li, B., Han, L., & Zhang, H. (2009). Antioxidant activities of extracts from (Areca catechu L.) flower husk and seed. African Journal of Biotechnology, 8, 3887-3892. https://www.ajol.info/index.php/ajb/index
- Zito, P., Sajeva, M., Bruno, M., Rosselli, S., Maggio, A., & Senatore, F. (2013). Essential oils composition of two Sicilian cultivars of Opuntia ficus-indica (L.) Mill (Cactaceae) fruits (prickly pear). Natural Product Research, 27, 1305- 1314. https://doi.org/10.1080/14786419.2012.734823.
References
Aghajani, Z., Ali-Asghar, E., & Akhbari M. (2015). Identification of volatile components and biological properties of the extracts of aerial parts of Pterocarya fraxinifolia L. Biological Forum – An International Journal, 7, 262-266. https://www.researchtrend.net/bfij/bf12/44%20ZAHRA%20AGHAJANI.pdf
Albano, C., Miceli, A., Negro, C., Tommasi, N., Gerardi, C., Mita G., Bellis, L.D., & Blando., F. (2015). Betalains, phenols, and antioxidant capacity in cactus Pear [Opuntia ficus-indica (L.) Mill.] fruits from Apulia (South Italy) genotypes. Antioxidant, 4, 269-280. https://doi.org/10.3390/antiox4020269
Alsaad, A. J., Altemimi, A. B., Aziz,S. N., & Lakhssassi, N. (2019). Extraction and identification of Cactus (Opuntia dillenii) seed oil and its added value for human health benefits. Pharmacogn Journal, 11, 1-9. https://doi.org/10.5530/PJ.2019.3
Anwar, M. M., & Sallam, E. M. (2016). Utilisation of Prickly Pear Peels to Improve Quality of Pan Bread. Arab Journal of Nuclear Science and Applications, 49, 151-163. https://inis.iaea.org/search/search.aspx?orig_q=journal:%22ISSN%201110-0451%22
AOAC (2010). Official Methods of Analysis of the Association of Official Analytical Chemists. 18th Ed. Published by the Association of Official Analytical Chemists. Arlington, Virginia. 2220pp. https://www.techstreet.com/standards/official-methods-of-analysis-of-aoac-international-18th-edition-revision-3?product_id=1678986
Bakari, S., Daoud, A., Felhi,S., Smaoui, S., Gharsallah, N., & Kadri, A. (2017). Proximate analysis, mineral composition, phytochemical contents, antioxidant and antimicrobial activities and GC-MS investigation of various solvent extracts of cactus cladode. Food Science and Technology, 37, 286-293. http://dx.doi.org/10.1590/1678-457X.20116
Bergaoui, A., Boughalleb, N., Ben Jannet, H., HarzallahShiric, F., El Mahjoub, M., & Mighri, Z. (2007). Chemical composition and antifungal activity of volatiles from three Opuntia species growing in Tunisia. Pakistan Journal of Biological Sciences, 10, 2485-2489. http://dx.doi.org/10.3923/pjbs.2007.2485.2489
Bouzoubaâ, Z., Essoukrati, Y., Tahrouch, S., Hatimi, A., Gharby, S., & Harhar, H. (2016). Phytochemical study of prickly pear from southern Morocco. Journal of Saudi Society of Agricultural Sciences, 15, 155-161. https://doi.org/10.1016/j.jssas.2014.09.002 (In Turkish)
Butera, D., Tesoriere, L., Di Gaudio, F., Bongiorno, A., Allegra, M., Pintaudi, A. M., Kohen, R., & Livrea, M. A. (2002). Antioxidant activities of Sicilian prickly pears (Opuntia ficus-indica) fruit extracts and reducing properties of its betalains: betanin and indicaxanthin. Journal of Agricultural and Food Chemistry, 50, 6895–6901. https://doi.org/10.1021/jf025696p
Buyukokuroglu, M. E., Gulcin, I., Oktay, M., & Kufrevioglu, O. I. (2001). In vitro antioxidant properties of dantrolene sodium). Pharmacological Research, 44, 491-4. https://doi.org/10.1006/phrs.2001.0890
Duru, B., & Turker, N. (2005). Changes in physical properties and chemical composition of cactus pear (Opuntia ficus-indica) Duringmaturation. Journal of the Professional Association for Cactus Development, 7, 22-33.
