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This study aimed to investigate the inhibitory leverage of the aqueous and alcoholic extracts from Asparagus (Asparagus officinalis L.) root as in inhibiting inhibit the growth of the pathogenic bacterium, and fungus in vitro study. Concentrations of 50, 100, and 150  were used as aqueous extract  and alcohol extract  of Asparagus root  against selected organisms, which tested using a drilling method, adding a 0.2 ml aqueous extract and alcohol extract  of Asparagus root in every per well. Also 0.2 ml of distilled water and methanol alcoholic add as control and tetracycline as an antibiotic. GC/MS test results showed that the Asparagus root extract (ARE) contains various major phytochemical compounds such as flavonoids, phenols, alkaloids, glycosides, steroid, resin, saponins, and tannins. Also, it was observed that the alcoholic ARE had a higher inhibitory effect than aqueous ARE of Escherichia coli and Staphylococcus aureus. The highest diameter of inhibition at alcoholic extract concentrations of 50, 100 and 150 ( were 28, 32, 35, and 26, 31, 35 mm respectively for aqueous ARE, compared to other concentrations. Whereas the effect of the aqueous extract was higher for the Pseudomonas 17, 25 and 34 mm compared to other concentrations, except for the antibiotic, which obtained the highest value (41mm). Aqueous extract concentrations 50, 100 and 150 showed higher efficiency than alcohol extract, antibiotic and control; when used against the fungus Aspergillus niger, and pathogenic yeast Candida albicans, the retarding diameters were 14, 17 and 23, and 11, 13 and 15 mm, respectively.


Antimicrobial activity Asparagus officinalis Bioactive components Quantitative analysis

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How to Cite
Abbas, R. J. ., & Al-Subaihawi, R. A. . (2022). The Effect of Aqueous and Alcoholic Extract of Asparagus (Asparagus officinalis L.) Roots on the inhibition of some Bacteria and Fungi . Basrah Journal of Agricultural Sciences, 35(2), 119–131.


