Article Sidebar

Download
PDF
Statistic
Read Counter : 234 Download : 140

Downloads

Download data is not yet available.

Main Article Content

Abstract

This study was conducted to determine the effect of Amino acid Methionine and Lysine on the reproductive performance of male Awassi sheep. Twenty Awassi males aged range 10-12 months and average weight 32± 0.5kg were used in this study, during the period from May to August 2022. The animals were randomly divided into four equal groups. The first group was the control group (T1). In this group, sheep were fed on standard diet without any additive. The second treated group (T2) were fed on standard diet supplemented with coated Methionine (1.5g/sheep/day). The third treated group (T3) were fed on standard diet supplemented with coated Lysine (2.5g/sheep/day). The fourth treated group (T4) were fed on standard diet supplemented with coated Methionine and Lysine at the same doses used in T2 and T3 .The results showed that there was a significant improvement (P≤0.05) in sperm characteristics of T4 as compared to other groups. T4 also showed the highest testosterone levels as compared with the other groups.T4group also showed a well development in the seminiferous tubules, Sertoli cells and Leydig cells as compared withT1.T4 showed the best histological testicular architecture especially Sertoli cells as compared with T1,T2andT3. It was concluded from this study that coated Methionine and Lysine might improve semen characteristics, testosterone levels and male reproductive performance in Awassi male.

Keywords

Coated lysine Coated methionine Semen Sheep Testosterone

Article Details

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

How to Cite
Agwaan, H. W. . K. . (2023). Effect of Coated Methionine and Lysine and Their Combination on Fertility of Awassi Male Sheep. Basrah Journal of Agricultural Sciences, 36(1), 201–213. https://doi.org/10.37077/25200860.2023.36.1.17
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX

References

  1. Aitken, R. J., Whiting, S., De Iuliis, G. N., McClymont, S., Mitchell, L. A., & Baker, M. A. (2012). Electrophilic aldehydes generated by spermmetabolism activate mitochondrial reactive oxygen species generation and apoptosis by targeting succinate dehydrogenase. Journal of Biological Chemistry, 287, 33048-33060.
  2. https://doi.org/10.1074/jbc.M112.366690
  3. Alahmar, A. T. (2019). Role of oxidative stress in male infertility: An updated review. Journal of Human Reproductive Sciences, 12, 4–18.
  4. https://doi org/10.4103/jhrs.JHRS_150_18
  5. Alkhashab, A. T., Al- Hassan, F. H., Al-Dabbagh, S. F., Kassim, H. W., & Abd Albaki, T. M. (2021). Effect of protected methionine supplementation on body weight, testicular parameters, semen characteristics and testosterone hormone of awassi ram lambs. Plant Archives, 21(1), 1238-1242.
  6. Al-Khawaja, A.K., Al-Bayati, I.A., & Abdul-Ahad, S. (1978). Chemical composition and nutritional value of Iraqi feed materials. Third Edition. Department of Nutrition - Ministry of Agriculture and Agrarian Reform. The Republic of Iraq.(In Arabic).
  7. AOAC International (2016) Guidelines for Standard Method Performance Requirements AOAC Official Methods of Analysis. Appendix F, 1-18.
  8. https://www.aoac.org/resources/guidelines-for-standard-method-performance-requirements/
  9. Ashton, W. S., Degnan, B. M., Danie, I. A., & Francis, G. L. (1995). Testosterone increases insulin-like growth factor-1 and insulin-like growth factor-binding protein. Annals of Clinical and Laboratory Science, 25(5), 381-388.
  10. Collin, F. (2019). Chemical basis of reactive oxygen species reactivity and involvement in neurodegenerative diseases. International Journal of Molecular Sciences, 20(10), 2407.
  11. https://doi.org/10.3390/ijms20102407
  12. Creasy, D. M. (2002). Histopathology of the Male Reproductive System I: Techniques. Current Protocols in Toxicology, 3rdEd:1-18.
  13. https://doi.org/10.1002/0471140856.tx1603s12
  14. Culling, C. F. A. (1974). Handbook of histopathological and histological techniques. 3rd ed., Butterworks-Heinemann. London, 271pp.
  15. Cupp, A. S., & Skinner, M. K. (2001). Expression, action, and regulation of transforming growth factor alpha and epidermal growth factor receptor during embryonic and perinatal rat testis. Journal of Andrology, 22(6), 1019-1029.
  16. https://doi.org/10.1002/j.1939-4640.2001.tb03443.x
  17. Dong, H-J., Wu, D., Xu, S.-Y., Li, Q., Fang, Z.-F., Che, L.-Q., Wu, C.-M., Xu, X.-Y., & Lin, Y. (2016). Effect of dietary supplementation with amino acids on boar sperm quality and fertility. Animal Reproduction Science, 172, 182-189.
  18. https://doi.org/10.1016/j.anireprosci.2016.08.003
  19. Donkin, S. S., Varga, G. A., Sweeney, T. F., & Muller, L. D. (1989). Rumen-protected methionine and lysine: effects on animal performance, milk protein yield, and physiological measures. Journal of Dairy Science, 72(6), 1484-1491.
  20. https://doi.org/10.3168/jds.S0022-0302(89)79258-4
  21. Duncan, D. (1955). Multiple Ranges and Multiple F-test. Biometrics, 11, 1- 24.
  22. El-Sharawy, M. E., Salam, A.A., Ibrah, M. A. R., & El-Shamaa, I. S. (2012). Impacts of zinc methionine and zinc oxide on enzymes and quality of ram semen. Egyptian Journal of Nutrition and Feeds, 15(1), 58-66.
  23. Ezzat, W. A. A., Hanan, S. M., & Fathey, I. A. (2019). Effect of propolis and zinc methionine supplementation on improvement of productive, reproductive and immunity performance of local insash strain under Egyptian summer condition. Egyptian Poultry Science Journal, 39(1), 253-273.
  24. https://doi.org/10.21608/epsj.2019.29841
  25. Gilbreath, K. R., Bazer, F. W., Satterfield, M. C., & Wu, G. (2021). Amino acid nutrition and reproductive performance in ruminants. Advances in experimental medicine and biology, 1285, 43-61.
  26. https://doi.org/10.1007/978-3-030-54462-1_4
  27. Hacham, Y., Song, L., Schuster, G., & Amir, R. (2007). Lysine enhances methionine content by modulating the expression of S-adenosylmethionine synthase. The Plant Journal: for Cell and Molecular Biology, 51(5), 850-861.
  28. https://doi.org/10.1111/j.1365-313X.2007.03184.x
  29. Hafez, B., & Hafez, E. S. E. (2000). Reproduction in Farm Animals. Lippincott Williams & Wilkins, Wiley Online Library.
  30. https://onlinelibrary.wiley.com/doi/book/10.1002/9781119265306
  31. Hong, J. D., De, W., Sheng, Y. X., Qiang, L., Zheng, F. F., Lian, Q. C., Cai, M. W., Xue, Y. X., & Yan, L. (2016). Effect of dietary supplementation with amino acids on boar sperm quality and fertility. Animal Reproduction Science, 172, 181-189
  32. https://doi.org/ 10.1016/j.anireprosci.2016.08.003
  33. Huang, Y. L., Zhang, P. F., Hou, Z., Fu, Q., Li, M. X., Huang, D. L., Deng, T. X., Lu, Y. Q., Liang, X. W., & Zhang, M. (2020). Ubiquitome analysis reveals the involvement of lysine ubiquitination in the spermatogenesis process of adult buffalo (Bubalus bubalis) testis. Bioscience Reports, 40(6), BSR20193537.
  34. https://doi.org/10.1042/BSR20193537
  35. Kwasek, K., Dabrowski, K., Nynca, J., Wojno, M., & Wick, M. (2014). The influence of dietary Lysine on yellow perch maturation and the quality of sperm. North American Journal of Aquaculture, 76(2), 119-126.
  36. https://doi.org/10.1080/15222055.2013.856826
  37. Lahnsteiner, F. (2010). A comparative study on the composition and importance of free amino acids in semen of gilthead sea bream, Sparus aurata, and perch, Perca fluviatilis. Fish physiology and biochemistry, 36(4), 1297–1305.
  38. https://doi.org/10.1007/s10695-010-9442-3