Faten, M. A. & Ali, R. F. M. (2014). Antioxidant and anticancer activities of different constituents extracted from Egyptianprickly pear cactus (Opuntia ficus-Indica) peel. Biochemistry and Analytical Biochemistry, 3, 2-8. https://doi.org/10.4172/2161-1009.1000158
Fernández-López, J. A., Almela, L., Obón, J. M., & Castellar, R. (2010). Determination of antioxidant constituents in cactus pear fruits. Plant Foods for Human Nutrition, 65, 253–259. https://doi.org/10.1007/s11130-010-0189-x
Flath, R. A. & Takahashi, J. M. (1978). Volatile constituents of prickly Pear (Opuntia ficus indica Mill.(de CastillaVariety). Journal of Agricultural and Food Chemistry, 835-837. https://doi.org/10.1021/jf60218a053
Gulcin, I., Buyukokuroglu, M. E., Oktay, M., & Kufrevioglu, O. I. (2003). Antioxidant and analgesic activities of turpentine of Pinus nigra Arn. subsp. pallsiana (Lamb.) Holmboe. Journal of Ethnopharmacology, 86, 51-58. https://doi.org/10.1016/s0378-8741(03)00036-9
Gurrieri, S., Miceli, L., Lanza, C. M., Tomaselli, F., Bonomo, R. P., & Rizzarelli, E. (2000). Chemical scharacterisation of Sicilian prickly pear (Opuntia ficus indica) and perspectives for the storage of its juice. Journal of Agricultural and Food Chemistry, 48, 5424–5431. https://doi.org/10.1021/jf9907844
Jacobo, M. C. (2001). Cactus pear breeding and domestication. Plant Breeding Reviews, 20, 135–166. https://doi.org/10.1002/9780470650189.ch5
Jaiswal, D., Rai, P. K., & Watal, G. (2009). Antidiabetic effect of Withania coagulants in the experimental rat. Indian Journal of Clinical Biochemistry, 24, 88-93.https://doi.org/10.1007/s12291-009-0015-0
Koubaa, M., Ktata, A., Bouaziz, F., Driss, D., Ghorbel, R. E. & Chaabouni, S. E. (2015). Solvent extract from Opuntia stricta fruit peels Chemical composition and biological activities. Free Radicals & Antioxidants, 5, 52-59.https://doi.org/10.5530/fra.2015.2.2
Madrigal-Santillán, E., García-Melo, F., Morales-González, J. A., Vázquez-Alvarado, P., Muñoz-Juárez, S., Zuñiga-Pérez, C., Sumaya-Martínez, M. T., Madrigal-Buiaidar, E., & Hernández-Ceruelos, A. (2013). The antioxidant and anticlastogenic capacity of prickly pear juice. Nutrients, 5, 4145– 4158. https://doi.org/10.3390/nu5104145
Makris, D. P., Boskou, G., & Andrikopoulos, N. K. (2007). Polyphenolic content and in vitro antioxidant characteristics of the wine industry and other agri-food solid waste extracts, Journal of Food Composition and Analysis, 20, 125–132. https://doi.org/10.1016/j.jfca.2006.04.010
Matias, A., Nunes, S. L., Poejo, J., Mecha, E., Serra, A. T., Amorim Madeira, P. J., Bronze, M. R., & Duarte, C. M. M. (2014). Antioxidant and anti-inflammatory activity of a flavonoid-rich concentrate recovered from Opuntia ficus-indica juice. Food & Function, 5, 3269–3280. https://doi.org/10.1039/c4fo00071d
Moßhammer, M. R., Stintzing, F. C., & Carle, R. (2006). Cactus Pear fruits (Opuntia spp.): A review of processing technologies and current uses. Journal of the Professional Association for Cactus Development, 8, 1-25. https://www.researchgate.net/publication/279652903
Morales, J., & ´Enez-Perez, S. J. (2001). Free radical scavenging capacity of Maillard reaction products as related to colour and fluorescence. Food Chemistry, 72, 119–125. https://doi.org/10.1016/S0308-8146(00)00239-9
Osorio-Esquivel, O., Ortiz-Moreno, A., Álvarez, V. B., Dorantes-Álvarez, L., & Giusti, M. M. (2011). Phenolics, betacyanins) and antioxidant activity in Opuntia joconostle fruits. Food Research International, 44, 2160 –2168. https://doi.org/10.1016/j.foodres.2011.02.011
Paixao, N., Perestrelo, R., Marques, J. C., & Camara, J. S. (2007). Relationship between antioxidant capacity and total phenolic content of red rose and white wines. Food Chemistry, 105, 204–214. https://doi.org/10.1016/j.foodchem.2007.04.017
Parish, J., & Felker, P. (1997). Fruit quality and production of Cactus pear (Opuntia spp.). Fruit clones selected for increased frost hardiness, Journal of Arid Environments, 37, 123-143. https://doi.org/10.1006/jare.1997.0261
Piga, A. (2004). Cactus pear: A fruit of nutraceutical and functional importance. Journal Professional Association for Cactus Development, 6, 9-22.
