Article Sidebar

Download
PDF
Statistic
Read Counter : 288 Download : 213

Downloads

Download data is not yet available.

Main Article Content

Abstract

The study was conducted in the Microbiology Laboratory of the Department of Food Science at Basrah University's, Faculty of Agriculture from 15/11/2020 to 26/12/2020, to study the flora of lactic acid bacteria in digestive parts )Jejunum, Ileum, and Ceca (for healthy adult chickens and identifying the target part that contains the largest number of lactic acid bacteria for different types of poultry. As a result, as well as isolating bacterial colonies after three times, and being genetically Identified with PCR, seven new species of lactic acid bacteria were acquired and registered at the National Center for Biotechnology Information (NCBI) as new local strains, both in Iraq and around the world, as follows: Lactobacillus gasseri strain Al-Salhi-1, Lactobacillus helveticus strain Al-Salhi-2, Lactiplantibacillus plantarum strain Al-Salhi-3, Limosilactobacillus reuteri strain AhQuSa-1, Limosilactobacillus sp. strain AhQuSa-2, Ligilactobacillus salivarius strain AhQuSa-3, Lactobacillus Johnsonii strain AhQuSa-4. The results also showed a significant superiority (P≤0.05) in the logarithmic numbers of lactic acid bacteria in the jejunum for each of : the group of birds bred in local environments, as well as in the group of birds bred in commercial fields in comparison with the Ileum and Ceca, as the bacterial content was in the group of local birds : 6.52, 5.21, 4.15 (Cfu/g) for each of the jejunum, ileum and Ceca, respectively, while in the group of commercial birds it reached: 6.35, 5.02, 3.92 (Cfu/g) for each of the jejunum, ileum and Ceca, respectively.

Keywords

Intestinal microflora Lactobacillus Microbial Count Poultry Strains

Article Details

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

How to Cite
Al-Salhi, A. A. ., Al-Shatty, S. M. ., Al-Imara, E. A. ., & Al-Khfaji, Q. J. . (2022). A New Record of Lactic Acid Bacteria Strains from the Contents of Adult Chicken Intestines. Basrah Journal of Agricultural Sciences, 35(2), 199–222. https://doi.org/10.37077/25200860.2022.35.2.14
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX

References

  1. Akalu, N., Assefa, F., & Dessalegn, A. (2017). In vitro evaluation of lactic acid bacteria isolated from traditional fermented Shamita and Kocho for their desirable characteristics as probiotics. African Journal of Biotechnology, 16(12), 594-606
  2. Cappuccino, J. G., & Welsh, C. T. (2019). Microbiology: A laboratory manual. 12 th edition, Pearson publisher, New York. 541pp.
  3. Da Silva, N., Taniwaki, M. H., Junqueira, V. C. A., Silveira, N., Okazaki, M. M., & Gomes, R. A. R. (2019). Microbiological examination methods of food and water: A laboratory manual. 2nd edn. Routledge, Taylor and Francis Group, London, 526pp.
  4. Dec, M., Nowaczek, A., Urban-Chmiel, R., Stępień-Pyśniak, D., & Wernicki, A. (2018). Probiotic potential of Lactobacillus isolates of chicken origin with anti-Campylobacter activity. Journal of Veterinary Medical Science, 80(8), 1195-1203.
  5. https://doi.org/10.1292/jvms.18-0092
  6. Ghazal, M. M., Al-Hilfy, N. A. A., & Ali, H. I. (2021). Production of functional soft cheese and studying its chemical and sensory evaluation properties. Basrah Journal of Agricultural Sciences, 34(1), 67-83.‏
  7. https://doi.org/10.37077/25200860.2021.34.1.07
  8. Holt, J. G. (2004). Bergey's manual of determinative bacteriology. 9th edn. Baltimore, Williams & Wilkins, 787pp.
  9. Jain, A., Jain, R., & Jain, S. (2020). Preservation of microorganisms: Stabs, slants, lyophilization and cryopreservation. Pp, 105-110. In: Jain, A., Jain, R., & Jain, S. (Eds.). Basic techniques in biochemistry, microbiology, and molecular biology: Principles and Techniques, Springer Protocols Handbooks. Humana, New York, 282pp.
  10. https://doi.org/10.1007/978-1-4939-9861-6_30
  11. Jha, R., Das, R., Oak, S., & Mishra, P. (2020). Probiotics (direct-fed microbials) in poultry nutrition and their effects on nutrient utilization, growth and laying performance, and gut health: A Systematic Review. Animals, 10(10), 1863.
  12. https://doi.org/10.3390/ani10101863
  13. Johnson, T. R., & Case, C. L. (2019). Laboratory experiments in microbiology. 12th edition. Pearson Education, Inc., New York, 516pp.
  14. Markowiak, P., & Śliżewska, K. (2018). The role of probiotics, prebiotics, and synbiotics in animal nutrition. Gut Pathogens, 10, 21
  15. https://doi.org/10.1186/s13099-018-0250-0
  16. Mohamed, F. M., Thabet, M. H., & Ali, M. F. (2019). The use of probiotics to enhance the immunity of broiler chicken against some intestinal infection pathogens. SVU-International Journal of Veterinary Sciences, 2(1), 1-19.
  17. https://doi.org/10.21608/svu.2019.23141
  18. Nasser, E. K., Majeed, K. R., & Ali, H. I. (2021). Effect of some strains of lactic acid bacteria and their mixture on the level of fats and cholesterol in albino rats (Rattus norvegicus) male with hypothyroidism induced using carbimazole. Basrah Journal of Agricultural Sciences, 34(1), 139-146.‏
  19. https://doi.org/10.37077/25200860.2021.34.1.12
  20. Procop, G. W., Church, D. L., Hall, G. S., Janda, W. M., Koneman, E.W, Schreckenberger, P. C., & Woods, G. L. (2017). Koneman's color atlas and textbook of diagnostic microbiology.7th edn., ‏Lippincott Williams & Wilkins, Baltimore. Publishers. Philadelphia: Wolters Kluwer Health, 2733pp.
  21. http://library.lol/main/F9564B3A82DA094CA51BDDC04328B675
  22. Sambrook, J., Fritsch, E., & Maniatis T. (2001). Molecular cloning a laboratory manual. 4th edn., Cold Spring Harbor Laboratory Press, New York, 2222pp.
  23. http://library.lol/main/08ED769A253765B81383D10063642E47
  24. Shang, Y., Kumar, S., Oakley, B., & Kim, W. K. (2018). Chicken gut microbiota: Importance and detection technology. Frontiers in Veterinary Science, 5.
  25. https://doi.org/10.3389/fvets.2018.00254
  26. Sjofjan, O., & Adli, D. N. (2020). Effect of dietary supplementation mannan-riched fraction (mrf) and probiotic-enhanced liquid acidifier on the growth performance, serum blood biochemistry, and intestinal properties of broilers. In: IOP Conference Series: Earth and Environmental Science, 478(1), 012066). IOP Publishing.
  27. https://doi.org/10.1088/1755-1315/478/1/012066
  28. SPSS: Statistical Package for the Social Sciences (2018). SPSS users guide. Statistics, Version 25. IBM SPSS Statistics, SPSS Institute, Inc, Chicago, IL.
  29. Vieco-Saiz, N., Belguesmia, Y., Raspoet, R., Auclair, E., Padgett, C., Bailey, C., & Drider, D. (2022). Protective effects of novel Lactobacillaceae strains isolated from chicken Caeca against necrotic enteritis infection: In vitro and in vivo evidences. Microorganisms, 10(1), 152.‏
  30. https://doi.org/10.3390/microorganisms10010152
  31. Vogelstein, B., & Gillespie, D. (1979). Preparative and analytical purification of DNA from agarose. Proceedings of the National Academy of Sciences, 76(2), 615-619.‏
  32. https://doi.org/10.1073/pnas.76.2.615
  33. Vos, P., Garrity, G., Jones, D., Krieg, N. R., Ludwig, W., Rainey, F. A., & Whitman, W. B. (Eds.). (2009). Bergey's manual of systematic bacteriology: Volume 3: The Firmicutes, Vol. 3, 2nd edn., Springer Science & Business Media.
  34. http://library.lol/main/2952206F9758A3E302981BB07C386BD6
  35. Walter, J., Tannock, G. W., Tilsala-Timisjarvi, A., Rodtong, S., Loach, D. M., Munro, K., & Alatossava, T. (2000). Detection and identification of gastrointestinal Lactobacillus species using denaturing gradient gel electrophoresis and species-specific PCR primers. Applied and Environmental Microbiology, 66(1), 297-303.
  36. https://doi.org/10.1128/aem.66.1.297-303.2000
  37. Wang, J., Ishfaq, M., Guo, Y., Chen, C., & Li, J. (2020). Assessment of probiotic properties of Lactobacillus salivarius isolated from chickens as feed additives. Frontiers in Veterinary Science, 7.
  38. https://doi.org/10.3389/fvets.2020.00415