  1. Aljazy, N. A., Al-Mossawi, A. E.-B. H., & Al-Rikabi, A. K. (2019). Study of antibacterial activity of some date seed extracts. Basrah Journal of Agricultural Sciences, 32, 247-257.
  3. Al-Aloosi, O. W. J. (2014). The role of Jasmonic acid and salinity stress in enhancing some modifications in the secondary metabolism level in callus cultures of Vinca rosea L. M. Sc. Thesis, College of Science, University of Tikrit, 195pp. (In Arabic)
  4. Al-Janae’e, A. M. S., Ali, A. H., & Al-Edany, T. Y. (2017). Efficacy of some aromatic plant extracts on treating the eggs of the common carp (Cyprinus carpio L.) against fungal infection in comparison with traditional fungicide malachite green. Basrah Journal of Agricultural Sciences, 30(2), 59-71.
  6. Al-Snafi, A. E. (2017). Pharmacology and therapeutic potential of Euphorbia hirta (Syn: Euphorbia pilulifera) A-review. Journal of Pharmacy, 7(3), 7-20. https:// dio:10.9790/3013-0703010720.
  7. Churanya, O., Nitra, N., Watoo, P., Waraporn, P., Neti, W., & Kornkanok, I. (2017). Quantification of Saponins in Asparagus racemosus by HPLC-Q-TOF-MS/MS. Natural Product Communications, 12(1), 7-10.
  8. https:// dio:10.1177/1934578X1701200103
  9. Chouhan, A. D., & Pragati, S. P. (2014). Studies on antibacterial potential of Asparagus racemosus extract against bacteria causing UTI. International Journal of Pharmaceutical Research, 6, 102-107.
  11. Drost, D. (2018). A single application of phosphorus at planting improves long-term Asparagus root growth and yield. International Journal of Vegetables Sciences, 24(2), 146-159.
  13. Gupta, P. K., Mital, B. K., & Grag, S. K. (1996). Characterization of lactobacillus acidophilus strains for use as dietary adjunct. International Journal of Food Microbiology, 29, 105-109.
  14. Harbone, G. B. (1984). Phytochemical methods. A guide to modern techniquces of plants analysis. 2nd. Edition. Chapman and Hall .London, Newyork, 287pp.
  15. Iqbal, M. Bibi, Y., Raja, N. I., Ejaz, M., Hussain, M., Yasmeen, F., Saira, H., & Imran, M. (2017). Review on therapeutic and pharmaceutically important medicinal plant Asparagus officinalis L. Journal of Plant Biochemistry & Physiology, 5(1), 180.
  17. Ismaeli, A. K., Taha, R. M., Mohajer, S., & Banisalam, B. (2016). In vitro regeneration and comparison of phenolic content, antioxidant and antityrosinase activity of in vivo and in vitro grown Asparagus officinalis. Sains Malaysiana Journal, 45(3), 373-376.
  19. Kobus-Cisowska, J., Górnaś, P., Szymanowska D., Szczepaniak, O. M., Gramza-Michałowska, A., Dominik, D., Kulczyński, B., Szulc, P., & Górna, P. (2019). Composition of polyphenols of Asparagus spears (Asparagus officinalis) and their antioxidant potential. Ciência Rural, 49(4), 1-8.
  21. Mbiantcha, M., Kamanyi, A., Teponno, R. B., Tapondjou, L. A., Watcho, P., & Nguelefack, T. B. (2011). Analgesic and anti-inflammatory properties of extracts from the Bulbils of Dioscorea bulbifera L. var sativa (Dioscoreaceae) in mice and rats. Evidence-Based Complementary and Alternative Medicine, 2011, Article ID 912935, 9pp.
  23. Minh, N. P., Them, L. T., Dang, N. C., Ho, T. P., Hieu, N. T., & Luan, L. Q. (2019). Investigation of Asparagus officinalis shoot size and drying affecting to herbal tea production. Journal of Pharmaceutical Sciences and Research, 11(2), 267-272.
  24. Pravala, E. N., & Venkafesham, V. (2017). Evaluation of Asparagus officinalis for anti- diabetic activity in rats. International Journal of Innovative Pharmaceutical Science and Research, 5(7), 1-9.
  26. Reid, R., & Smith, F. A. (2000). The limits of sodiumcalcium interaction in plant growth. Australian Journal Plant Physiology, 27, 709-715.
  28. Seema, S., Rajendra, J., & Daharwal, S. J. (2019). An updated review on herbal drug in wound healing. Research Journal of Pharmacy and Technology, 12(6): 3089-3097.
  29. Shah, M. A., Abdullah, S. M., Khan, M. A., Nasar, G. & Saba, I. (2014). Antibacterial activity of chemical constituents isolated from Asparagus racemosus. Bangladesh Journal of Pharmacology, 9(1), 3pp.
  31. Shaha, P., & Anurag, B. (2017). Pharmacological profile of Asparagus racemosus: A review. International Journal of Current Microbiology and Applied, Sciences, 6(11), 1215-1223.
  33. Shariff, Z. U. (2001). Modern Herbal Therapy for Common Ailments. Nature Pharmacy Series (Volume 1). Spectrum Books Limited, Ibadan, 91pp.
  34. Singh, N., Gupta, S., & Rathore, V. (2017). Comparative antimicrobial study of ethanolic extract of leaf and rhizome of Curcuma longa Linn. Pharmacognosy Journal, 9(2), 208-212.
  36. SPSS (2015). SPSS users guide statistics version 23.
  37. Tinrat, S., & Sila-Asna, M. (2017). Phytochemical screening and influence of extraction solvents on antioxidant and antimicrobial activities of Asparagus racemosus Willd. Root. International Journal of Phytomedicine, 9(2), 343-351.
  38. https://doi:10.5138/09750185.2085
  39. Zhang, H., Birch, J., Pei, J., Ahmed, I. A. M., Yang, H., Dias, G., El-Aty, A. M. A., & Bekhit, A. E. (2019). Identification of six phytochemical compounds from Asparagus officinalis L. root cultivars from New Zealand and China using UAE-SPE-UPLC-MS/MS: Effects of extracts on H2O2-induced oxidative stress. Nutrients, 11(1), 107.