Prior, R. L., Wu, X., & Schaich, K. (2005). Standardised methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. Journal of Agricultural and Food Chemistry, 53, 4290–4302. https://doi.org/10.1021/jf0502698
Rajeswari, G., Murugan, M., & Mohan, V. R. (2012). GC-MS analysis of bioactive components of Hugonia mystax L. (Linaceae). Research Journal of Pharmaceutical, Biological, and Chemical Sciences, 3, 301-318.
Ramadan, M. F., & Mörsel, J. T. (2003). Oil Cactus pear (Opuntia ficus-indica L.). Food Chemistry, 82, 339–345. https://doi.org/10.1016/S0308-8146(02)00550-2
Sáenz, C. (2000). Processing technologies: an alternative for Cactus pear (Opuntia spp.) fruits and cladodes. Journal of Arid Environments, 46, 209-225. https://doi.org/10.1006/jare.2000.0676. (In Turkish).
Sarkadei, S., & Howell, N. K. (2007). Effect of natural antioxidants on stored freeze-dried food product formulated using horse mackerel (Trachurus trachurus). Journal of Science and Technology, 43, 309-315. https://doi.org/10.1111/j.1365-2621.2006.01435.x
Shahidi, F., Janitha, P. K., & Wanasundara, P. D. (1992). Phenolic antioxidants. Critical Reviews in Food Science and Nutrition, 32, 67-103. https://doi.org/10.1080/10408399209527581
Sharma, C., Rani, S., Kumar, B., Kumar, A., & Raj, V. (2015). Plant Opuntia dillenii: A review on its traditional uses, phytochemical, and pharmacological properties. EC Pharmaceutical Science, 11, 29-43. https://www.semanticscholar.org/paper/Plant-Opuntia-dillenii%3A-A-Review-on-It%27s-Uses%2C-and-Sharma-Rani/e7cdd1e6ac4dc6ef2e6b4987d84e39327eb6bac9
Valente, L. M. M., daPaixão, D., do Nascimento, A. C., dos Santos, P. F. P., Scheinvar, L. A., Moura, M. R. L., Tinoco, L. W., Gomes, L. N. F., & da Silva, J. F. M. (2010). Antiradical activity, nutritional potential, and flavonoids of the cladodes of Opuntia monacantha (Cactaceae). Food Chemistry, 123, 1127-1131. https://doi.org/10.1016/j.foodchem.2010.05.074
Yadav, L., & Chakravarty, A. (2013). Effect of keeping time period on acidityof fruit juices and determination of fungal growth in fruit juices. Asian Journal of Home Science, 8, 166-169. https://www.semanticscholar.org/paper/Effect-of-Keeping-Time-Period-on-Acidity-of-Fruit-Yadav-Chakravarty/89a25fb7beb9a2505ef226ab0b2082ade9e6cd66
Zhang, W., Li, B., Han, L., & Zhang, H. (2009). Antioxidant activities of extracts from (Areca catechu L.) flower husk and seed. African Journal of Biotechnology, 8, 3887-3892. https://www.ajol.info/index.php/ajb/index
Zito, P., Sajeva, M., Bruno, M., Rosselli, S., Maggio, A., & Senatore, F. (2013). Essential oils composition of two Sicilian cultivars of Opuntia ficus-indica (L.) Mill (Cactaceae) fruits (prickly pear). Natural Product Research, 27, 1305- 1314. https://doi.org/10.1080/14786419.2012.734823.