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The study aimed to evaluate the effect of dietary supplements on ruminal digestion and intestinal microbiocenosis of lactating cows. The study was carried out under production conditions on three groups of new black-and-white heifers (10 heads each). Mean live weight of cows was 500±20 kg. The cows of the control group received a basic diet, which included haylage of perennial grasses, corn silage, legume hay, concentrate feed, and molasses. Cows of the experimental groups, in addition to the basal diet (BD), received the Farmatan TM supplement (consists of a balanced combination of tannins, essential oils of clove and cinnamon, sodium acetate, and organic zinc) at a dose of 40 g. head-1 per day (experimental group 1) and a pine tree energy supplement at a dose of 150 g. head-1 per day (experimental group 2). The supplement was mixed with concentrate feed and given once in the morning feeding. In the course of the study, the parameters of ruminal fermentation in experimental animals and the microorganisms of the rumen and the large intestine were studied. At the same time, it was found that the inclusion of the Farmatan TM feed additive (40 g per day) and pine tree energy supplement (150 g per day) in the diet of cows at the beginning of lactation contributed to the strengthening of enzymatic processes in the rumen, which was expressed in an increase in the formation of volatile fatty acids by 7.6 and 20.3%, an increase in the proportion of propionic acid and a slight decrease in the proportion of acetic and butyric acids. When using feed additives (Farmatan TM and pine tree energy supplement), a decrease in the content of Clostridium in the rumen of cows was noted by 26.3 and 30.3% in the 1st and 2nd experimental groups, respectively, compared with the control group. In cows of the experimental groups, a decrease in the content of molds and yeast-like fungi in the rumen was also noted. The results obtained allow recommending the studied additives (Farmatan TM and pine tree energy supplement) to enhance enzymatic processes in the rumen.


Clostridium Dietary supplement Microbiocenosis Ruminal digestion

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Zaitsev, V. V. ., Bogolyubova, N. V. ., Zaitseva, L. M. ., Emelyanova, I. S. ., Korotkiy, V. P. ., & Ryzhov, V. A. . (2022). Effect of Dietary Supplements Farmatan TM and Pine Tree Energy on the Regulation of Ruminal Digestion and Microbiocenosis of Lactating Black and White Cows. Basrah Journal of Agricultural Sciences, 35(2), 99–109.


