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Abstract
This study evaluated three male date palm varieties, namely, Red-Ganami, Red-Ganami + Khakri Al-Adi and Red-Ganami + Al-Canary, in three age of spathes (early, late and middle) in a private orchard at Al-Seebah district, Basrah province. The plants were subjected to two pollination dates immediately after opening of female flowers and three days after opening of female flowers. The contents of auxin-like substances, gibberellin and abscisic acid in the flowers were determined. The second pollination date resulted in the highest contents of auxins, gibberellins and abscisic acid, with values of 24.305, 138.660 and 12.355 µg.gm-1, respectively. The mixture of Red-Ganami + canary cultivars had the highest concentrations of auxin 24.512 µg.gm -1 and gibberellins 136.842 µg.gm-1, whereas Red-Ganami had the highest concentration of abscisic acid 3.554 μg.gm-1. Early age of spathes showed significant differences in the concentrations of auxin and abscisic acid, with average values of 26.015 and 14.031 µg.gm-1, respectively. Late postmenopausal age recorded the highest concentration of gibberellins at 136.842 μg.gm-1. For the two- and three-way interactions, a statistically significant superiority was observed in most of the study coefficients. In conclusion, the contents of auxins, gibberellins and abscisic acid significantly differed with the pollination date and the pollin cultivar combinations showed the highest concentrations of these substances. The different ages of male puberty also affected pollination and fertilization
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References
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References
Abd, A. M., Altemimy, I. H. H., & Altemimy, H. M. A. (2020). Evaluation of the effect of nano-fertilization and disper osmotic in treating the salinity of irrigation water on the chemical and mineral properties of date palm (Phoenix dactylifera L.). Basrah Journal of Agricultural Sciences, 33(1), 68–88.
https://doi.org/10.37077/25200860.2020.33.1.06
Alaida, M. F., & Aldhebiani, A. Y. (2022). Comparative study of the morphological characteristics of Phoenix dactylifera L. cultivars in Al-Madinah Al-Munawarah-Saudi Arabia. BMC Plant Biology, 22(1).
https://doi.org/10.1186/s12870-022-03841-0
Ali-Dinar, H., Mohammed, M., & Munir, M. (2021). Effects of pollination interventions, plant age and source on hormonal patterns and fruit set of date palm (Phoenix dactylifera L.). Horticulturae, 7(11), 427.
https://doi.org/10.3390/horticulturae7110427
Altemimy, H. M. A., Altemimy, I. H. H., & Abed, A. M. (2019). Evaluation the efficacy of nano-fertilization and Disper osmotic in treating salinity of irrigation water in quality and productivity properties of date palm Phoenix dactylifera L. IOP Conference Series. Earth and Environmental Science, 388(1), 012072.
https://doi.org/10.1088/1755-1315/388/1/012072
Angelovici, R., Galili, G., Fernie, A. R., & Fait, A. (2010). Seed desiccation: a bridge between maturation and germination. Trends in Plant Science, 15(4), 211–218.
https://doi.org/10.1016/j.tplants.2010.01.003
Berríos, J., Illanes, A., & Aroca, G. (2004). Spectrophotometric method for determining gibberellic acid in fermentation broths. Biotechnology Letters, 26(1), 67–70.
https://doi.org/10.1023/b:bile.0000009463.98203.8b
Cheruth, A. J., Kurup, S. S., & Subramaniam, S. (2015). Variations in hormones and antioxidant status in relation to flowering in early, mid, and late varieties of date palm (Phoenix dactylifera) of United Arab Emirates. The Scientific World Journal, 2015, 1–8.
https://doi.org/10.1155/2015/846104
Crozier, A., Loferski, K., Zaerr, J. B., & Morris, R. O. (1980). Analysis of picogram quantities of indole-3-acetic acid by high performance liquid chromatography-fluorescence procedures. Planta, 150(5), 366–370.
https://doi.org/10.1007/bf00390171
El-Kosary, S., Hmmam, I., Gadalla, E. G., & Qenawy, Y. G. (2023). Morphological, physicochemical, and molecular evaluation of twenty-three date palm males growing in Aswan governorate. Basrah Journal of Agricultural Sciences, 36(1), 90–106.
https://doi.org/10.37077/25200860.2023.36.1.08
Farooq, M. A., Zhang, X., Zafar, M. M., Ma, W., & Zhao, J. (2021). Roles of reactive oxygen species and mitochondria in seed germination. Frontiers in PlantScience, 12.
