Article Sidebar

Download
PDF
Statistic
Read Counter : 392 Download : 201

Downloads

Download data is not yet available.

Main Article Content

Abstract

This study was carried out to assess the genetic variation between two local Iraqi (white and grey) ducks by RAPD-PCR technique using five random primers. The total  number of bands  that shown by the primers (APO-08, APOF-O9, APO-10, APO-16, APO-18) was 63, 53, 79, 81, 68 for local white  ducks, while it was 62, 61, 66, 69, 58 for the local gray ducks respectively. Additionally, all primers showed 59 common bands. OPA-08 primer showed the highest number of common   bands (22) while APF-09 primer  resulted the lowest number of common bands (4 bands). The overall number of specific bands in local white ducks were higher than those of local grey ducks (285 and 257 bands, respectively). The average of similarity ratio between local ducks was (35.76) while individually, APO-08 primer showed the highest similarity ratio (70.4%) comparing with the lowest similarity percentage (14.04%) that represented by OPF-09 primer.

Keywords

Genetic variation Polymorphism RAPD Markers Ducks

Article Details

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

How to Cite
Al-Kurdi, M. A., Al-Shaheen, S. A., & Al-Asadi, M. H. (2019). Use of RAPD Markers Technique to Evaluate Genetic Variation in Two Types of Local Ducks. Basrah Journal of Agricultural Sciences, 32(2), 1–6. https://doi.org/10.37077/25200860.2019.176
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX

References

  1. Alyehhodi, R.R..; Kumar, S.; Panda, B.K.; Singh, P. S.; Choudhary & Jaiswal, G. (2010). Molecular genetic characterization of Moti native duck using RAPD markers. J. Appl. Anim. Res., 37(1): 19-23.
  2. Basha, H.A., Abd el Naby, W.S.H & Heikal, H.S. (2016). Genetic diversity and phylogenetic relationship among three duck breeds and geese using RAPD
  3. markers. Adv. Anim. Vet. Sci., 4(9): 462-467.?
  4. Dunnington, E.A., Plotsky., Y.H., Aberfeld, A., Kirls, T., Goldberg, A., Lavi, V., Cohanes, A., Siegel, P.B. & Hillel, J. (1990). DNA Fingerprinting chickens selected for high and low body weight for 31 generation. J. Anim. Genet.., 21: 247-257.
  5. El-Araby, I.E. & Saleh, A.A. (2016). Assessment of phylogenetic relationship and genetic variability among some duck breeds Using RAPD-PCR as Molecular Markers. Alexandria J. Vet. Sci., 51(1): 174-179.?
  6. El-Gendy, E.A.; Helal, M.A.; Goher, N.H & Mostageer, A. (2005). Molecular characterization of genetic biodiversity in ducks, using RAPD-PCR analysis. Arab J. Biotech., 8(2): 253-264.
  7. FAO (2014). FAO statistical yearbook: Africa Food and Agriculture, Accra: 168pp.
  8. Gilbert, D.A.; Lehman, N.D.; Brien, S.J. & Wayne, R.K. (1990). Genetic Fingerprinting reflects population difference in the California channel island fox. Nature., 344: 764-766.
  9. Kulikova, I.V.; Chelomina, G.N. & Zhuravlev, Y.N. (2003). Low genetic differentiation of and close evolutionary relationships between Anas platyrhynchos and Anas poecilorhyncha: RAPD–PCR Evidence. Russ. J. Genet., 39(10): 1143-1151.?
  10. Kumar, N.S. & Gurusubramanian, G. (2011). Random amplified polymorphic DNA (RAPD) markers and its applications. Sci. Vis., 11(3): 116-124. ?
  11. Li, H.; Yang, N.; Chen, K.; Chen, G., Tang, Q.; Tu, Y.; & Ma. Y. (2006). Study on molecular genetic diversity of native duck breeds in China. World's Poult. Sci. J., 62(4): 603-611.?
  12. Lynch, M. (1990). The similarity index and DNA fingerprinting. Mol. Bio.Evol.,7: 478-484.
  13. MIFFAF (Ministry for Food, Agriculture, Forestry). (2013). Primary Statistics of food, Agriculture, Forestry and Fisheries, Sejong, Korea.
  14. Nei, M. (1972). Genetic distance between populations. Am. Nat., 106(949): 283-292.?
  15. Nelson K. & Soule, M. (1987). Genetical conservation of exploited fishes. Pp: 345-368 In. Ryman N. & F Utter, F. (Eds.). Population genetics and fishery management. Univ. Washington Press. 418pp.
  16. Ogah, D.M & Momoh, O.M. (2014). Analysis of genetic diversity of Nigeria indigenous Muscovy duck ecotypes using RAPD Marker. PAT., 10(2): 225-232.
  17. Sankhyan, V. (2007). Molecular genetic characterization of Indian runner native duck. M. Sc. Thesis. Indian Vet. Res. Inst. Izantngar. (cited from Basha et al., 2016)
  18. Sharma R..; Kishore, A.; Mukesh M.; Ahlawat S.; Maitra A.; Pandey A.K. & Tantia, M.S. (2015). Genetic diversity and relationship of Indian cattle inferred from microsatellite and mitochondrial DNA markers. BMC Genetics., 16: 1-12.