  39. Mavrommatis, A., Mitsiopoulou, C., Christodoulou, C., Kariampa, P., Simoni, M., Righi, F., & Tsiplakou, E. (2021). Effects of supplementing rumen-protected methionine and lysine on milk performance and oxidative status of dairy ewes. Antioxidants, 10(5), 654.
  40. https://doi.org/10.3390/antiox10050654
  41. Mirzoyan, A. V., Nebesikhina, N. A., & Voynova, N. V. (2006). Preliminary results on ascorbic acid and lysine suppression of clastogenic effect of deep-frozen sperm of the Russian sturgeon. International Journal of Refrigeration, 29(3), 374-378.
  42. https://doi.org/10.1016/j.ijrefrig.2005.07.008
  43. Mohany, N., Totti, A., Naylor, K. R., & Janovjak H. (2021). Microbial methionine transporters and biotechnological applications. Applied microbiology and biotechnology 105(10), 3919-3929.
  44. https://doi.org/10.1007/s00253-021-11307-w
  45. Nizza, A., Di Meo, C., & Taranto, S. (2000). Effect of lysine and methionine on libido and semen characteristics of bucks. World Rabbit Science, 8(4), 181-184.
  46. https://doi.org/10.4995/wrs.2000.437
  47. Petersen, P. M. (1999). Stereologica methods as efficient and unbiased tools to quantitate structures in the testis.” Scandinavian Journal of Work, Environment & Health, 25, 31–33.
  48. http:/www.jstor.org/stable/40966972
  49. Petersen, P. M., Seierøe, K., & Pakkenberg, B. (2015). Thetotal number of leydig and sertoli cells in the testes of men across various age groups-astereological study. Journal of Anatomy, 226(2), 175-9.
  50. https://doi.org/10.1111/joa.12261
  51. Ritagliati, C., Luque, G. M., Stival, C., Baro Graf, C., Buffone M. G., & Krapf, D. (2018). Lysine acetylation modulates mouse sperm capacitation. Scientific Reports, 8(1), 13334.
  52. https://doi.org/10.1038/s41598-018-31557-5
  53. SAS. (2001). SAS/ STAT Users Guide for Personal Computers. SAS Institute. Inc. Cary, N.C.
  54. Schwab, C. G., Whitehouse, N. L., McLaughlin, A. M., Kadariya, R. K. P., Stpierre, N. R., Sloan, B. K., Gill, R. M., & Robert, J. C. (2001). Use of milk protein concentrations to estimate the methionine bioavailability of two forms of 2 -hydroxyl-4 methylthio butanoic acid (HMB) for lactating cows. Journal of Dairy Science, 84(1), 35. (Abstr.)
  55. Shamiah, Sh., Abd El-Karim, R. E., Eshera, A. A. M., Fouda, S. F., & Zaghloul, H. A. (2017). Effect of dietary selenomethionine supplementation on semen quality, fertility and antioxidant status of cockerels. Egyptian Journal of Nutrition and Feeds, 20(2), 227-236.
  56. https://ejnf.journals.ekb.eg/article_104119.html
  57. Sun, G., Jiang, M., Zhou, T., Guo, Y., Cui, Y., Guo, X., & Sha, J. (2014). Insights into the lysine acetylproteome of human sperm. Journal of Proteomics, 109, 199-211.
  58. https://doi.org/10.1016/j.jprot.2014.07.002
  59. Szczykutowicz, J., Kałuża, A., Kaźmierowska-Niemczuk, M., & Ferens Sieczkowska, M. (2019). The potential role of seminal plasma in the fertilization outcomes. BioMed Research International, ID 5397804, 10pp
  60. https://doi.org/10.1155/2019/5397804
  61. Ugur, M. R., Dinh, T., Hitit, M., Kaya, A., Topper, E., Didion, B., & Memili, E. (2020). Amino Acids of Seminal Plasma Associated with Freeze ability of Bull Sperm. Frontiers in Cell and Developmental Biology, 7, 347. https://doi.org/10.3389/fcell.2019.00347
  62. Wei, H., Zhao, X., Xia, M., Tan, C., Gao, J., Htoo, J. K., Xu, C., & Peng, J. (2019). Different dietary methionine to lysine ratios in the lactation diet: effects on the performance of sows and their offspring and methionine metabolism in lactating sows. Journal of Animal Science and Biotechnology, 10, 76.
  63. https://doi.org/10.1186/s40104-019-0373-2
  64. Wu, G. (2010). Functional amino acids in growth, reproduction, and health. Advances in Nutrition, 1(1), 31–37.
  65. https://doi.org/10.3945/an.110.1008
  66. Wu, G., Bazer, F. W., Dai, Z., Li, D., Wang, J., & Wu, Z. (2014). Amino acid nutrition in animals: protein synthesis and beyond. Annual Review of Animal Biosciences, 2, 387–417.
  67. https://doi.org/10.1146/annurev-animal-022513-114113
  68. Xia, M., Peng, J., Cui, C., Gu, Q., Zhou, L., Wang, C., Sun, H., Peng, J., & Wei, H. (2021). Effect of gestation dietary methionine-to-lysine ratio on methionine metabolism and antioxidant ability of high-prolific sows. Animal Nutrition, 7(3), 849-858.
  69. https://doi.org/10.1016/j.aninu.2021.02.006
  70. Younis, F. E., & Abd-Elazem, R. A. (2019). Effect of supplementation of rumen-protected amino acids to barki sheep on some blood parameters. International Journal of Environment, Agriculture and Biotechnology (IJEAB), 4(5), 2456-1878.
  71. https://doi.org/10.22161/ijeab.45.40
  72. Zhai, W., Peeples, E. D., Wang, X., Gerard, P. D., Olanrewaju, H. A., & Mercler, Y. (2016). Effect of dietary lysine and methionine supplementation on Ross 708 male broilers from 21 to 42 day of age (III): serum metabolites, hormones and their relationship with growth performance. Journal of Applied Poultry Research, 25(2), 223-231.
  73. https://doi.org/10.3382/japr/pfw004
  74. Zhang, W., Cui, H., Ding K., Zhou, K., Li, Y., Hoque, S.A.M, Min, L., & Zhu, Z. (2023). Carboxylated ε-poly-l-lysine improves post-thaw quality, mitochondrial functions and antioxidant defense of goat cryopreserved sperm. Biology, 12, 231.
  75. https://doi.org/10.3390/biology12020231
  76. Zoca, S. M., Northrop-Albrecht, E. J., Walker, J. A., Cushman, R. A., & Perry, G. A. (2022). Proteomic analyses identify differences between bovine epididymal and ejaculated spermatozoa that contribute to longevity. Theriogenology, 184, 51-60.
  77. https://doi.org/10.1016/j.theriogenology.2022.02.021