https://doi.org/10.3389/fpls.2021.781734
Fattahi, R., Mohammadzedeh, M., & Khadivi-Khub, A. (2014). Influence of different pollen sources on nut and kernel characteristics of hazelnut. Scientia Horticulturae, 173, 15–19.
https://doi.org/10.1016/j.scienta.2014.04.031
Fukuda, H. (2004). Signals that control plant vascular cell differentiation. Nature Reviews Molecular Cell Biology, 5(5), 379–391.
https://doi.org/10.1038/nrm1364
Ghnimi, S., Umer, S., Karim, A., & Kamal-Eldin, A. (2017). Date fruit (Phoenix dactylifera L.): An underutilized food seeking industrial valorization. NFS Journal, 6, 1–10.
https://doi.org/10.1016/j.nfs.2016.12.001
Gómez-Cadenas, A., Pozo, O. J., García-Augustín, P., & Sancho, J. V. (2002). Direct analysis of abscisic acid in crude plant extracts by liquid chromatography-electrospray/tandem mass spectrometry: Determination of abscisic acid in plant extracts. Phytochemical Analysis: PCA, 13(4), 228–234.
https://doi.org/10.1002/pca.640
Hamzah, H. A., & Aubied, I. A. (2019). Effect of pollen grains and growth regulator NAA on some fruit characterization of date palm Phoenix dactylifera L cultivar. Sultani. Al-Qadisiyah Journal For Agriculture Sciences, 9(1), 36–42.
https://doi.org/10.33794/qjas.2019.162663
Karim, K., Karim, A., Hamza, H., Manar, A., Karmous, C., & Bettaieb, T. (2022). Effect of pollination technique on agronomic and physicochemical characteristics of date palm fruits (Phoenix dactylifera L.) cv. deglet Nour. Korean Journal of Horticultural Science & Technology, 40(2), 121–133.
https://doi.org/10.7235/HORT.20220012
Pandolfini, T. (2009). Seedless fruit production by hormonal regulation of fruit set. Nutrients, 1(2), 168–177.
https://doi.org/10.3390/nu1020168
Panoli, A., Martin, M. V., Alandete-Saez, M., Simon, M., Neff, C., Swarup, R., Bellido, A., Yuan, L., Pagnussat, G. C., & Sundaresan, V. (2015). Auxin import and local auxin biosynthesis are required for mitotic divisions, cell expansion and cell specification during female gametophyte development in Arabidopsis thaliana. PloS One, 10(5), e0126164.
https://doi.org/10.1371/journal.pone.0126164
Pieck, M., Yuan, Y., Godfrey, J., Fisher, C., Zolj, S., Vaughan, D., Thomas, N., Wu, C., Ramos, J., Lee, N., Normanly, J., & Celenza, J. L. (2015). Auxin and tryptophan homeostasis are facilitated by the ISS1/VAS1 aromatic aminotransferase in Arabidopsis. Genetics, 201(1), 185–199.
https://doi.org/10.1534/genetics.115.180356
Qadir, A., Shakeel, F., Ali, A., & Faiyazuddin, M. (2020). Phytotherapeutic potential and pharmaceutical impact of Phoenix dactylifera (date palm): Current research and future prospects. Journal of Food Science and Technology, 57(4), 1191–1204.
https://doi.org/10.1007/s13197-019-04096-8
Salomón-Torres, R., Sol-Uribe, J. A., Valdez-Salas, B., García-González, C., Krueger, R., Hernández-Balbuena, D., Norzagaray-Plasencia, S., García-Vázquez, J. P., & Ortiz-Uribe, N. (2020). Effect of four pollinating sources on nutritional properties of Medjool date (Phoenix dactylifera L.) seeds. Agriculture, 10(2), 45.
https://doi.org/10.3390/agriculture10020045
Santner, A., Calderon-Villalobos, L. I. A., & Estelle, M. (2009). Plant hormones are versatile chemical regulators of plant growth. Nature Chemical Biology, 5(5), 301–307.
https://doi.org/10.1038/nchembio.165
Shahsavar, A. R., & Shahhosseini, A. (2022). The metaxenia effects of different pollen grains on secondary metabolites enzymes and sugars of ‘Piarom’ date palm fruit. Scientific Reports, 12(1), 1–14.
https://doi.org/10.1038/s41598-022-14373-w
Suhim, A. A., Awad, K. M., Jaffer, O. N., & Abass, M. H. (2023). The impact of salicylic and jasmonic acid in mitigating salinity stress on date palm Phoenix dactylifera L. barhi CV. Basrah Journal of Agricultural Sciences, 36(1), 120–130.