  1. Al-Khafaji, S.A., Alsaadawi, M.A., Al-Yasari, A.M., & Al-Saadawe, M.A. (2021). Article review: Cordia myxa L.: The gift of the nature, A Review. Basrah Journal of Agricultural Sciences, 34(2), 267-277.
  3. Appolloni E., Pennisi, G., Zauli, I., Carotti, L., Paucek, I., Quaini, S., Orsini, F., & Gianquinto, G. (2021). Beyond vegetables: Effects of indoor LED light on specialized metabolite biosynthesis in medicinal and aromatic plants, edible flowers, and microgreens. Journal of The Science of Food and Agriculture, 102(2), 472-487.
  5. Bogolyubova, N.V., Zaytsev, V.V., & Shalamova, S.A. (2018). Methods of regulating physiological and biochemical processes and improving performance of dairy cows summer period. Research Journal of Pharmaceutical, Biological, and Chemical Sciences, 9(4), 1390-1395.
  6. Bonelli, F., Turini, L, Sarri, G, Serra, A, Buccioni, A, & Mele, M. (2018). Oral administration of chestnut tannins to reduce the duration of neonatal calf diarrhea. BMC Veterinary Research, 14(1), 227.
  8. Chiba, L. (2014). Rumen microbiology and fermentation. In Chiba, L. (Ed.). Animal Nutrition Handbook. Auburn, AL: Self-published, p. 57-79.
  9. Council of Europe. (1986). The European Convention for the Protection of Vertebrate Animals used for Experimental and other Scientific Purposes. European Treaty Series No. 123. Strasbourg, March 18, 1986.
  11. De Nardi, R., Marchesini, G., Li, S., Khafipour, E., Plaizier, K.J.C., Gianesella, M., Ricci, R., Andrighetto, I., & Segato, S. (2016). Metagenomic analysis of rumen microbial population in dairy heifers fed a high grain diet supplemented with dicarboxylic acids or polyphenols. BMC Veterinary Research, 12, 29.
  13. Girard, M., Dohme-Meier, F., Wechsler, D., Goy, D., Kreuzer, M., & Bee, G. (2016). Ability of 3 tanniferous forage legumes to modify quality of milk and Gruyère-type cheese. Journal of Dairy Science, 99, 205-220.
  15. Harahap, R.P., Suharti, S., Ridla, M., Laconi, E.B., Nahrowi, N., Irawan, A., Kondo, M., Obitsu, T., & Jayanegara, A. (2022). Meta-analysis of dietary chitosan effects on performance, nutrient utilization, and product characteristics of ruminants. Animal Science Journal, 93(1), e1367.
  17. Hu, Q., Zhou, M., & Wei, S. (2018). Progress on the Antimicrobial Activity Research of Clove Oil and Eugenol in the Food Antisepsis Field. Journal of Food Science, 83(6), 1476-1483.
  19. Huws, S. A., Creevey, C. J., Oyama, L. B., Mizrahi, I., Denman, S. E., Popova, M., Muñoz-Tamayo, R., Forano, E., Waters, S. M., Hess, M., Tapio, I., Smidt, H., Krizsan, S. J., Yáñez-Ruiz, D. R., Belanche, A., Guan, L., Gruninger, R. J., McAllister, T. A., Newbold, C. J., Roehe, R., Dewhurst, R. J., Snelling, T. J., Watson, M., Suen, G., Hart, E. H., Kingston-Smith, A.H., Scollan, N.D., do Prado, R. M., Pilau, E. J., Mantovani, H. C., Attwood, G. T., Edwards, J. E., McEwan, N. R., Morrisson, S., Mayorga, O. L., Elliott, C., & Morgavi, D. P. (2018). Addressing global ruminant agricultural challenges through understanding the rumen microbiome: Past, Present, and Future. Frontiers in Microbiology, 9, 2161.
  21. Klebaniuk, R., Kochman, G., Kowalczuk-Vasilev, E., Grela, E.R., Bąkowski, M., Olcha, M., & Dunster, F. (2016). Energy efficiency of diet for periparturient dairy cows supplemented with free fatty acids or glucogenic additives. Medycyna weterynaryjna, 72(12), 760-767.
  23. Kondrakhin, I. P. (Ed.). (2004). Metody veterinarnoi klinicheskoi laboratornoi diagnostiki: spravochnik [Methods of veterinary clinical laboratory diagnostics: A reference book]. Kolos, Moscow: 520pp. (In Russian).
  25. Liu, J., Li, H., Zhu, W., & Mao, S. (2019). Dynamic changes in rumen fermentation and bacterial community following rumen fluid transplantation in a sheep model of rumen acidosis: implications for rumen health in ruminants. The FASEB Journal, 33(7), 8453-8467.
  27. Mackie, R. I., McSweeney, C. S., & Aminov, R. I. (2013). Rumen. eLS. John Wiley & Sons, Ltd: Chichester 13pp.
  29. Min, B. R., & Solaiman, S. (2018). Comparativeaspects of plant tannins on digestive physiology, nutrition andmicrobial community changes in sheep and goats: A review. Journal of Animal Physiology and Animal Nutrition, 102, 1181-1193.
  31. Mousavi, A., Hossein Mahdavi, A., Riasi, A., & Soltani-Ghombavani, M. (2018). Efficacy of essential oils combination on performance, ileal bacterial counts, intestinal histology and immunocompetence of laying hens fed alternative lipid sources. Journal of Animal Physiology and Animal Nutrition, 102(5), 1245-1256.
  33. Oomah, D. B. (2020). Flaxseed By-products. 267-289. In Campos-Vega, R., Oomah, B. D., & Vergara-Castañeda, H. A. (Eds.). Food Wastes and By‐products: Nutraceutical and Health Potential. John Wiley & Sons Ltd. 480pp.
  35. Orton, T., Rohn, K., Breves, G., & Bred, M. (2020). Alterations in fermentation parameters during and after induction of a subacute rumen acidosis in the rumen simulation technique. Journal of Animal Physiology and Animal Nutrition, 104(6), 1678-1689.
  37. Plyashchenko, S. I., & Sidorov, V. T. (1979). Natural resistance of the organism of animals. Kolos. 183s.
  38. Váradyová, Z., Mravčáková, D., Holodová, M., Grešáková, Ľ., Pisarčíková, J., Barszcz, M., Taciak, M., Tuśnio, A., Kišidayová, S., & Čobanová, K. (2018). Modulation of ruminal and intestinal fermentation by medicinal plants and zinc from different sources. Journal of Animal Physiology and Animal Nutrition, 102(5), 1131-1145.
  40. Xiao, Y., Xiang, Y., Zhou, W., Chen, J., Li, K., & Yang, H. (2017). Microbial community mapping in intestinal tract of broiler chicken. Poultry Science, 96(5), 1387-1393.
  42. Yıldızlı, G., Coral, G., & Ayaz, F. (2021). Biochar as a Biocompatible Mild Anti-Inflammatory Supplement for Animal Feed and Agricultural Fields. Chemistry & Biodiversity, 18(6), e2001002.
  44. Yu, C., Zhang, M., Liu, J., Zhang, J., Xu, J., & Xu, W. (2022). Effects of sodium acetate on lipid metabolism, antioxidant capability and cell apoptosis of blunt snout bream (Megalobrama amblycephala) hepatocytes treated by sodium palmitate. Aquaculture Research, 53(3), 1098-1109.
  46. Zhang, T., Yang, Y., Liang, Y., Jiao, X., & Zhao, C. (2018). Beneficial effect of intestinal fermentation of natural polysaccharides. Nutrients, 10(8), 1055.

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